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Articles 91 - 110 of 110
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Human Microvasculature Fabrication Using Thermal Inkjet Printing Technology, Xiaofeng Cui
Human Microvasculature Fabrication Using Thermal Inkjet Printing Technology, Xiaofeng Cui
All Dissertations
The current tissue engineering paradigm is that successfully engineered thick tissues must include vasculature. As biological approaches alone such as VGEF have fallen short of their promises, one may look for an engineering approach to build microvasculature. With the advent of cell printing, one may be able to build precise human microvasculature with suitable bio-ink. Human Microvascular Endothelial Cells (HMVEC) and fibrin were studied as bio-ink for microvasculature construction. Endothelial cells are the only cells to compose the human capillaries and also the major cells of blood vessel intima layer. Fibrin has been already widely recognized as tissue engineering scaffold …
Characterization Of Vascular Smooth Muscle Cell Mechanical And Frictional Properties Using Atomic Force Microscopy, Jason Hemmer
Characterization Of Vascular Smooth Muscle Cell Mechanical And Frictional Properties Using Atomic Force Microscopy, Jason Hemmer
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A working hypothesis within the Laboratory of Vascular Research is that mechanical loading on vascular smooth muscle cells (VSMCs), especially due to solid contact from endovascular devices, contributes to the development of restenosis. In order to better understand the role of mechanical loading on VSMCs in vascular disease development, it is imperative to understand the mechanical properties of VSMCs themselves. To measure the viscoelastic and frictional properties of living VSMCs in an in vitro setting, an atomic force microscope (AFM) was utilized, thereby allowing for mechanical testing of living cells in a fluid environment. In the first phase of research, …
Ecm Stabilization Strategies For Bioprosthetic Heart Valves For Improved Durability, Devanathan Raghavan
Ecm Stabilization Strategies For Bioprosthetic Heart Valves For Improved Durability, Devanathan Raghavan
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Abstract
Approximately 85,000 heart valve replacement surgeries are performed every year in United States and about 300,000 surgeries worldwide. It is estimated that half of them are mechanical valve replacements and the other half bioprosthetic valve replacements. The use of bioprosthetic heart valves is slowly increasing. Bioprosthetic heart valves are made from porcine aortic valves or bovine pericardium. Commercially these bioprostheses are currently crosslinked using glutaraldehyde (GLUT) to prevent tissue degradation and reduce tissue antigenicity. GLUT crosslinks these bioprostheses by stabilizing the collagen present in the tissue via a Schiff base reaction of the aldehyde with the hydroxylysine / lysine …
Vascular Tissue Engineering: The Creation Of Living, Non-Thrombogenic, Functional Blood Vessels Based On Elastin Scaffolds, Aditee Kurane
Vascular Tissue Engineering: The Creation Of Living, Non-Thrombogenic, Functional Blood Vessels Based On Elastin Scaffolds, Aditee Kurane
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Cardiovascular diseases are the leading cause of death worldwide. Blood vessel replacement is a common treatment for vascular diseases such as atherosclerosis, restenosis and aneurysm, with over 300,000 bypass procedures performed each year. However, vein grafts are limited due to their availability. Although synthetic vascular replacements have been successful for large diameter arteries, they have shown minimal success in arteries with diameters <6mm. This is because most synthetic materials induce thrombus formation which, within a few months of implantation, causes failure of the vascular graft due to occlusion. Tissue engineering is a promising approach to the fabrication of non-thrombogenic vascular grafts, but a reliable and expandable cell source for tissue-engineered vascular graft (TEVG) has not been established.
