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Perkinsus Marinus Extracellular Protease Modulates Survival Of Vibrio Vulnificus In Eastern Oyster (Crassostrea Virginica) Hemocytes, Bd Tall, Jf La Peyre, Et Al, M Faisal
Perkinsus Marinus Extracellular Protease Modulates Survival Of Vibrio Vulnificus In Eastern Oyster (Crassostrea Virginica) Hemocytes, Bd Tall, Jf La Peyre, Et Al, M Faisal
VIMS Articles
The in vitro effects of the Perkinsus marinus serine protease on the intracellular survival of Vibrio vulnificus in oyster hemocytes were examined by using a time-course gentamicin internalization assay. Results showed that protease-treated hemocytes were initially slower to internalize V. vulnificus than untreated hemocytes. After 1 h, the elimination of V. vulnificus by treated hemocytes was significantly suppressed compared with hemocytes infected with invasive and noninvasive controls. Our data suggest that the serine protease produced by P. marinas suppresses the vibriocidal activity of oyster hemocytes to effectively eliminate V. vulnificus, potentially leading to conditions favoring higher numbers of vibrios in …
Estimation Of Bacterial Respiration And Growth Efficiency In The Ross Sea, Antarctica, C. A. Carlson, N. R. Bates, H. W. Ducklow, D. A. Hansell
Estimation Of Bacterial Respiration And Growth Efficiency In The Ross Sea, Antarctica, C. A. Carlson, N. R. Bates, H. W. Ducklow, D. A. Hansell
VIMS Articles
Seawater cultures were conducted in large volume (36 l) gas impermeable tri-laminate bags for the purpose of empirically deriving bacterial growth efficiency (BGE) and carbon conversion factors (CCF) in the south central Ross Sea. This experimental design allowed for concomitant measurements of metabolic reactants (loss of total and dissolved organic carbon [TOC and DOG]) and products (gain of total carbon dioxide [TCO2] and bacterial biomass) to be made from a single incubation vessel. Some previous studies have relied on proxy measurements (e.g. O-2, H-3-thymidine incorporation and cell abundance) to determine BGE and CCF rather than direct carbon measurements. Our experimental …
Bacterial Growth In Experimental Plankton Assemblages And Seawater Cultures From The Phaeocystis Antarctica Bloom In The Ross Sea, Antarctica, H. W. Ducklow, C. Carlson, Walker O. Smith Jr.
Bacterial Growth In Experimental Plankton Assemblages And Seawater Cultures From The Phaeocystis Antarctica Bloom In The Ross Sea, Antarctica, H. W. Ducklow, C. Carlson, Walker O. Smith Jr.
VIMS Articles
A series of seawater culture experiments was carried out during the Phaeocystis antarctica bloom in the Ross Sea polynya (76.5 degrees S, 180 degrees W; November to December 1994 and December 1995 to January 1996) to examine bacterioplankton growth and derive empirical factors for estimating bacterial production rates. Bacterial growth was exponential over 3 to 10 d in all experiments, at rates of ca 0.1 to 0.7 d(-1), even in persistently cold waters (-2 to + 1 degrees C). Growth rates were lower in the early part of the bloom (early to mid-November) and highest during the period of peak …
The Bacterial Component Of The Oceanic Euphotic Zone, H. W. Ducklow
The Bacterial Component Of The Oceanic Euphotic Zone, H. W. Ducklow
VIMS Articles
Bacteria in the open sea remote from land are sustained strictly on local sources of organic production which should make understanding their nutrition and growth regulation easier than in nearshore systems, estuaries and lakes. Until now, a paucity of data from geographically isolated oceanic sites prevented ready :interpretation. In the past decade investigation of bacterial properties in oceanic systems has increased rapidly, stimulated in part by large oceanographic programs like the Joint Global Ocean Flux Study. Here I review comprehensive investigations of bacterial biomass and production dynamics in the subarctic north Atlantic and north Pacific, oligotrophic gyres in both oceans, …
Bacterioplankton Growth Responses To Temperature And Chlorophyll Variations In Estuaries Measured By Thymidine:Leucine Incorporation Ratio, Fk Shiah, Hw Ducklow
