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Probing The Porphyromonas Gingivalis Nitrosative Stress Response Network, Alexandra Galina

Theses and Dissertations

Objective: To persist in the oral cavity, the perio-pathogen Porphyromonas gingivalis must have an efficient nitrosative stress response mechanism. To better understand how P. gingivalis responds to nitrite (NO₂^-) we must determine what genes are involved in the nitrosative stress response network.

Methods: Through genomic screening, a list of genes was generated that were hypothesized to be part of the nitrosative stress response network and believed to be involved in nitrate/nitrite metabolism or redox state of the cells. Protein coding regions were replaced with the ermF sequence by allelic exchange mutagenesis. 15 mutants were created …

Applications Of Urban Land Evaluation And Site Assessment (Ulesa) In Chesterfield County, Virginia, Emily Hastings

Theses and Dissertations

Urban and suburban agriculture is increasing in popularity across the United States. However, many of these farms are short-lived due to a combination of human factors (e.g., managerial, economic, cultural) and environmental or spatial factors (e.g., zoning, soil quality, distance to markets). Geospatial analysis, in the form of suitability modeling, could aid in locating suitable sites for these activities. Land Evaluation and Site Assessment (LESA) is a suitability modeling technique that generates a score for agricultural land use in traditionally rural spaces. This research explores adapting this method to encompass the complexities of placing diverse, in-ground agriculture in urban and …

The Hepatic Immunological Pattern Shaped By Dominant-Subdominant Cellular Interactions Creates A Collective Function Beyond The Function Of Each Cellular Constituent To Orchestrate Progression Of Hepatocellular Carcinoma, Nicholas J. Koelsch

Theses and Dissertations

Abundance of data on the role of inflammatory immune responses in the progression or inhibition of hepatocellular carcinoma (HCC) has failed to offer a curative immunotherapy for HCC. This is largely because of taking reductionist approaches and missing the collective function of the hepatic immune system by focusing on specific immune cell types. To this end, we propose that focusing on the dominant-subdominant patterns of the immune cells would allow understanding of the mechanism by which a collective immune function emerges. To identify the collective immune function through a systems immunology perspective, we performed high-throughput analysis of snRNAseq data collected …

Treponema Denticola Synthesizes C-Di-Amp And Encodes The Cdaa-Type Diadenylate Cyclase Cdaa, Claire R. O'Brien

Theses and Dissertations

Periodontitis is a form of oral disease characterized by dysbiosis of the oral microbiome, leading to inflammation, bone resorption, and in severe cases, entire tooth loss, affecting 42% of adults in the US. One of the bacteria most associated with periodontal disease progression is Treponema denticola (Td), an oral spirochete which inhabits the mouth in small quantities during health but which can dominate the biofilms that form during periodontal disease. The ability of Td to survive in a disease environment and contribute to the progression of disease requires the use of robust signaling networks. Analysis of Td cultures …

Epithelial Membrane Protein 3 (Emp3b) Is A Novel Regulator Of Sympathetic Nervous System Development In Zebrafish., Jessica M. White

Theses and Dissertations

Notable progress has been made in understanding the development of the neural crest (NC). Neural crest cells (NCCs) can be divided into 4-5 subpopulations. A major lineage among trunk NC derivatives are sympathoadrenal (SA) cells, which give rise to the sympathetic nervous system (SNS). Trunk NCCs follow a ventromedial migration pathway where signals derived from the dorsal aorta instruct migrating NCCs to a SA cell fate. Defects in NC development can result in syndromes known as neurocristopathies. Neuroblastoma is one of the most common forms of pediatric cancer affecting nearly 800 infants each year in the United States (Takita et …

Characterizing The Effects Of Antiandrogens And Senolytics To Enhance The Therapeutic Response To Castration-Resistant Prostate Cancer, Justin M. Silverman

Theses and Dissertations

Prostate cancer is the most frequently diagnosed cancer in males and the second most common cause of cancer deaths. Androgen deprivation therapy, whether through surgical or chemical castration, is the mainstay for treatment of advanced prostate cancer; however, despite an initial response, most patients eventually develop a progressive PSA rise, and castration- sensitive prostate cancer gives rise to castration-resistant prostate cancer. The standard of care therapy includes the antiandrogens such as enzalutamide and abiraterone acetate as well as the microtubule poison, docetaxel, and various immunotherapies; however, while prostate cancer research is progressing, there continues to be a compelling need for …

