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Assessment Of Three Grape Varieties' Resistance To Five Viruses And Assembly Of A Novel Grapevine Vein Clearing Virus Genome, Michael Ryan Kovens
Assessment Of Three Grape Varieties' Resistance To Five Viruses And Assembly Of A Novel Grapevine Vein Clearing Virus Genome, Michael Ryan Kovens
MSU Graduate Theses
Over 65 viruses are known to infect grapevines, more than any other agricultural crop. Growth, yield, and quality of virus-infected vines suffer, reducing the profitability to grape growers. Therefore, it is critical to identify grape cultivars resistant to these viruses which can be utilized to protect more susceptible varieties from infection. Wine grape cultivar Norton (Vitis aestivalis) and Chambourcin (V. vinifera hybrid), and rootstock St. George (V. rupestris) have been known to be disease tolerant. In the first study, Norton and Chambourcin were infected with Grapevine fleck virus (GFkV), Grapevine leafroll-associated virus 3 (GLRaV-3), Grapevine virus A (GVA), and Grapevine …
Investigation Of Nocifensive And Cellular Effects Of Dihydroergotamine In A Model Of Chronic Migraine, Jennifer Elise Denson
Investigation Of Nocifensive And Cellular Effects Of Dihydroergotamine In A Model Of Chronic Migraine, Jennifer Elise Denson
MSU Graduate Theses
The goal of my study was to investigate the nocifensive behavioral and cellular effects of dihydroergotamine (DHE) and sumatriptan in a model of sustained trigeminal activation. Trigeminal nerves provide sensory innervation to the head and face and their activation has been implicated in migraine pathology. The most commonly prescribed medications for migraine treatment are the triptan class of drugs. However, triptans are not an effective therapy for all migraine patients and some develop triptan resistance. Ergot-derivatives, including DHE, could be a potential alternative for this subpopulation of patients. To cause activation of the trigeminal system, adult male Sprague Dawley rats …
L-Carnitine And Pyruvate Inclusion In Diluents For Cold-Stored Stallion Spermatozoa, Daniel Scott Potter
L-Carnitine And Pyruvate Inclusion In Diluents For Cold-Stored Stallion Spermatozoa, Daniel Scott Potter
MSU Graduate Theses
Spermatozoa survivability is of utmost importance in delivering equine semen for artificial insemination. Semen extender supplementation may allow for increased survivability. L-carnitine (CARN) has been studied for its role in the mobilization of fatty acids. Two experiments (EX1/EX2) assessed the effects of CARN and pyruvate (P) on spermatozoa survival. Total (TM) and progressive motility (PM), velocity and direction of movement (VAP, VSL, VCL, and elongation) were assessed by CASA, membrane status (SYBR 14/PI stains) and acrosome status (FITC-PSA stain) by flow cytometer. In EX1, 4 ejaculates were collected from 2 stallions. Aliquots were diluted in skim-milk glucose extender (SKMG) or …
Management And Production Practices Of The Missouri Beef Cattle Industry, Jordan Lee Kinder
Management And Production Practices Of The Missouri Beef Cattle Industry, Jordan Lee Kinder
MSU Graduate Theses
A survey of beef cattle producers in Missouri was conducted to evaluate the management and production practices of the beef cattle industry. Production and demographic data from 112 (response rate of 5.9%) were analyzed to identify areas in need of future research and education. The web-based survey was conducted through Survey Monkey© and distributed by the Missouri Cattlemen's Association to a list of their members. Responding producers were located in 65 of the 114 counties in Missouri, with 63.3% located north of Interstate 44 and 36.7% south of Interstate 44. The majority (47.3%) of producers were between ages 31 and …
Effects Of Low Concentrations Of Carbon Nanotubes On Growth And Gas Exchange In Arabidopsis Thaliana, Rajeeva Voleti
Effects Of Low Concentrations Of Carbon Nanotubes On Growth And Gas Exchange In Arabidopsis Thaliana, Rajeeva Voleti
MSU Graduate Theses
The effect of pure single-walled carbon nanotubes (SWCNTs) on plant growth and gas exchange was investigated in Arabidopsis thaliana. To date there has been no research on the effects of SWCNTs on whole plant physiology. A. thaliana seeds were directly grown in growth medium containing SWCNTs concentrations of 24.93micrograms/ml and 53.55 micrograms/ml. control plants were grown in media containing distilled water. I determined growth by measuring dry mass of plants. I determined gas exchange by measuring photosynthetic rates, stomatal conductance, transpiration rates, and water use efficiency. I also examined the following physiological mechanisms that would limit plant growth: ATP and …
The Effects Of Phosphorus And Nitrogen Fertilization On Tall Fescue Nutrient Content, Yield And Baleage Quality, Cheyenne Adele Arthur
The Effects Of Phosphorus And Nitrogen Fertilization On Tall Fescue Nutrient Content, Yield And Baleage Quality, Cheyenne Adele Arthur