Bacterioplankton Growth Responses To Temperature And Chlorophyll Variations In Estuaries Measured By Thymidine:Leucine Incorporation Ratio, Fk Shiah, Hw Ducklow
VIMS Articles
To identify the biochemical response of heterotrophic bacterioplankton to changing environmental conditions, seasonal and diel cycles of bacterial protein and DNA synthesis rates were estimated in temperate estuarine habitats from H-3-leucine (Leu) and H-3-thymidine (TdR) incorporation rates. Several short-term temperature manipulation experiments (5 to 35 degrees C) and 2 mesocosm experiments were performed to examine the effects of temperature and substrate supply on the ratio of Leu:TdR, respectively. The molar ratio of Leu to TdR varied about 5-fold (5.6 to 29.5) in the field and the values of the ratio were lower and more constant during high temperature (>25 …
Enumeration Of Enterococcus Sp. Using A Modified Me Method, Mw Rhodes, H Kator
Enumeration Of Enterococcus Sp. Using A Modified Me Method, Mw Rhodes, H Kator
VIMS Articles
A modified mE medium (mEI) containing the chromogenic substrate indoxyl-beta-D-glucoside to detect beta-D-glucosidase activity was evaluated with respect to specificity and recovery of enterococci from environmental eaters. Extending incubation from 24 to 48 h improved enterococci recovery but 77% of the colonies classified as non-target were confirmed as enterococci. Randomly chosen enterococcal isolates from sewage, exposed in microcosms containing 0.22 mu m membrane filtered fresh or estuarine water, exhibited differences in persistence as a function of exposure treatment. Decreasing the concentration of or eliminating indoxyl-beta-D-glucoside from mE did not significantly affect recovery of purified isolates.
Effects Of Sunlight On Bacteriophage Viability And Structure, Ke Wommack, Rt Hill, Ta Muller, Rr Colwell
Effects Of Sunlight On Bacteriophage Viability And Structure, Ke Wommack, Rt Hill, Ta Muller, Rr Colwell
VIMS Articles
Current estimates of viral abundance in natural waters rely on direct counts of virus-like particles (VLPs), using either transmission or epifluorescence microscopy. Direct counts of VLPs, while useful in studies of viral ecology, do not indicate whether the observed VLPs are capable of infection and/or replication. Rapid decay in bacteriophage viability under environmental conditions has been observed. However, it has not been firmly established whether there is a corresponding degradation of the virus particles, To address this question, viable and direct counts were carried out employing two Chesapeake Bay bacteriophages in experimental microcosms incubated for 56 h at two depths …
Growth Of Bacterioplankton And Consumption Of Dissolved Organic Carbon In The Sargasso Sea, C. A. Carlson, H. W. Ducklow
Growth Of Bacterioplankton And Consumption Of Dissolved Organic Carbon In The Sargasso Sea, C. A. Carlson, H. W. Ducklow
VIMS Articles
Lability of the bulk dissolved organic carbon (DOG) pool and the amount available to bacterioplankton on short time scales (hours to days) were examined in oligotrophic Sargasso Sea water (near Bermuda). We examined bacterial growth and DOC utilization using seawater culture methodology in combination with measurements of bacterial abundance, cell volume, and DOC. Bulk DOC concentrations were determined by high temperature combustion (HTC) analysis, which proved to be a sensitive method for detecting small changes in natural concentration of DOG. Measurable bacterial growth and DOC utilization only occurred in unamended cultures when initial DDC concentrations were greater than observed in …
Aerobic And Anaerobic Degradation And Mineralization Of C-14 Chitin By Water Column And Sediment Inocula Of The York-River-Estuary, Virginia, Jn Boyer
VIMS Articles