The work presented here is motivated by the current unavailability of an ideal tissue engineered blood vessel replacement. Our overall goal is to create a living tissue engineered vascular graft that is biodegradable, non-thromobogenic, presents low antigenicity and …
Cues For Cellular Assembly Of Vascular Elastin Networks, Chandrasekhar Kothapalli
Cues For Cellular Assembly Of Vascular Elastin Networks, Chandrasekhar Kothapalli
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Elastin, a structural protein distributed in the extracellular matrix of vascular tissues is critical to the maintenance of vascular mechanics, besides regulation of cell-signaling pathways involved in injury response and morphogenesis. Thus, congenital absence or disease-mediated degradation of vascular elastin and its malformation within native vessels due to innately poor elastin synthesis by adult vascular cells compromise vascular homeostasis. Current elastin regenerative strategies using tissue engineering principles are limited by the progressive destabilization of tropoelastin mRNA expression in adult vascular cells and the unavailability of scaffolds that can provide cellular cues necessary to up-regulate elastin synthesis and regenerate faithful mimics …
Engineered Micro-Environments And Vibrational Culture Systems For Vocal Fold Tissue Engineering, Jaishankar Kutty
Engineered Micro-Environments And Vibrational Culture Systems For Vocal Fold Tissue Engineering, Jaishankar Kutty
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Voice is produced by the conversion of aerodynamic energy from exhalation to acoustical energy for voice production by the vocal folds (membranous connective tissue) located in the larynx. The quality of voice depends on the biomechanical properties of the multi-layered vocal fold tissue which derive from its extracellular matrix (ECM) organization and composition. The wound healing response to vocal fold injuries is characterized by scarring and subsequent dysphonia due to alterations in the biomechanical properties of the tissue.
The work presented here is motivated by the importance of voice in maintaining quality of life and the inability of current treatment …
The Spaceflight Environment And The Skeletal System, Eric Bandstra
The Spaceflight Environment And The Skeletal System, Eric Bandstra
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The spaceflight environment presents many challenges to the human body. Bone loss in astronauts is a well known consequence of reduced loading in the weightless environment of low-earth orbit. Recent studies have also indicated that spaceflight relevant types of radiation (at relatively high doses) have deleterious effects on trabecular bone. Both of these represent potential skeletal challenges during long-duration spaceflight. The objective of these studies is to examine the response of bone to models of the spaceflight environment.
Astronauts will likely absorb doses of protons and heavy ions during lengthy missions outside the Earth's magnetosphere. Following exposure to a range …
Chitosan Derivatives For Tissue Engineering, Yongzhi Qiu
Chitosan Derivatives For Tissue Engineering, Yongzhi Qiu
All Dissertations
Chitosan, a naturally occurring polysaccharide, and its derivatives have been widely explored for biomedical applications due to their biocompatibility and biodegradability. In our studies, we developed a series of chitosan derivatives through chemical modifications. These chitosan derivatives not only possess better processibility in scaffolds fabrication, but also show excellent potentials in tissue engineering applications, including blood vessel and bone tissue engineering.
The excellent antithrombogenic property is crucial for vascular engineering applications, especially in engineering small-diameter blood vessels. In our studies, chitosan was chemically modified by phthalization and the phthalized chitosan exhibited great antithrombogenic property. Through a wet- phase-inversion process, tubular …
Radiation-Induced Osteoporosis: Bone Quantity, Architecture, And Increased Resorption Following Exposure To Ionizing Radiation, Jeffrey Willey
Radiation-Induced Osteoporosis: Bone Quantity, Architecture, And Increased Resorption Following Exposure To Ionizing Radiation, Jeffrey Willey
All Dissertations
Skeletal complications from radiation therapy have been described for breast, brain, pelvic, and blood cancers. These problems include atrophy, fractures, and osteoradionecrosis. Improved survivorship rates of cancer patients receiving radiotherapy increases the importance of understanding the causal mechanisms and long-term effects of radiation-induced bone loss. One such long-term effect is bone fractures following radiation therapy for cancer treatment. The incidence of hip fractures is significantly increased following targeted radiotherapy for cancer. This decline in bone health can have a severe impact on the patient's functional capabilities. This damage to bone following irradiation is thought to involve damage to both osteoblasts …
