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A Comparative Evaluation Of Antioxidant And Anticancer Activity Of Essential Oil From Origanum Onites (Lamiaceae) And Its Two Major Phenolic Components, Aysun Özkan, Ayşe Erdoğan
A Comparative Evaluation Of Antioxidant And Anticancer Activity Of Essential Oil From Origanum Onites (Lamiaceae) And Its Two Major Phenolic Components, Aysun Özkan, Ayşe Erdoğan
Turkish Journal of Biology
The objectives of the present study were to determine the effects of essential oil from Turkish oregano (Origanum onites L.) and its 2 major phenolic components, carvacrol and thymol, on cell viability, and to investigate their cytoprotective (antioxidant) effects against hydrogen peroxide-induced cytotoxicity and membrane damage in hepatoma G2 (Hep G2) cells. Their antioxidant properties were also evaluated with 2 in vitro complementary test systems: DPPH radical scavenging activity and linoleic acid oxidation inhibition. The aerial parts of the essential oil were isolated using a Clevenger-type apparatus (hydrodistillation) and its components were analyzed by gas chromatography/mass spectrometry (GC/MS). The essential …
Determining Mitotic Index In Peripheral Lymphocytes Of Welders Exposed To Metal Arc Welding Fumes, Ceylan Alakoç, Hali̇l Erhan Eroğlu
Determining Mitotic Index In Peripheral Lymphocytes Of Welders Exposed To Metal Arc Welding Fumes, Ceylan Alakoç, Hali̇l Erhan Eroğlu
Turkish Journal of Biology
Gas metal arc welding is a widely used method in a lot of industrial areas as it is cheap and produces high quality results. In the present study, peripheral blood lymphocytes of 23 welders and 25 non-exposed subjects were monitored for cytotoxicity. Information regarding the subjects' ages, smoking habits, alcohol consumption, duration of exposure, and medicine usage was recorded. According to the results, mitotic index (2.278 ± 1.325) of the welders was higher than that of those non-exposed (0.956 ± 0.616) (P < 0.01). There was no significant difference between mitotic index rates of smoking and non-smoking subjects. In contrast, there were significant differences between non-exposed subjects and welders for both smoking and non-smoking (P < 0.05). It was found that as the time of exposure to welding fumes increased so did the mitotic index rates (P < 0.05). Both a positive correlation in welders and a negative correlation in non-exposed subjects were observed between mitotic index frequency and age. In the light of the results obtained, it is advised that welders who work with gas metal arc welding be instructed and necessary protective measures be taken because of the risk caused by metal arc welding fumes.
Comparative Analysis Of Dna Extracted From Fish Species Preserved In Formalin In Two Different Periods, Manickam Raja, Murugan Muralidharan, Muthukumarasamy Arunachalam
Comparative Analysis Of Dna Extracted From Fish Species Preserved In Formalin In Two Different Periods, Manickam Raja, Murugan Muralidharan, Muthukumarasamy Arunachalam
Turkish Journal of Biology
DNA was extracted from 2 cyprinid fishes kept under formalin preservation in 2 different time durations, and the quality and quantity were analyzed. The tissue samples from specimens preserved for shorter duration yielded higher quantity of DNA compared to long-term preserved tissues. ANOVA (one way) on the quantity of the DNA from the samples of 2 periods showed that the genetic material obtained from the recently preserved samples was highly significant (P > 0.001). The quality of the genetic material irrespective of the period of preservation was found to be the same. The purpose of the study was to check the …
Determination Of Antimicrobial And Antiproliferative Activities Of The Aquatic Moss Fontinalis Antipyretica Hedw., Fi̇li̇z Savaroğlu, Cansu Fi̇li̇k İşçen, Ayşe Pinar Öztopcu Vatan, Selda Kabadere, Semra İlhan, Ruhi̇ Uyar
