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Plant Sciences

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Louisiana State University

LSU Doctoral Dissertations

Theses/Dissertations

2016

Marker-assisted selection

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Identification Of Genes Associated With Resistance To Brown Rust In Sugarcane And Prevalence Of One Major Gene, Mavir Carolina Avellaneda Barbosa Jan 2016

Identification Of Genes Associated With Resistance To Brown Rust In Sugarcane And Prevalence Of One Major Gene, Mavir Carolina Avellaneda Barbosa

LSU Doctoral Dissertations

Development of resistant cultivars is the main control measure against sugarcane brown rust caused by Puccinia melanocephala. Durability is uncertain, since the pathogen possesses adaptive ability to overcome host plant resistance. A differential gene expression study utilizing suppressive subtraction hybridization was conducted to improve understanding of brown rust resistance mechanisms in sugarcane. The expression patterns of 11 unigenes representing biosynthetic pathways, defense-related genes, and signaling genes were analyzed in L 99-233, a cultivar exhibiting quantitative resistance, L 01-299, a resistant cultivar with the major resistance gene Bru1, and two susceptible cultivars, Ho 95-988 and L 09-125, at 24 h, 48 …