Potential rates of chitin degradation (C(d)) and mineralization (C(m)) by estuarine water and sediment bacteria were measured as a function of inoculum source, temperature, and oxygen condition. In the water column inoculum, 88 to 93% of the particulate chitin was mineralized to CO2 with no apparent lag between degradation and mineralization. No measurable dissolved pool of radiolabel was found in the water column. For the sediment inocula, 70 to 90% of the chitin was degraded while only 55 to 65% was mineralized to CO2. C-14 label recoveries in the dissolved pool were 19 to 21% for sand, 17 to 24% …
Measurement Of Marine Picoplankton Cell-Size By Using A Cooled, Charge-Coupled Device Camera With Image-Analyzed Fluorescence Microscopy, Cl Viles, Me Sieracki
Measurement Of Marine Picoplankton Cell-Size By Using A Cooled, Charge-Coupled Device Camera With Image-Analyzed Fluorescence Microscopy, Cl Viles, Me Sieracki
VIMS Articles
Accurate measurement of the biomass and size distribution of picoplankton cells (0.2 to 2.0-mu-m) is paramount in characterizing their contribution to the oceanic food web and global biogeochemical cycling. Image-analyzed fluorescence microscopy, usually based on video camera technology, allows detailed measurements of individual cells to be taken. The application of an imaging system employing a cooled, slow-scan charge-coupled device (CCD) camera to automated counting and sizing of individual picoplankton cells from natural marine samples is described. A slow-scan CCD-based camera was compared to a video camera and was superior for detecting and sizing very small, dim particles such as fluorochrome-stained …
Evaluation Of Automated Threshold Selection Methods For Accurately Sizing Microscopic Fluorescent Cells By Image-Analysis, Me Sieracki, Se Reichenbach, Kl Webb
Evaluation Of Automated Threshold Selection Methods For Accurately Sizing Microscopic Fluorescent Cells By Image-Analysis, Me Sieracki, Se Reichenbach, Kl Webb
VIMS Articles
The accurate measurement of bacterial and protistan cell biomass is necessary for understanding their population and trophic dynamics in nature. Direct measurement of fluorescently stained cells is often the method of choice. The tedium of making such measurements visually on the large numbers of cells required has prompted the use of automatic image analysis for this purpose. Accurate measurements by image analysis require an accurate, reliable method of segmenting the image, that is, distinguishing the brightly fluorescing cells from a dark background. This is commonly done by visually choosing a threshold intensity value which most closely coincides with the outline …
Survival Of Escherichia-Coli And Salmonella Spp In Estuarine Environments, Mw Rhodes, Hi Kator
Survival Of Escherichia-Coli And Salmonella Spp In Estuarine Environments, Mw Rhodes, Hi Kator
VIMS Articles
Survival of Escherichia coli and Salmonella spp. in estuarine waters was compared over a variety of seasonal temperatures during in situ exposure in diffusion chambers. Sublethal stress was measured by both selective-versus-resuscitative enumeration procedures and an electrochemical detection method. E. coli and Salmonella spp. test suspensions, prepared to minimize sublethal injury, were exposed in a shallow tidal creek and at a site 7.1 km further downriver. Bacterial die-off and sublethal stress in filtered estuarine water were inversely related to water temperature. Salmonella spp. populations exhibited significantly less die-off and stress than did E. coli at water temperatures of <10°C. Although the most pronounced reductions (ca. 3 log units) in test bacteria occurred during seasonally warm temperatures in the presence of the autochthonous microbiota, 102 to 104 test cells per ml remained after 2 weeks of exposure to temperatures of >15°C. Reductions …
End-Products Of Anaerobic Chitin Degradation By Salt-Marsh Bacteria As Substrates For Dissimilatory Sulfate Reduction And Methanogenesis, Jn Boyer
VIMS Articles
