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The Design And Testing Of Fluorescence-Based Sensors For Phosphate And Phosphonate, Sara Hassanzadehroknabadi

Electronic Theses and Dissertations

Inorganic phosphate (Pi) is an essential compound for all living things, as it serves as a component of nucleic acids and ATP, which are required for plant growth and proliferation. However, when excess Pi enters the aquatic environment, it has severe detrimental effects through the induction of harmful algal blooms (HAB), which negatively impact aquatic life, the quality of drinking water as well as recreational activities. It is therefore critical to have fast, sensitive, and inexpensive sensors for Pi that quickly and quantitively measure its concentration in a field setting.

Phosphonates or organophosphorus compounds are phosphoric acids possessing alkyl or …

Structure And Mechanics Of Biomembrane Mimics In The Presence Of Vitamin E, Mitchell Dipasquale

Electronic Theses and Dissertations

Over the course of this dissertation, the fundamental behaviours and interactions between various analogs of vitamin E and biologically-relevant membranes will be explored. These analogs include the biologically active form, α-tocopherol; the most naturally abundant form γ-tocopherol; an oxidized product, α-tocopheryl quinone; and the synthetic stabile form, vitamin E acetate.

Beginning in Chapter 1, a foundation in membrane biophysics is established from a historical progression of the field. The importance of biomembranes is argued and a deep connection is established between structure and function. Key structural features of lipid assemblies, and role of lipid composition is defined, and with the …

Mother Knows Best: Mechanistic Aspects Of Foraging Preference And Differential Arrestment Of Gravid Females: A Case Study On Blow Flies (Diptera: Calliphoridae), Vanessa Elizabeth Owen

Electronic Theses and Dissertations

Behavioural plasticity is a concept that describes a change in an organism’s behaviour due to their exposure to stimuli that differ from their usual environment. For instance, females of many insect species alter their behavioural responses when exposed to differing substances that they utilize for feeding, oviposition, and/or mating. Behaviours such as the frequency of walking, tasting, grooming exhibited by flies can be associated with the detection and acceptance of a suitable medium. For this thesis, the arrestment and behavioural response of gravid adult females of three species of blow flies (Diptera: Calliphoridae), Lucilia sericata Meigen, Phormia regina Meigen, and …

The Bouba/Kiki Effect On Gendered Objects In The Arabic Language, Ghadir Nassereddine

Electronic Theses and Dissertations

People are more likely to associate the nonword bouba with a round shape, and kiki with a sharp shape, an effect known as the Bouba/Kiki effect (BK) (Ramachandran & Hubbard, 2001). Relatedly, female names are associated with a round shape whereas male names are associated with a sharp shape (Sidhu & Pexman, 2015) and this effect extends to grammatically gendered nonwords in French (Sidhu, Pexman, & Saint-Aubin, 2019). These associations have not been examined in Arabic, or with real words with a grammatical gender. The present study addresses this gap by examining the influences of letter sound …

Mortality In Interspecific Hybrids Of Nasonia Vitripennis And Nasonia Giraulti, Bonnie Cobb

Electronic Theses and Dissertations

Nasonia is a parasitoid wasp genus that serves as an emerging model for studying speciation due to an incompatibility between nuclear and mitochondrial genomes between sister taxa. Short generation times, easy rearing in a lab setting, producing large amounts of progeny, and whole genome sequencing make Nasonia is an excellent candidate for studying incomplete reproductive isolation. Nasonia have five chromosomes and exhibit haplo-diploid sex determination in which fertilized eggs develop into diploid females and unfertilized eggs develop into haploid males. Recessive phenotypes are hidden in diploid females but are laid bare in haploid males as there is no interaction between …

Amblyomma Quadricavum (Schulze, 1941) (Acari:Ixodidae): First Description Of The Nymphal Stage And Phylogenetic Assessment Of Its Taxonomic Status, Ashleigh V. Riggs