Determination Of Antimicrobial And Antiproliferative Activities Of The Aquatic Moss Fontinalis Antipyretica Hedw., Fi̇li̇z Savaroğlu, Cansu Fi̇li̇k İşçen, Ayşe Pinar Öztopcu Vatan, Selda Kabadere, Semra İlhan, Ruhi̇ Uyar
Turkish Journal of Biology
The purpose of the current study was to investigate the possible antimicrobial and anticancer properties of the aquatic moss Fontinalis antipyretica Hedw. (Fontinalaceae). We first obtained 8 different extracts (methanol; chloroform; acetone; ethyl acetate A, B, C, and D) by two different extraction processes. The antimicrobial activity of these extracts was then assessed by using the well diffusion method against 8 bacterial and 7 fungal strains. The results of antimicrobial studies showed that extracts of chloroform, acetone, and ethyl acetate A and C were active against almost all the species tested. The most effective extract was C, which was then …
Testing The Putative Effect Of Dicer-Substrate Sirnas In Regulating Gene Expression At Transcriptional Level, Müge Kovancilar, Mehmet Nejat Dalay, Uğur Deli̇gezer
Testing The Putative Effect Of Dicer-Substrate Sirnas In Regulating Gene Expression At Transcriptional Level, Müge Kovancilar, Mehmet Nejat Dalay, Uğur Deli̇gezer
Turkish Journal of Biology
RNA interference (RNAi) pathway is a gene silencing process during which small double-stranded RNA (dsRNA) molecules trigger the degradation of homologous RNA targets. Small interfering RNAs (siRNAs) are the mediators of the RNAi that can be induced in vitro and in vivo by direct application of chemically synthesized siRNAs. Recently, promoter-targeted small non-coding RNAs have been described to be capable of regulating gene expression at the transcriptional level. In the present study we tested the hypothesis that Dicer-substrate siRNAs (D-siRNAs), which trigger gene silencing through intrinsic RNAi pathway and are therefore more potent in gene knockdown than traditionally used 21-23mer …
Changes In The Hemolymph Total Protein Of Galleria Mellonella (Lepidoptera: Pyralidae) After Parasitism And Envenomation By Pimpla Turionellae (Hymenoptera: Ichneumonidae), Olga Sak, Ekrem Ergi̇n, Fevzi̇ Uçkan, David B. Rivers, Ayli̇n Er
Changes In The Hemolymph Total Protein Of Galleria Mellonella (Lepidoptera: Pyralidae) After Parasitism And Envenomation By Pimpla Turionellae (Hymenoptera: Ichneumonidae), Olga Sak, Ekrem Ergi̇n, Fevzi̇ Uçkan, David B. Rivers, Ayli̇n Er
Turkish Journal of Biology
Venom from the endoparasitoid Pimpla turionellae L. (Hymenoptera: Ichneumonidae) contains a mixture of biologically active components, which display potent paralytic, cytotoxic, and cytolytic effects towards hosts. Here, we further investigate if parasitism or envenomation by P. turionellae alters total protein of its host Galleria mellonella L. (Lepidoptera: Pyralidae). Various venom concentrations representing doses previously determined to yield host responses yet fall below the calculated LD99 were used for pupae and larvae. Parasitization was only assayed for host pupa since P. turionellae females normally parasitize host prepupae and pupae in nature. Hemolymph total protein concentration remained relatively steady at all doses …
Phenolic Constituents Of Hypericum Triquetrifolium Turra (Guttiferae) Growing In Turkey: Variation Among Populations And Plant Parts, Cüneyt Çirak, Jolita Radusiene, Valdimaras Janulis, Liudas Ivanauskas, Necdet Çamaş, Ali̇ Kemal Ayan
Phenolic Constituents Of Hypericum Triquetrifolium Turra (Guttiferae) Growing In Turkey: Variation Among Populations And Plant Parts, Cüneyt Çirak, Jolita Radusiene, Valdimaras Janulis, Liudas Ivanauskas, Necdet Çamaş, Ali̇ Kemal Ayan
Turkish Journal of Biology