MSU Graduate Theses
Tall fescue (Festuca arundinacea Schreb.) is a cool season perennial grass commonly used in year-round forage production systems. Producers often wrap large round bales of tall fescue in plastic. The fermented product of this practice, termed baleage, allows the forage to be stored for later feeding. During periods of rapid growth in the spring and the fall, the nutrient content of tall fescue can be imbalanced and cause nutritional disorders in grazing animals. To improve the nutrition of tall fescue, it is known that fertilization of phosphorus (P) can help improve leaf nutrient content of P, magnesium (Mg), and calcium …
Functional Connection Between Yeast Dynamin And Retromer At The Endosome, Christopher Robert Trousdale
Functional Connection Between Yeast Dynamin And Retromer At The Endosome, Christopher Robert Trousdale
MSU Graduate Theses
Intracellular trafficking from the late endosome to Golgi in cells is termed retrograde transport, essential for recycling of important macromolecules including cell membrane receptors. Retrograde transport is regulated by a family of proteins known as the "Retromer” composed of 5 VPS proteins (Vps5, Vps17, Vps26, Vps29, and Vps35). Retromer acts as the coat proteins for vesicles emerging from late endosomes. Loss of Retromer function has been previously implicated in both Parkinson's and Alzheimer's disease. Vps1, a yeast dynamin-like protein, plays a role in intracellular trafficking. Vps1 has been shown to localize at the endocytic sites to promote pinching off of …
Dynamin Association With Clathrin And Its Physiological Roles At The Golgi And Targeting Mechanism To The Golgi, Shiva Kumar Goud Gadila
Dynamin Association With Clathrin And Its Physiological Roles At The Golgi And Targeting Mechanism To The Golgi, Shiva Kumar Goud Gadila
MSU Graduate Theses
Vps1 (Vacuole protein sorting 1), a dynamin-like protein in yeast, is implicated in diverse membrane trafficking pathways and localized to many organelles, including the Golgi. It has been proposed that Vps1 is functionally linked to clathrin heavy chain 1 (Chc1), but the question of how, where, and when they function together remains unknown. Lines of evidence suggest that Chc1 and Vps1 are located at the late Golgi. Therefore, I hypothesized that Vps1 binds to Chc1 and functions together at the Golgi for efficient Golgi-to-endosome membrane trafficking. Using the yeast-two hybrid assay, I present evidence that Vps1 binds to the C-terminal …
Reintroduction Biology Of Head-Started Ornate Box Turtles, Eric R. Sievers
Reintroduction Biology Of Head-Started Ornate Box Turtles, Eric R. Sievers
MSU Graduate Theses
The ornate box turtle (Terrapene ornata) is a prairie-dwelling species that has experienced population declines, especially near the northern edge of its range. In order to provide supporting research for a reintroduction program at the Upper Mississippi River National Wildlife and Fish Refuge in northwestern Illinois, I compared the relative success of different approaches to reintroduction. Specifically, I tested the null hypothesis that reintroduced turtles exhibited equal reintroduction success when, 1) released at a site that is known to support a viable ornate box turtle population, 2) ‘soft-released' in a fenced enclosure at a site where very few ornate box …
Calcitonin Gene-Related Peptide Promotes Peripheral And Central Trigeminal Sensitization, Lauren Elise Cornelison
Calcitonin Gene-Related Peptide Promotes Peripheral And Central Trigeminal Sensitization, Lauren Elise Cornelison
MSU Graduate Theses
Temporomandibular joint disorder is characterized by peripheral and central sensitization of trigeminal nociceptive neurons. Although CGRP is implicated in the development of central sensitization by stimulating glial activation via its receptor, the mechanism by which CGRP promotes and maintains sensitization of trigeminal nociceptive neurons is not well understood. The goal of my study was to investigate the role of calcitonin gene-related peptide (CGRP) on the initiation and maintenance of a nocifensive withdrawal response to mechanical stimulation following activation of primary trigeminal sensory neurons. For my studies, I used adult male Sprague Dawley rats that were injected with CGRP alone or …
Maximum Entropy Modeling Of Indiana Bat (Myotis Sodalis) Maternity Roost Habitat, Joseph Robert Lemen
Maximum Entropy Modeling Of Indiana Bat (Myotis Sodalis) Maternity Roost Habitat, Joseph Robert Lemen
MSU Graduate Theses
Since 1967, the Indiana bat (Myotis sodalis) has been on the U.S. Endangered Species list due to disruption of hibernating bats in caves, summer habitat degradation, and more recently, the onset of White-nose Syndrome. The purpose of this study is to evaluate landscape variables associated with Indiana bat maternity roost trees in an attempt to better understand what factors play a role in their distribution in north central Missouri. I tracked reproductive female Indiana bats to 20 different primary and secondary roost trees; these are roosts that had multiple bats visit them on multiple occasions. GPS location data for these …