Study Of Electrospinning Of Property-Modulated Biomedical Microfibers, Scott Taylor
Study Of Electrospinning Of Property-Modulated Biomedical Microfibers, Scott Taylor
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Electrospinning technologies have been of great interest in recent years, due in large part to developments in the field of Bioengineering, including the preparation of novel polymers and advances in tissue engineering, specifically for therapeutic applications. The electrospinning process is, in general, a very benign process that allows the preparation, under mild conditions, of micro- and nano-scale fibers from fiberforming materials, as well as traditionally non-fiber forming materials. This process is of specific interest to the biomedical community because it affords an opportunity to create highly-tailored novel constructs with a multitude of possible uses. Some proposed uses for these materials …
Development Of An In Vitro Test System For Breast Cancer Research, Chih-Chao Yang
Development Of An In Vitro Test System For Breast Cancer Research, Chih-Chao Yang
All Dissertations
In the United States, breast cancer is the third most common cause of cancer death (after lung cancer and colon cancer). In 2007, breast cancer is expected to cause 40,910 deaths (7% of cancer deaths; almost 2% of all deaths) in the U.S. The long term goal of this project is to develop an in vitro test system that can be used to develop breast cancer vaccine or screen breast cancer chemotherapy. This dissertation was driven by four objectives and it can be thought as a toolbox that provides practical experimental design and lab work for the development of an …
In Situ Photopolymerized Hydrogels For Enhancing Protein Delivery, Chien-Chi Lin
In Situ Photopolymerized Hydrogels For Enhancing Protein Delivery, Chien-Chi Lin
All Dissertations
In recent years, there has been immense interest in the utilization of photopolymerized hydrogels as carriers for controlled protein delivery and cell scaffolds for tissue engineering applications. Although poly(ethylene glycol) (PEG)-based hydrogels formed from mild photopolymerization methods have been suggested as biocompatible matrices that allow for safely encapsulating biomolecules including proteins, peptides, DNA, and cells, the adverse effects of photopolymerization reactions on the encapsulated proteins have largely been overlooked. In addition, conventional hydrophilic hydrogels fail to effectively control protein delivery rates due to their high permeability. These two problems are critical since the delivery of protein therapeutics from hydrogel matrices …
Differentiation Modulation Of Adult Stem Cells In An Adipose System, Aditya Chaubey
Differentiation Modulation Of Adult Stem Cells In An Adipose System, Aditya Chaubey
All Dissertations
The need for soft tissue reconstruction or augmentation has increased continuously over the years. This need is compounded by patients suffering from post-traumatic repair and congenital soft-tissue deformities. All the current options available to treat the soft tissue deformities have inherent difficulties associated with them. Hence, more research is needed to come up with a better solution to this problem which is only going to increase in magnitude. Tissue engineering is a relatively new technique which has the potential to deliver a cell-based device which can overcome the problems associated with traditional therapies. However, before it becomes clinically viable we …
Activation Of Systemic Adhesion Molecules And C Reactive Protein In Response To Biomaterial Particle Induced Cytokine Production, Robin Graham
All Dissertations
Chronic inflammation has been identified as a major contributor to many diseases including, atherosclerosis, osteoporosis, dementia, Alzheimer's disease and type 2 diabetes, to name a few. Many of the same markers of inflammation found in the diseases listed above, including IL-1, IL-6, TNF-α, NF-B and adhesion molecules, have also been found in wear debris induced osteolysis. While the local response to biomaterial wear debris has been characterized quite extensively, very little is known about the systemic effect of biomaterial wear debris on the inflammatory system. The objectives of this study were to evaluate the in vitro and in vivo inflammatory …
Non-Boltzamann Sampling For The Accurate Calculation Of Peptide-Surface Adsorption Free Energy, Feng Wang
Non-Boltzamann Sampling For The Accurate Calculation Of Peptide-Surface Adsorption Free Energy, Feng Wang
All Dissertations
%Abstract
The objectives of this project are 1) the setup and refinement of peptide-SAM surface model systems with explicit water using CHARMM and 2) the development of up-to-date simulation protocols for the accurate calculation of adsorption free energy by incorporating the recent development of non-Boltzmann sampling methods in molecular dynamics and applying it for the calculation of adsorption free energy of short peptides onto well-characterized self-assembled monolayers, which is important for understanding protein/surface interactions.