Hypericum triquetrifolium Turra (Guttiferae) has received considerable scientific interest in recent years, as it is a source of a variety of biologically active compounds including phenolics. The present study was conducted to determine the variation in the content of several phenolics, namely phenylpropane chlorogenic acid and flavonoids such as rutin, hyperoside, apigenin-7-O-glucoside, kaempferol, quercitrin, quercetin and amentoflavone among four H. triquetrifolium populations from the Central Black Sea Region of Turkey. The aerial parts representing a total of 30 shoots were collected at full flowering in each population. After drying at room temperature, they were dissected into floral, leaf and stem …
Numerical Classification Of Streptomycetes Isolated From Karstic Caves In Turkey, Mustafa Yamaç, Kami̇l Işik, Nevzat Şahi̇n
Numerical Classification Of Streptomycetes Isolated From Karstic Caves In Turkey, Mustafa Yamaç, Kami̇l Işik, Nevzat Şahi̇n
Turkish Journal of Biology
In the present study, a total of 290 actinomycetes isolates were obtained from 19 karstic caves in Turkey. Out of these, 131 were chosen for numerical classification studies according to their morphology and pigmentation properties. Representative isolates were compared with the reference Streptomyces strains based on their various taxonomic characteristics such as biochemical, physiological, nutritional, and morphological, totalling 134 unit characters. The data were evaluated using simple matching coefficients (S_{SM}), and clustering achieved using the Complete algorithm by X-Taxon and NTSys-pc programs. The test strains were assigned to 9 major (5-11 strains), 18 minor (2-4 strains), and 31 single membered …
Influence Of Different Sterilization Methods On Callus Initiation And Production Of Pigmented Callus In Arnebia Densiflora Ledeb., Hati̇ce Çölgeçen, Ufuk Koca Çalişkan, Gülnur Toker
Influence Of Different Sterilization Methods On Callus Initiation And Production Of Pigmented Callus In Arnebia Densiflora Ledeb., Hati̇ce Çölgeçen, Ufuk Koca Çalişkan, Gülnur Toker
Turkish Journal of Biology
We analyzed the effects of sodium hypochlorite and Plant Preservative Mixture (PPM) on surface sterilization. We also examined the effects that the addition of an antibiotic-antimycotic solution to the culture medium had on callus induction. Explants were initially sterilized with different concentrations of sodium hypochlorite and cultured on MS media containing kinetin (0.29 \muM) and naphthalene acetic acid (NAA, 10 \muM). No calluses were produced, either because of contamination of the explants, or loss of explants as a result of the high levels of sodium hypochlorite. The application of PPM and antibiotics at different concentrations reduced contamination and led to …
Isolation, Production, And Characterization Of An Extracellular Lipase From Trichoderma Harzianum Isolated From Soil, Serdar Ülker, Arzu Özel, Ahmet Çolak, Şengül Alpay Karaoğlu
Isolation, Production, And Characterization Of An Extracellular Lipase From Trichoderma Harzianum Isolated From Soil, Serdar Ülker, Arzu Özel, Ahmet Çolak, Şengül Alpay Karaoğlu
Turkish Journal of Biology
This is the first report about the characterization of Trichoderma harzianum lipase. A novel strain of Trichoderma harzianum IDM14D was isolated from soil. The isolated strain was cultivated for lipase production in shake flasks at 30 °C for 7 days. For lipase production, it was determined that the best carbon source was glucose and the best nitrogen source was peptone. Maximum biomass was produced at a concentration of 1.25 g/L, in 7 days. The optimum pH and temperature for activity of the enzyme were 8.5 and 40 °C, respectively. The lipase was stable at a pH range of 8.0-10.0 and …