Association Of Interpersonal Trauma And Polygenic Risk Scores With Depressive Symptoms In College Students, Rowan K. O'Hara

Theses and Dissertations

Major depression is considered a complex trait influenced by both polygenic risk factors and environmental exposures, such as childhood trauma. This study applied statistical genetic methods to calculate aggregate genetic risk for major depression to predict depressive symptoms scores in a college student sample. Data were from the Spit for Science (S4S) study in which college students from a large urban university self-reported interpersonal trauma (IPT) exposure prior to college and depressive symptoms from the past month (N = 7502; ancestry group: 20% African [AFR], 12% Admixed Americas [AMR], 10% East Asian [EAS], 49% European [EUR], 8% South Asian [SAS]). …

Investigating Salinity Intrusion Effects On Sapelo Island, Georgia Using Benthic Macroinvertebrate Bioindicators, Raymond Paul Kidder Ii

Electronic Theses and Dissertations

Coastal wetlands are threatened by the compounding effects of sea level rise and historical land modification. Historically, much of Sapelo Island, Georgia was deforested and drained using a series of ditches and canals to create arable land fit for agriculture and to provide drainage to the inhabited parts of the island. Today, this network of artificial channels is believed to be facilitating the movement of saltwater from the nearby estuary into freshwater areas on the interior of the island. This study aimed to better understand the frequency and magnitude of salinity intrusion events along with their resulting impacts to the …

Novel Strategies In Head And Neck Tumor Therapy; Contribution Of Senescence In Therapy Response, And Resistance Development After Cisplatin Treatment, Fereshteh Ahmadinejad

Theses and Dissertations

Therapeutic outcomes achieved in head and neck squamous cell carcinoma (HNSCC) patients by concurrent cisplatin-based chemoradiotherapy initially reflect both tumor regression and tumor stasis. However, local, and distant metastasis and disease relapse as well as cisplatin-induced resistance are common in HNSCC patients. In the current work, we demonstrate that cisplatin treatment induces senescence in both p53 wild- type HN30 and p53 mutant HN12 head and neck cancer models. We also show that tumor cells can escape from senescence both in vitro and in vivo. We further established a cisplatin-resistant cell line from HN30 parental cell line that underwent brief senescence …

A Standardized Pipeline For Isolation And Assembly Of Genomes From Symbiotic Bacteria In Whole Louse Genomic Sequence Data., Mohammad Mikail I. Bala

Theses and Dissertations

Many insects are known to harbour intracellular and heritable bacteria (endosymbionts), which provide their hosts with adaptive traits. Whole insect gDNA shotgun sequencing projects often sequence the genome of endosymbiont, in addition to the insect’s genome. There are approximately 600 whole genome shotgun libraries from insects available on the public repository (NCBI), which can be mined to obtain endosymbiont genomes. The assembly and annotation of endosymbiont genomes can contribute towards the exploration of their role as obligate symbiotic partners. However, de novo assembly of an endosymbiont genome, continues to be challenging, when the host and/or enteric bacterial gDNA is present …

Evaluating How Acidic Solutions Affect The Microhardness Of Bioceramic Sealers, Colton Fischer

Theses and Dissertations

Introduction: With growing use of bioceramic sealers (BCS) in root canal therapy, there have been challenges when retreating BCS cases. Weak acids may be effective in removing BCS and their efficacy as a solvent can be measured by their influence on the microhardness of BCS. Microhardness can be used as an indirect measurement of material hardness. The purpose of this study was to investigate the microhardness of BCS when placed in contact with various acidic solutions to assess their potential use as a solvent for retreatment procedures.

Methods: EndoSequence BC Sealer (Brasseler USA) was injected into four 10 × 3mm …

Dune Building Dynamics Impact Cross-Island Connectivity And Barrier Island Characteristics, Alexander Sabo

Theses and Dissertations

Located at the land-sea interface, barrier islands are important protective features that reduce wave energy and erosion from mainland areas. Further, barrier islands provide habitat for many different organisms and serve as popular areas for recreation and tourism. As sandy landforms, barrier islands are easily disturbed, but often recover after disturbance as a result of island plant communities, specifically dune building grasses. Disturbance on barrier islands is driven by storms, tides, and overwash events. After disturbance has occurred, dune grasses colonize the dunes allowing for dune building and habitat restoration. On the Virginia barrier islands, there are three dominant dune …