Electronic Theses and Dissertations

The hard tick (Acari: Ixodidae) Amblyomma quadricavum (Schulze, 1941) is a parasite of snakes and is endemic to the Caribbean region. Morphological characters of this species, namely its rudimentary eye structures, have led to debate about its taxonomic status. The species was originally assigned to the genus Aponomma. However, with revisions of the genus Aponomma, and the recent creation of new genera created for some basal former-Aponomma, it has been suggested that perhaps Amblyomma quadricavum might be a close relative of Robertsicus elaphensis, the Trans-Pecos rat snake tick of the southwest United States and New Mexico, another eyeless former-Aponomma species …

Applying Conservation Genomic Techniques To Guide Management Of The Reticulated Flatwoods Salamander (Ambystoma Bishopi), Miranda Gaupp

Electronic Theses and Dissertations

The Reticulated flatwoods salamander (Ambystoma bishopi) is a federally endangered amphibian endemic to the longleaf-pine ecosystem of the southeastern U.S. This study used analyses of single-nucleotide polymorphism (SNP) data, collected from 2,255 unique individuals across 5 breeding seasons, spread across the known extant range of A. bishopi, to characterize the genetic diversity and demographics of populations, genetic relationships among populations, and patterns and spatial extents of gene flow, and to evaluate potential effects of management on A. bishopi’s resiliency. Population structure was strongly hierarchical, with individual breeding ponds (n = 38) acting as semi-connected subpopulations …

Epigenetic Buffering In Introduced House Sparrows, M. Ellesse Lauer

Electronic Theses and Dissertations

Epigenetic buffering, as an environmentally induced increase in variance of epigenetic states that increases phenotypic variation to buffer populations against decreased fitness, may be a factor that resolves the genetic paradox of introduced species. DNA methylation is a molecular mechanism that could facilitate epigenetic buffering by changing in response to environmental stress. Therefore, epigenetic buffering can be detected through increased variance in DNA methylation in novel or heterogeneous environments. Introduced house sparrows (Passer domesticus) have well-documented phenotypic changes with low genetic diversity, high epigenetic diversity, and high variance in DNA methylation that provide a characteristic signature of epigenetic buffering. …

Development Of Novel Pyridazine Derivatives And Drug Delivery Systems Against Dengue, Janae A. Culmer

Electronic Theses and Dissertations

The lack of approved vaccines, medications and treatment regimens has significantly contributed to the rapid spread of mosquito-borne viruses such as Dengue and Zika virus. The complex immunopathology of these viruses presents limitations for the development and implementation of a definitive, safe and effective approach to combat infections.Previous research has demonstrated that vector control strategies such as the elimination of larval habitats, larviciding with insecticides, the use of biological agents and the application of adulticides have been unsuccessful in the reduction of viral transmission leading to the need for the continued development of antivirals. This research proposes an approach for …

Behavior And Chemical Signals As Markers Of Colony Identification In Argentine Ants (Linepithema Humile), Stephanie A. Rohrbach

Electronic Theses and Dissertations

Argentine ants, Linepithema humile, are a highly successful invasive species around the globe and are especially prominent in states such as California and the southeastern United States. L.humile have a unique form of unicoloniality, called “supercolonies”. L. humile can detect colonymates through scent markers in their outer cuticle. With these chemical markers, ants will exhibit high aggression if they smell different from one another. In our study, we performed aggression assays among ten different nest sites and analyzed their CHCs through gas chromatography mass spectrometry, or GC-MS, analysis. For our behavior results, while within-nest interactions displayed low aggression as …

Hemocyte-Mediated Immune Response To A Bacterial Infection In The Cat Flea (Ctenocephalides Felis), Melanie Muñoz

Electronic Theses and Dissertations

Despite their continued medical and veterinary importance, the vectorial capacity of fleas (Order Siphonaptera) is often underestimated. Fleas are best described as ectoparasites of a variety of mammalian and aviary hosts. In humans, fleas are infamous for the transmission of several detrimental agents, including those that cause plague, murine typhus, and cat-scratch disease. Although their significance in flea immune responses remains unknown, phagocytic immune cells, known as hemocytes, are important players in the cell mediated immune response of insects. Among invertebrates, hemocytes provide defense against foreign microbes via phagocytosis, cellular encapsulation, and the production of humoral immune factors. As mentioned …