Increased Production Of Coleoptera-Specific Delta-Endotoxin By Homologous Expression Of Cry3aa11 In Bacillus Thuringiensis Mm2, Aslihan Kurt, Gülay Özcengi̇z
Increased Production Of Coleoptera-Specific Delta-Endotoxin By Homologous Expression Of Cry3aa11 In Bacillus Thuringiensis Mm2, Aslihan Kurt, Gülay Özcengi̇z
Turkish Journal of Biology
The cry3Aa11 gene of Bacillus thuringiensis strain Mm2 (B. thuringiensis Mm2) was PCR-amplified and cloned into E. coli/B. thuringiensis shuttle vector pHT315. The recombinant plasmid carrying the cry3Aa11 gene was introduced into the parental B. thuringiensis Mm2. The toxin production capacities of parental and recombinant strains grown in DSM, containing either 50 mM or 200 mM inorganic phosphate (Pi), were compared by SDS-PAGE and western blot analyses. In comparison to the parental strain, recombinant B. thuringiensis Mm2 produced 4-fold more Cry3Aa11 toxin after 12 h of growth in the presence of 50 mM Pi. However, the 200 mM Pi concentration …
Antioxidant Capacity Of Juice From Different Papaya (Carica Papaya L.) Cultivars Grown Under Greenhouse Conditions In Turkey, Aysun Özkan, Hami̇de Gübbük, Esma Güneş, Ayşe Erdoğan
Antioxidant Capacity Of Juice From Different Papaya (Carica Papaya L.) Cultivars Grown Under Greenhouse Conditions In Turkey, Aysun Özkan, Hami̇de Gübbük, Esma Güneş, Ayşe Erdoğan
Turkish Journal of Biology
The fruits of Carica papaya L. (Caricaceae) are valuable as food and are also used in traditional medicine. The present study was designed to assess the antioxidant potential of the juices of 3 papaya cultivars (PCJ): Sunrise Solo, Red Lady, and Tainung. The antioxidant capacity of PCJ obtained from fully ripened fruit was determined by the following methods: scavenging of the free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH), reducing power assay, scavenging of superoxide radicals, 2-deoxyribose oxidation assay, and thiocyanate assay. Total phenolic content (TPC) of PCJ was determined by the Folin-Ciocalteu method. In the DPPH assay (IC_{50} values), the effect of Sunrise …
The Optimization Of Adna Extraction Protocol And Sex Determination Of Bronze Age Individuals From Oylum Höyük (Kilis, Turkey), Serdal Arslan, Ayşen Açikkol, Ertan Mahi̇r Korkmaz
The Optimization Of Adna Extraction Protocol And Sex Determination Of Bronze Age Individuals From Oylum Höyük (Kilis, Turkey), Serdal Arslan, Ayşen Açikkol, Ertan Mahi̇r Korkmaz
Turkish Journal of Biology
Oylum Höyük falls within the borders of the province of Kilis, located in the southern part of Turkey. It is an important Bronze Age settlement dating back to 5500 BC. In this study, the sex of 36 individuals found in Oylum Höyük was examined using morphological and genetic methods. In the morphological method, sex was identified in 14 female and 13 male individuals. The remaining 8 individuals could not be identified due to the poor preservation of their skeletal remains. Genetic sex was determined with the amplification of sex-specific X, Y centromeric alphoid repeats and ZFX-ZYF gene regions of DNA, …
C- And Nor Stained Karyotypes Of Mole Rat, Nannospalax Xanthodon (2n = 54) From Kırıkkale, Turkey, Ati̇lla Arslan, Kubi̇lay Toyran, Serdar Gözütok, Tarkan Yorulmaz
C- And Nor Stained Karyotypes Of Mole Rat, Nannospalax Xanthodon (2n = 54) From Kırıkkale, Turkey, Ati̇lla Arslan, Kubi̇lay Toyran, Serdar Gözütok, Tarkan Yorulmaz
Turkish Journal of Biology
In the present study, the 2n = 54 chromosomal race of blind mole rats, Nannospalax xanthodon superspecies, from Kırıkkale Province in Turkey was investigated. Conventional chromosome staining, Ag-NOR (Nucleolus Organizer Region) staining, and C-banding analysis were carried out on specimens of mole rats. The karyotype including 3 metacentric pairs (nos. 1-3), 3 submetacentric pairs (nos. 4-6), 3 subtelocentric pairs (nos. 7-9), and 17 acrocentric pairs (nos. 10-26) of autosomes (NFa = 70). C-heterochromatin regions were found in the centromeric and pericentromeric region and the short arms of some bi-armed autosomal pairs, and C-heterochromatin was localized in pericentromeric areas of a …