Growing The Future Of Agriculture: An Examination Of Knowledge-Sharing And Community Building In The Fresh Produce And Floral Council, Oscar Guzman

Theses and Dissertations

This study examines knowledge-sharing practices within a community of practice (CoP) through a case study of the Fresh Produce and Floral Council (FPFC). The study sought to investigate how the FPFC promoted agricultural knowledge and expertise, impacted its members' social relationships and knowledge-sharing practices, communicated clear roles and expectations, and provided valuable and accessible resources. A mixed-methods approach was employed to investigate the research questions, which involved conducting a survey and utilizing descriptive statistics, frequencies, and Spearman correlations to analyze both the Likert scale and open-ended responses. The findings suggested that the FPFC has a knowledge-sharing culture promoting active participation …

The Effect Of Weekly Training Load On Match Result In American Professional Soccer, Cameron M. Horsfall

Electronic Theses and Dissertations

BACKGROUND: During a professional soccer season, training load (TL) will fluctuate due to the need to prepare for the next competition. Based on the technical staff’s daily and weekly evaluation, TL will be adjusted as they prepare the team and maximize individual performance and achieve a favorable result. Therefore, the programming of each match week (MW) TL may promote a favorable result. PURPOSE: The purpose of this study was to examine variation in daily GPS-derived TL variables based on match result using the Match Day Minus (MD-) system. This study provides descriptive TL data yet to be reported in …

Effects Of Occupational Tasks And Personal Protective Equipment On Static And Functional Balance Among Career Firefighters, Petra Kis

Electronic Theses and Dissertations

BACKGROUND: Musculoskeletal injuries are commonly incurred on the fireground among firefighters. Most injuries are attributable to overexertion, fall, jump, slip, and trip mechanisms. These injuries may be caused by many factors, including the independent and collective effects of wearing personal protective equipment (PPE) and the fatigue induced by performing occupational tasks. Therefore, the purpose of this study was to investigate the effect of PPE and fire suppression tasks on static and functional balance among firefighters. METHODS: 32 healthy male career structural firefighters (Age: 31.8±9.5 yr) volunteered for this study. Static and Functional Balance Tests (FBT) were performed in …

The Relationship Between Chronotypes And Cardiovascular Disease Risk Factors Among Law Enforcement Officers, Alyssa N. Fail

Electronic Theses and Dissertations

Much of the data associated with cardiovascular disease risk factors and circadian rhythms are done on shift workers; due to their line of work typically causing circadian disruption and sleep loss (Loef et al., 2019). Previous research incorporating chronotype and its influence on shift work has been explored; however, there is limited research focusing on law enforcement officers. Additionally, no known research has investigated the effect of chronotype on CVD risk factors among law enforcement officers. The aim of this study was to investigate the cardiovascular disease risk factors in law enforcement officers and their relationship with chronotypes. This cross-sectional …

Evaluating Blood Pressure And Hypertension Awareness In Municipal Workers In The Southeast Us, Kayden R. Mcalvin

Electronic Theses and Dissertations

The primary purpose of this study was to describe municipal workers’ blood pressure (BP) status and awareness; the secondary purpose was to examine the association between BP misconception and department. Municipal employees in a southeastern rural community (n=75) were asked to participate in a 15-minute survey, including the BASIS Hypertension Awareness survey, and blood pressure measurements to determine hypertension risk. Results show a significant difference in diastolic blood pressure (DBP) between police with a higher DBP of 87.64 ± 10.9 mm Hg and fire departments 78.98 ± 11.1 mm Hg (p=0.005), a significant difference in DBP between city hall/public works …

Hepatic Transcriptomic Assessment Of Sprague Dawley Rats In Response To Dietary Perfluorobutane Sulfonate (Pfbs) Ingestion, Isaac Appiah

Electronic Theses and Dissertations

Perfluorobutane sulfonate (PFBS) is a short-chain PFAS that is considered to be a less toxic replacement for the rather more toxic long-chain perfluorooctane sulfonate (PFOS). Its numerous industrial applications and widespread presence in the environment have raised environmental and health concerns because of growing evidence associating adverse health effects and certain liver diseases to PFBS exposure. The study goal was to investigate whether dietary ingestion of PFBS would induce liver injuries, damage, inflammations, and oxidative stress. To achieve these goals, Sprague-Dawley rats were assigned into three PFBS dietary treatment groups (0, 50, and 100 PPM) for 11 weeks. After this …