The anaerobic pathway of chitin decomposition by chitinoclastic bacteria was examined with an emphasis on end product coupling to other salt marsh bacteria. Actively growing chitinoclastic bacterial isolates produced primarily acetate, H2, and CO2 in broth culture. No sulfate-reducing or methanogenic isolates grew on chitin as sole carbon source or produced any measurable degradation products. Mixed cultures of chitin degraders with sulfate reducers resulted in positive sulfide production. Mixed cultures of chitin-degrading isolates with methanogens resulted in the production of CH4 with reductions in headspace CO2 and H2. The combination of all three metabolic types resulted in the simultaneous production …
Seasonal-Variation In Survival Of Escherichia-Coli Exposed Insitu In Membrane-Diffusion Chambers Containing Filtered And Nonfiltered Estuarine Water, I. C. Anderson, Mw Rhodes, Hi Kator
Seasonal-Variation In Survival Of Escherichia-Coli Exposed Insitu In Membrane-Diffusion Chambers Containing Filtered And Nonfiltered Estuarine Water, I. C. Anderson, Mw Rhodes, Hi Kator
VIMS Articles
Human fecal Escherichia coli isolates were exposed over a seasonal cycle to estuarine water in diffusion chambers filled with double-filtered (0.45 and 0.2 p.m) and nonfiltered water. Laboratory manipulations of E. coli cultures before estuarine exposure were reduced to minimize sublethal stress, and nonselective or resuscitative enumeration techniques were employed to maximize recovery of stressed cells. E. coli was capable of extended survival during in situ exposure to estuarine water, provided eucaryotes were excluded from diffusion chambers. Survival was directly related to temperature in absence of the eucaryote component of the natural microbiota. Although it was not possible to prevent …
In Situ Development Of Sublethal Stress In Escherichia-Coli - Effects On Enumeration, Mw Rhodes, Iris C. Anderson, H Kator
In Situ Development Of Sublethal Stress In Escherichia-Coli - Effects On Enumeration, Mw Rhodes, Iris C. Anderson, H Kator
VIMS Articles
No abstract provided.
Sublethal Stress In Escherichia-Coli - Function Of Salinity, I. C. Anderson, Mw Rhodes, H Kator
Sublethal Stress In Escherichia-Coli - Function Of Salinity, I. C. Anderson, Mw Rhodes, H Kator
VIMS Articles
Sublethal stress in Escherichia coli was detected in various test media after exposure (in vitro) to seawater of various salinities. Stress was measured with an electrochemical detection technique and a,-galactosidase assay. Test media included EC medium, medium A-1, and tryptic soy broth modified to contain lactose for /?-galactosidase assay experiments. Stress was defined as the difference between a predicted electrochemical response time calculated for unstarved cells from a standard curve and the observed electrochemical response time for cells starved in seawater. The higher the salinity, the greater the stress for all test media examined. Stress was most pronounced in EC …
Physiology And Ecology Of Bacteriophages Of Marine Bacterium Beneckea-Natriegens - Salinity, A Zachary
Physiology And Ecology Of Bacteriophages Of Marine Bacterium Beneckea-Natriegens - Salinity, A Zachary
VIMS Articles
The effects of variation in ionic levels on the stability and replication of two bacteriophages (nt-1 and nt-6) host specific for the marine bacterium Beneckea natriegens were examined. Monovalent cations influenced the adsorption of the nt-1 but not the nt-6 phage; however, one-step growth studies showed that NaCl was required for replication of both phage. The NaCl optimum for nt-1 production was 0.25 M NaCl, the same as the growth optimum for B. natriegens. However, the optimum for nt-6 production was 0.16 M NaCl. These NaCl optima for host and phage are at estuarine rather than oceanic levels. The nt-1 …
Isolation Of Bacteriophages Of The Marine Bacterium Beneckea Natriegens From Coastal Salt Marshes, Arthur Zachary
Isolation Of Bacteriophages Of The Marine Bacterium Beneckea Natriegens From Coastal Salt Marshes, Arthur Zachary
VIMS Articles
Bacteriophages of the marine bacterium Beneckea natriegens were isolated from coastal marshes where they were limited to brackish and marine waters. The phages were widely distributed and morphologically diverse in the marshes.