Investigation Of Legionella Pneumophila And Free Living Amoebas In The Domestic Hot Water Systems In İstanbul, Di̇lara Meryem Burak, Zuhal Zeybek
Investigation Of Legionella Pneumophila And Free Living Amoebas In The Domestic Hot Water Systems In İstanbul, Di̇lara Meryem Burak, Zuhal Zeybek
Turkish Journal of Biology
Legionella pneumophila (L. pneumophila), known to cause legionnaires' disease, has been found in various aquatic environments and man-made water systems. Free living amoebas (FLAs) live in the same environments and can cause infections such as encephalitis, keratitis. Moreover, FLAs play a major role in L. pneumophila transmission. In the present study, the presence of L. pneumophila and FLAs was examined in water and swab samples obtained from shower heads (hot water) in 61 houses in İstanbul. Nonnutrient agar (NNA) was used for isolation of FLAs in all samples and buffered charcoal yeast extract (BCYE) agar with addition of glycine, vancomycine, …
Production Of A Novel Bifunctional Catalase-Phenol Oxidase Of Scytalidium Thermophilum In The Presence Of Phenolic Compounds, Yonca Yüzügüllü, Zümrüt Begüm Ögel, Ufuk Bölükbaşi, Nursen Çoruh, Gürkan Karakaş
Production Of A Novel Bifunctional Catalase-Phenol Oxidase Of Scytalidium Thermophilum In The Presence Of Phenolic Compounds, Yonca Yüzügüllü, Zümrüt Begüm Ögel, Ufuk Bölükbaşi, Nursen Çoruh, Gürkan Karakaş
Turkish Journal of Biology
A novel bifunctional catalase with additional phenol oxidase activity (CATPO) is produced by the thermophilic fungus, Scytalidium thermophilum, in a growth-associated manner. In order to study the biological significance of this dual enzyme in relation to phenolic compounds, 14 phenolics were tested for their effect on growth and CATPO production. It was determined that some phenolics exerted a negative effect on growth (catechol, coumaric acid, hydroquinone, kaempferol, myricetin), while others either did not influence growth in a negative manner or enhanced growth by a maximum of 50% increase in biomass (caffeic acid, chlorogenic acid, catechin, gallic acid, resorcinol, vanillic acid). …
Indirect Somatic Embryogenesis And Shoot Organogenesis From Cotyledonary Leaf Segments Of Digitalis Lamarckii Ivan., An Endemic Medicinal Species, Sandeep K. Verma, Bahti̇yar Buhara Yücesan, Songül Gürel, Ekrem Gürel
Indirect Somatic Embryogenesis And Shoot Organogenesis From Cotyledonary Leaf Segments Of Digitalis Lamarckii Ivan., An Endemic Medicinal Species, Sandeep K. Verma, Bahti̇yar Buhara Yücesan, Songül Gürel, Ekrem Gürel
Turkish Journal of Biology
This study describes, for the first time, an in vitro protocol for the development of somatic embryos and shoots from a callus derived from cotyledonary leaf segments excised from 3-week-old in vitro-germinated seedlings of Digitalis lamarckii Ivan. (dwarf foxglove), an endemic medicinal species of Turkey. The embryogenic callus was induced on Murashige and Skoog (MS) medium containing 0.54 \muM \mu-naphthalene acetic acid (NAA) and 2.22 \muM 6-benzylaminopurine (BAP). Somatic embryos developed readily when the embryogenic callus was transferred to MS medium containing BAP (4.44 or 8.87 \muM) alone or BAP combined with NAA (1.34, 2.69, or 5.37 \muM). The most …
Determination And Pathogenicity Of The Bacterial Flora Associated With The Spruce Bark Beetle, Ips Typographus (L.) (Coleoptera: Curculionidae: Scolytinae), Hacer Muratoğlu, Kazim Sezen, Zi̇hni̇ Demi̇rbağ