Comparative Analytics On Chilli Plant Disease Using Machine Learning Techniques, Sai Abhishta Roy Seelam

Electronic Theses and Dissertations

This thesis concerns the detection of diseases in chilli plants using machine learning techniques. Three algorithms, viz., Convolutional Neural Network (CNN), Recurrent Neural Network (RNN), and Multi-Layer Perceptron (MLP), and their variants have been employed. Chilli-producing countries, India, Mexico, China, Indonesia, Spain, the United States, and Turkey. India has the world’s largest chilli production of about 49% (according to 2020). Andhra Pradesh (Guntur) is the largest market in India, where their varieties are more popular for pungency and color. This study classifies five kinds of diseases that affect the chilli, namely, leaf spot, whitefly, yellowish, healthy, and leaf curl. A …

Endogenous Antioxidant Response And Lipid Peroxidation Levels In Muscle Tissue Of The Cownose Ray (Rhinoptera Bonasus), Bailey Berry

Electronic Theses and Dissertations

Oxidative stress is the result of an imbalance between reactive oxygen species (ROS) and antioxidant response within cells. ROS generation is a natural consequence of ETC activity within the mitochondria and is associated with aerobic metabolism. Oxidative stress data on elasmobranch muscle is limited, with previous studies primarily focusing on mammalian and teleost muscle. Elasmobranch skeletal muscle is generally composed of large proportions of white muscle fibers (anaerobic), low proportions of red muscle fibers (aerobic), and low proportions of pink muscle fibers (anaerobic). The cownose ray, a benthopelagic batoid species, presents an interesting model of study because it contains relatively …

The Effect Of Genetic Relatedness On Mate Selection And Spatial Distribution In The American Alligator, Alligator Mississippiensis, Mark Hoog

Electronic Theses and Dissertations

To date, most genetic studies on the American alligator (Alligator mississippiensis) have focused on population genetics and multiple paternity. There have not been any studies looking at how relatedness affects alligator behavior and movement. This study focused on three main questions: 1) what is the genetic diversity among alligators in this location? 2) can parentage be accurately defined among parents and offspring? and 3) how does individual relatedness affect their spatial distribution? We examined the relatedness of 174 unique individuals from the Okefenokee Swamp Park, in South Georgia, USA. In addition, we placed Telonics^® satellite tags on …

The Effect Of Tempo On Prone Hamstring Exercise - An Exploration Of Unilateral Bias, Derick A. Anglin

Electronic Theses and Dissertations

Hamstring injuries are common muscle-skeletal injuries with a high recurrence rate. While unmodifiable risk factors (e.g., previous injury) vary, recent evidence has questioned the utility of eccentric exercise in preventing hamstring strains. Furthermore, whether eccentric exercise can transfer to sprinting’s kinetic and kinematic requirements is of question. Therefore, this investigation examines exercise tempo (fast vs. slow eccentric phase), movement strategy (bilateral vs. unilateral), and external load characteristics (coupled vs. independent) bias concentric (CON) and eccentric (ECC) biceps femoris (BF) mean electromyography (mEMG). METHODS: Twenty college-aged students aged 18 – 33 (M = 23.4, SD = 3.12 years) with …

Use Of Hematological Markers To Assess Physiological Condition And Health Status In Free-Ranging Sand Tiger Sharks (Carcharius Taurus), Chestina N. Craig

Electronic Theses and Dissertations

The contents of blood can provide information about the physiological condition and health of vertebrates. This study seeks to better understand the stress physiology and blood bacteria presence of the sand tiger shark (Carcharius taurus), as sharks are known to have unique physiology and immune systems. In this study the blood metabolites glucose, lactate, and ketones (3-hydroxybuteric acid and acetoacetate), were used to understand how biotic and abiotic factors affect the acute stress response to capture and handling. Metabolite concentrations from blood plasma were analyzed using colorimetric assays. Glucose and ketones showed no significant responses to capture and …

Dna Methylation And The Response To Infection In Introduced House Sparrows, Melanie Gibson

Electronic Theses and Dissertations

Epigenetics is the study of molecular modification of a genome without changing its base pairs. The most studied type of epigenetic mechanism is DNA methylation, which is capable of turning a gene “on” or “off.” Epigenetic potential is the capacity to which an individual can have methylation on its genome. The more CpGs available, the greater the epigenetic potential. In invasive species, genetic variation has been observed to be paradoxical: not much of it exists on a genomic level, but epigenetically, phenotypic variation can occur. The focus on shift in gene expression in this study is on Toll-Like Receptor 4 …