Nesting Success And Parental Behavior Of The Prothonotary Warbler (Protonotaria Citrea) In Southwestern Missouri, Kathryn Marie Siverly
Nesting Success And Parental Behavior Of The Prothonotary Warbler (Protonotaria Citrea) In Southwestern Missouri, Kathryn Marie Siverly
MSU Graduate Theses
The breeding biology and singing rates of Prothonotary Warblers (Protonotaria citrea) were studied in southwestern Missouri in 2014 and 2015, using wooden nesting boxes. Data were collected on clutch and brood sizes, the numbers of young fledged, parental care and male singing rates. Five pairs were studied over the course of the two summers. Of 28 eggs laid, 14 young fledged. In 2015, significant flooding resulted in the loss of all young and eggs for that year. First clutches were larger than second clutches. Observations suggest females may contribute more to the care of offspring, in terms of foraging and …
Effect Of Ingestion By Alligator Snapping Turtles (Macrochelys Temminckii) On Seeds Of Riparian Vegetation, Jean Pierre Elbers
Effect Of Ingestion By Alligator Snapping Turtles (Macrochelys Temminckii) On Seeds Of Riparian Vegetation, Jean Pierre Elbers
MSU Graduate Theses
The alligator snapping turtle (Macrochelys temminckii) is a large freshwater turtle that inhabits many lotic water bodies in the Southeastern United States. The species consumes primarily fish but also consumes large amounts of vegetation including seeds of common persimmon (Diospyros virginiana), tupelos (Nyssa sp.), willow oak (Quercus phellos), and pecan (Carya illinoensis). Captive specimens of M. temminckii were fed samples of the above-mentioned seeds to assess how the species affects ingested seeds in order to evaluate the potential role this species may play as a seed disperser. The proportion of seeds defecated intact varied with species (57−99 %), was lowest …
Geology And Wine In Missouri: Spatial Analysis Of Terroir Using A Geographic Information System And Remote Sensing, Kathryn Nora Barnard
Geology And Wine In Missouri: Spatial Analysis Of Terroir Using A Geographic Information System And Remote Sensing, Kathryn Nora Barnard
MSU Graduate Theses
The concept of terroir, based on the French word meaning 'sense of place,' suggests that flavor and quality of wine is associated with certain physical characteristics of the earth as well as viticulture and viniculture practices. The physical characteristics of terroir include soil, geology, topography, and climate. Designated American Viticultural Areas (AVAs) are discrete appellations in the United States that have well-established and historic viticultural practices, but the relationship between terroir and AVA appellations is complex. Wineries are a growing industry in the United States and the use of a GIS and remote sensing has proved useful in many other …
Growth And Population Assessment Of Spotted Bass (Micropterus Punctulatus) In Beaver, Table Rock, And Bull Shoals Lakes, Northern Arkansas-Southern Missouri, Jeremy Troy Risley
Growth And Population Assessment Of Spotted Bass (Micropterus Punctulatus) In Beaver, Table Rock, And Bull Shoals Lakes, Northern Arkansas-Southern Missouri, Jeremy Troy Risley
MSU Graduate Theses
Little research has been conducted on spotted bass, Micropterus punctulatus, even though it is a popular recreational fish. Most previous research was on young-of-the-year fish and interactions among other species in reservoirs, with little research on adult spotted base in the Ozarks region or other regions of the United States. The objective of this study was to compare the growth and population characteristics of spotted bass within and among Beaver, Bull Shoals and Table Rock Lacks. Growth, abundance, and conditions within and among reservoirs were similar. However, spotted bass in Beaver Lake did not reach as great lengths as those …
Habitat Use Of Urban Bats In Springfield, Missouri, Sarah Jane Robertson
Habitat Use Of Urban Bats In Springfield, Missouri, Sarah Jane Robertson
MSU Graduate Theses
During the summers of 1998-2000, the Anabat II bat detector system was placed in a mosaic of habitats within Springfield, Missouri and surrounding rural areas in southern Missouri to compare the activity indices of nine bat species between urban and rural environments. A total of 1,876 echolocation call sequences was recorded, identified to species and analyzed; 1,066 were from 42 Springfield sites, and 810 were from 22 rural locations. For all species combined, urban bat activity tended to be higher than rural activity, but those differences were only significant at the reading taken 3 hours after sunset. Eptesicus fuscus activity …