Determination And Pathogenicity Of The Bacterial Flora Associated With The Spruce Bark Beetle, Ips Typographus (L.) (Coleoptera: Curculionidae: Scolytinae), Hacer Muratoğlu, Kazim Sezen, Zi̇hni̇ Demi̇rbağ
Turkish Journal of Biology
The Eurasian spruce bark beetle, Ips typographus (L.) (Coleoptera: Curculionidae: Scolytinae), is one of the most serious pests of spruce trees. We identified 8 bacterial isolates from this pest using conventional bacteriological tests (API 20E and API 50CH strips, and VITEK system (bioMerieux) analysis) and 16S rRNA gene sequence analysis. Based on these studies, all isolates could be identified to the genus or species level as Bacillus sphaericus (It1), Acinetobacter sp. (It2), Kluyvera cryocrescens (It3), Acinetobacter sp. (It4), Vagococcus sp. (It5), Acinetobacter sp. (It6), Proteus vulgaris (It7), and Serratia liquefaciens (It8). We also evaluated the pathogenicity of these bacteria on …
The Toxicity Of A Synthetic Industrial Detergent And A Corrosion Inhibitor To Brackish Water Fish (Tilapia Guineensis), Dori̇s Fouwe Ogeleka, Lawrence Ikechukwu Ezemonye, Feli̇x E. Okieimen
The Toxicity Of A Synthetic Industrial Detergent And A Corrosion Inhibitor To Brackish Water Fish (Tilapia Guineensis), Dori̇s Fouwe Ogeleka, Lawrence Ikechukwu Ezemonye, Feli̇x E. Okieimen
Turkish Journal of Biology
The lethal effects resulting from incessant release of toxic surfactant-containing industrial chemicals into the Niger Delta Ecological Zone of Nigeria was studied using a laboratory toxicity test. The study employed the Organisation for Economic Development and Cooperation (OECD) 203 protocol. The test organism (Tilapia guineensis [fish]) was obtained from a brackish water environment and exposed to Neatex (industrial detergent) and Norust CR 486 (corrosion inhibitor) at concentrations of 6.25, 12.5, 25, 50, and 100 mg/L for 96 h. The ecological endpoint considered was mortality at 96 h, and the results obtained indicate that concentration and duration of exposure influenced the …
117-124 Chlorococcal Chlorophyte Composition, Community Structure, And Seasonal Variations In The Shallow Lakes Of The Kızılırmak Delta, Turkey, Faruk Maraşlioğlu, Eli̇f Neyran Soylu, Ari̇f Gönülol
117-124 Chlorococcal Chlorophyte Composition, Community Structure, And Seasonal Variations In The Shallow Lakes Of The Kızılırmak Delta, Turkey, Faruk Maraşlioğlu, Eli̇f Neyran Soylu, Ari̇f Gönülol
Turkish Journal of Biology
The chlorococcal chlorophyte community structure and seasonal variations in terms of species composition and abundance of dominant species in Tatlı, Gıcı, Liman, and Cernek lakes (Samsun, Turkey) were investigated between 1996 and 2003. Species richness and diversity (Shannon-Weaver, H^') were measured monthly from stations at the surface of each lake. Changes in the Chlorococcales community structure were examined with nonmetric multidimensional scaling (NMDS) ordinations. A total of 36 Chlorococcales species were identified in these lakes located in the Kızılırmak Delta. While the Chlorococcales composition was similar in Tatlı, Gıcı, and Liman lakes, Cernek Lake showed different seasonal trends, indicating high …
Uv-B Induces Cell Death In The Lichen Physcia Semipinnata (J.F.Gmel), Di̇lek Ünal, Yi̇ği̇t Uyanikgi̇l
Uv-B Induces Cell Death In The Lichen Physcia Semipinnata (J.F.Gmel), Di̇lek Ünal, Yi̇ği̇t Uyanikgi̇l
Turkish Journal of Biology
We have examined the consequences of Ultraviolet-B (UV-B) irradiation in the thallus of the lichen Physcia semipinnata (J.F.Gmel) in terms of cell viability and apoptotic-like formation. UV-B induced oligonucleosomal DNA fragmentation was detected by TUNEL assay and this is the first study showing DNA fragmentation in thalli. The intensity of TUNEL-positive cells after exposure to UV-B at doses up to 95.9 J/cm2 was higher in the photobiont layer than the mycobiont layer.