Effect Of Menstrual Cycle Phase On The Rewarding Properties Of Alcohol In Women, Annie K. Griffith

Theses and Dissertations--Psychology

Compared with men, women are disproportionately affected by alcohol, including greater risks of behavioral impairment and relapse. One likely mechanism underlying this disparity is fluctuations of ovarian hormones, particularly estradiol (E2), across phases of the menstrual cycle. Pre-clinical and clinical studies have shown that higher E2 levels correlate with heavier patterns of alcohol consumption, suggesting E2 may modulate drinking. This relationship is thought to be mediated by the rewarding properties of alcohol. The present thesis tested the hypothesis that elevated E2 during the late follicular phase would increase the rewarding properties of alcohol. Fifty young-adult women participated in a within-subjects …

The Effects Of Extended Fructose Access On Relative Value And Demand For Fructose, Saccharin, And Ventral Tegmental Stimulation, Megan Halloran

Theses and Dissertations--Psychology

Globally, food addiction (FA) is a growing area of research and is largely attributed to the availability of foods that are both energy dense and high in fats and sugars. Further, it has been suggested, that sugar and fat, when consumed frequently, have properties similar to drugs of abuse. While the validity of FA is questioned, researchers have drawn parallels between substance use disorder (SUD) and FA. For example, sugar binge models emphasize craving, withdrawal and binging as primary components of FA, which are also hallmarks of SUD. Additionally, both natural rewards, like sugars, and drug rewards act on the …

Community Supported Agriculture Values: A Comparison Across Groups, Thomas B. Pierce

Theses and Dissertations--Agricultural Economics

Community Supported Agriculture (CSA) programs offer consumers the chance to share the risk with farming operations while gaining access to fresh, local foods. While research on CSA shareholder values such as share affordability or the local impact of participation has been conducted in the past decade and beyond, less attention has been paid to direct value-mapping of the shareholders themselves. This research seeks to determine consumer values around the CSA purchasing decision using the Best-Worst Scaling Approach. Based on a focus group discussion with CSA shareholders in a university wellness voucher program, we used affinity diagramming to develop a list …

The Effects Of Cucurbitacin B And Silmitasertib On Metastasis And Itga6 Using The Zebrafish Tumor-Xenograft, Alexandra Griffis

Electronic Theses and Dissertations

Ninety percent of cancer deaths are resultant from the metastasis of cancer cells. When cancer cells translocate through blood vessels or the lymphatic system, they may form tumors outside of their primary site. The processes of metastasis can begin quickly; after onset, metastasis is unforgiving, as it does not participate with the body’s physiological systems in an orderly way. In the past, our lab produced results indicating that a cell adhesion molecule, Integrin Alpha-6, may contribute to cancer cells' ability to metastasize. Integrins mediate interactions between the cell and the Extracellular Matrix (ECM), regulating cell attachment and cell migration. With …

Remote Sensing Of Georgia Tidal Marsh Habitats Using Aerial Photography And Planetscope Satellite Imagery, Harrison M. Currin

Electronic Theses and Dissertations

Globally, tidal marshes cover about 90,800 km. Within the state of Georgia tidal marshes are primarily located behind the barrier islands and total 1,619 km². The combination of high salinity environments and daily inundation, and being dependent on river output, make these dynamic systems. Tidal marshes provide numerous ecosystem services such as carbon and nitrogen sequestration, flood control, coastal protection, and numerous biogeochemical processes. Due to their unique position, tidal marshes are under threat from sea level rise, drought, coastal development, and large-scale disturbance events. Tidal freshwater marshes are especially susceptible to these threats due to their geographic …

Middle Savannah River: An A/R/Tographic Ecopedagogical Ethnography Experimenting With Rhizomatic Perspectives, Lisa Augustine-Chizmar

Electronic Theses and Dissertations

This research is an experiment in perspective. Using the four commonplaces (Schwab, 1978), I practiced letting the Savannah River teach me what there is to know about the water, the land, the people, and the other entities that depend on ki through artistic, ethnographic, and ecopedagogical lenses. The ethnographic findings describe the social actors that depend on ki and give a voice to the River. The a/r/tographic findings display the River on a canvas map through two hundred years using paint, clay, photography, video, abstract acrylics, and fabric. Together, these methods contribute to a unique ecopedagogical journey. This word cloud …