A software package called the Simulation Template Engine for Peptides at Surfaces (STEPS) was developed for the fulfillment of the first objective. It facilitates the automatic …
The Differential Effects Of Two Critical Osteoclastogenesis Stimulating Factors On Bone Biomechanics, Yuyu Yuan
The Differential Effects Of Two Critical Osteoclastogenesis Stimulating Factors On Bone Biomechanics, Yuyu Yuan
All Dissertations
Many skeletal diseases, such as osteoporosis and malignant bone metastases, are generally osteolytic and associated with increased bone resorption and decreased bone strength. Within a complex cytokine environment, the proteins RANKL and M-CSF are critical for osteoclast differentiation and activation, and thus fundamental effectors of osteolytic disorders. Previous studies showed that M-CSF stimulates the proliferation and early differentiation of osteoclast progenitors to osteoclast lineage, while RANKL targets the later stages of fusion and activation, and stimulates the formation of functional active osteoclasts. However, impacts of artificially elevated levels of these proteins on the skeleton system have not been fully characterized. …
Spatial Sensors For Quantitative Assessment Of Retrieved Arthroplasty Bearings, Melinda Harman
Spatial Sensors For Quantitative Assessment Of Retrieved Arthroplasty Bearings, Melinda Harman
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Evaluation of retrieved joint arthroplasty bearings provides unique evidence related to the physiological environment in which bearing materials are expected to perform. This dissertation describes the development of novel spatial sensors and measurement strategies for standardized, quantitative assessments of arthroplasty bearings, including total knee replacements, unicompartmental knee replacements, and total hip replacements. The approach is to assess bearings that endured a finite duration of function in patients, with particular emphasis on expanding our understanding of the biomechanical conditions specific to bearing function and wear in the physiological environment. Several quantifiable parameters are identified that prove comparable to pre-clinical in vitro …
Dynamic Measurements Of Lubrication Film Thickness Of Uhmwpe Contacts For Total Joint Replacements, Andrew Clark
Dynamic Measurements Of Lubrication Film Thickness Of Uhmwpe Contacts For Total Joint Replacements, Andrew Clark
All Dissertations
A new contact sensing technology previously developed in the Biotribology Laboratory at Clemson University was further studied, evaluated, and characterized to extend its use to the measurement of lubricating film thickness. First, the laboratory's force-controlled knee joint simulator was used while dynamic contact pressure measurements under both dry and lubricated conditions were made using the sensor technology employed in two different artificial knee implant geometries. Each implant was machined by the manufacturer from custom blocks of ultra high molecular weight polyethylene (UHMWPE) containing a grid of discrete sensing regions. The difference between the dry and lubricated contact areas measured at …
Tribological Systems For The Evaluation Of Supercritical Carbon Dioxide Sterilized Ultra-High Molecular Weight Polyethylene, John Desjardins
Tribological Systems For The Evaluation Of Supercritical Carbon Dioxide Sterilized Ultra-High Molecular Weight Polyethylene, John Desjardins
All Dissertations
Polymeric biomaterials are especially susceptible to changes in mechanical and surface material properties as a result of sterilization processes that involve heat, moisture and/or irradiation. This is problematic for polymeric materials used in total joint replacement bearings, as these constructs must retain their superior mechanical and tribological properties over their expected 10-20 years of implantation. A younger more active patient population has now exacerbated this longevity issue with service lifetimes of 20+ years now being expected. Supercritical phase carbon dioxide (SC-CO2) is an industrial solvent that has shown great promise as a sterilizing agent in the food industry. Recently, SC-CO2 …
Stabilization Of Vascular Elastin By Treatment With Tannins, Jason Isenburg
Stabilization Of Vascular Elastin By Treatment With Tannins, Jason Isenburg
All Dissertations
Elastin is an important component of the extracellular matrix that endows cardiovascular tissue with the ability to extend and recoil repetitively. It has been shown that elastin may be vulnerable to enzymatic degradation and calcification in certain pathological cases. As a result, elastin degeneration may be associated with the failure of tissue-derived cardiovascular devices, such as bioprosthetic heart valves, as well as the development and progression of aortic aneurysms, a disease associated with progressive connective tissue degeneration of the arterial wall. Therefore, an elastin stabilizing agent may be beneficial for both applications. As such, our objectives for elastin stabilization are …