Isolation And Identification Of Trichoderma Species From Different Habitats And Their Use For Bioconversion Of Solid Waste, Ahsanur Rahman, Most. Ferdousi Begum, Matiur Rahman, M. A. Bari, G.N.M. Illias, M. Firoz Alam
Isolation And Identification Of Trichoderma Species From Different Habitats And Their Use For Bioconversion Of Solid Waste, Ahsanur Rahman, Most. Ferdousi Begum, Matiur Rahman, M. A. Bari, G.N.M. Illias, M. Firoz Alam
Turkish Journal of Biology
The native population of Trichoderma species collected from different habitats was quantified and characterised. The identified strains are T. harzianum (IMI-392432, 392433, 392434); T. pseudokoningii (IMI-392431) and T. virens (IMI-392430). Out of five strains, T. harzianum was the most common in all of the habitats. Colony forming units (cfu's) of Trichoderma species varied significantly (P = 0.05) in different habitats and were positively correlated with the physico-chemical characteristics of the habitat. Trichoderma was found to have adapted to an acidic habitat. In testing for an efficient bioconversion agent, T. harzianum (IMI-392432) was found to be the most effective in kitchen …
The Antioxidant And Dna Protection Potential Of Indian Tribal Medicinal Plants, Gunjan Guha, Venkatadri Rajkumar, Lazar Mathew, Rangasamy Ashok Kumar
The Antioxidant And Dna Protection Potential Of Indian Tribal Medicinal Plants, Gunjan Guha, Venkatadri Rajkumar, Lazar Mathew, Rangasamy Ashok Kumar
Turkish Journal of Biology
Prevention and treatment of various degenerative diseases using traditional medicines is increasingly generating interest, especially in geriatric clinical research. This study aimed to evaluate the free-radical scavenging properties and potential to prevent DNA damage (from oxidative stress) of 56 extracts (polar and non-polar) from 14 medicinal plants (from 12 families) used by diverse Indian tribes and ethnic groups. Although widely known for traditional uses, these plants have not been extensively used for clinical purposes. Scavenging of DPPH^{\bullet} radical by all the extracts was evaluated to estimate their antioxidant potential and rank them accordingly. Extracts occupying the top 10 ranks were …
Isolation And Characterization Of Xylanolytic New Strains Of Anoxybacillus From Some Hot Springs In Turkey, Kadri̇ye İnan, Sabri̇ye Çanakçi, Ali̇ Osman Beldüz
Isolation And Characterization Of Xylanolytic New Strains Of Anoxybacillus From Some Hot Springs In Turkey, Kadri̇ye İnan, Sabri̇ye Çanakçi, Ali̇ Osman Beldüz
Turkish Journal of Biology
Some hot springs located in the west of Turkey were investigated regarding the presence of xylanolytic thermophilic microorganisms. Based on phenotyping characteristics and 16S rRNA gene sequence analysis, 9 of these xylanolytic isolates belonged to the genus Anoxybacillus, and grew optimally at about 50-60 °C on nutrient agar. The 16S rRNA gene sequence analysis showed that these isolates resembled the Anoxybacillus species =>97 and these isolates are members of the genus Anoxybacillus. Based on the DNA-DNA hybridization study, SDS-PAGE profile, and biochemical and physiological features, I3, CT1Sari, and BT2.1 isolates are new strains of Anoxybacillus gonensis; I4.2 and B9.3 …
The Effects Of Leptin Hormone On Locomotor Activity In Syrian Hamsters (Mesocricetus Auratus), Bülent Gündüz, Alper Karakaş
The Effects Of Leptin Hormone On Locomotor Activity In Syrian Hamsters (Mesocricetus Auratus), Bülent Gündüz, Alper Karakaş
Turkish Journal of Biology
The suprachiasmatic nucleus (SCN) generates and controls the circadian rhythms in mammals including the rhythm of locomotor activity. Leptin is a hormone secreted by adipose tissue that informs the brain about the fat stores. SCN neurons express leptin receptors. Here we investigated the effects of 3 different leptin administrations on the locomotor activity of the Syrian hamsters maintained in constant darkness. Animals were intraperitoneally (ip) injected (4 µg/kg), subcutaneously (sc) infused (4 µg/kg), or intra-SCN infused (0.4 µg/kg) with leptin for 3 days at circadian time 10 whereas the controls received saline (0.9% NaCl) at the same time in order …
Is The Induction Of H_2o_2-Detoxifying Antioxidant Enzyme Activities Sufficient To Protect Barley Cultivars From Oxidative Stress By Uv-B Irradiation Alone Or Pretreatment With High Temperature And Nacl?, Hüsnü Çakirlar, Nuran Çi̇çek, Yasemi̇n Ekmekçi̇
Is The Induction Of H_2o_2-Detoxifying Antioxidant Enzyme Activities Sufficient To Protect Barley Cultivars From Oxidative Stress By Uv-B Irradiation Alone Or Pretreatment With High Temperature And Nacl?, Hüsnü Çakirlar, Nuran Çi̇çek, Yasemi̇n Ekmekçi̇
Turkish Journal of Biology
Ultraviolet B (UV-B) irradiation has an adverse effect on plant cells because it causes the generation of reactive oxygen species (ROS). This is scavenged by some endogenous defense systems. In this study, the activities of H_2O_2-detoxifying antioxidant enzymes in 4 barley cultivars pretreated with high temperature or NaCl before UV-B irradiation were investigated. The stress response in the seedlings was determined by measuring the photosynthetic performance index (PI), which significantly decreased in all barley cultivars under almost all treatments compared to their controls. Activities of 3 H_2O_2-detoxifying antioxidant enzymes (ascorbate peroxidase, glutathione reductase, and peroxidase) significantly increased as a result …
Preliminary Studies On Antiinflammatory, Antipyretic, And Antidiarrhoeal Properties Of Evolvulus Alsinoides, U.M.Dhana Lekshmi, P.Neelakanta Reddy
Preliminary Studies On Antiinflammatory, Antipyretic, And Antidiarrhoeal Properties Of Evolvulus Alsinoides, U.M.Dhana Lekshmi, P.Neelakanta Reddy
Turkish Journal of Biology
The ethanolic extract of the whole plant of Evolvulus alsinoides L. (Convolvulaceae) was investigated for antiinflammatory, antipyretic and antidiarrhoeal properties in female albino rats. Preliminary phytochemical studies were carried out to confirm the presence of active chemical constituents in the plant extract, and the result showed a positive report for the presence of flavonoids, alkaloids and cardiac glycosides. The pharmacological result showed that the ethanolic extract at a dose of 250 mg/kg body weight and 500 mg/kg body weight caused a significant (P < 0.05) inhibition of the carrageenan-induced rat paw oedema and a significant (P < 0.05) reduction of hyperpyrexia induced by Brewer's yeast in rats. The extract also elicited marked antidiarrhoeal activity against castor oil-induced diarrhoea at a dose of 500 mg/kg. This study has proved scientifically the ancient claim that the whole plant of Evolvulus alsinoides has antiinflammatory, antipyretic and antidiarrhoeal activities.
Significance Of Absorption Spectra For The Chemotaxonomic Characterization Of Pigmented Bacteria, Nuretti̇n Şahi̇n
Significance Of Absorption Spectra For The Chemotaxonomic Characterization Of Pigmented Bacteria, Nuretti̇n Şahi̇n
Turkish Journal of Biology
A method for characterizing pigmented bacteria based on the numerical analysis of their carotenoid absorption spectra was examined. To achieve this, some non-phototrophic pigmented bacteria that were isolated from various environments and closely related types of species were used. Bacterial carotenoid pigments were extracted with an acetone:methanol (7:2) mixture and the absorption spectra were determined. Data were normalized, and their first derivatives were analyzed using Ward clustering algorithms. Based on the spectral data, isolates were separated into 7 groups. Characterization based on derivative pigment spectral data showed good agreement statistically with biochemical characterization results. The Mantel test indicated that the …
Total Phenolic Contents And Antioxidant Activities Of Prangos Lindl. (Umbelliferae) Species Growing In Konya Province (Turkey), Jehad Ahmed, Ayşegül Güvenç, Nurgün Küçükboyaci, Ayşe Baldemi̇r, Maksut Coşkun
Total Phenolic Contents And Antioxidant Activities Of Prangos Lindl. (Umbelliferae) Species Growing In Konya Province (Turkey), Jehad Ahmed, Ayşegül Güvenç, Nurgün Küçükboyaci, Ayşe Baldemi̇r, Maksut Coşkun
Turkish Journal of Biology
Prangos species are widely used as medicinal plants in Turkey, and 14 species of this genus grow naturally in Anatolia. In the present study, the phenolic contents and antioxidant activities of the water and methanol (MeOH) extracts obtained from the root, herb, and fruits of 4 species of Prangos (Prangos ferulacea, P. heyniae, P. meliocarpoides var. meliocarpoides, and P. uechtritzii) collected in Konya province were compared. The phenolic contents of the samples were determined using Folin-Ciocalteu's phenol reagent. Antioxidant activities of the extracts were studied by qualitative DPPH^. (1,1-diphenyl-2-picrylhydrazyl radical) assay to detect the free radical scavenging activity and by …
Evaluation Of The Antioxidant And Acetylcholinesterase Inhibitory Activities Of Arnebia Densiflora Ledeb., İlkay Orhan, F. Sezer Şenol, Ufuk Koca, Tuğba Erçeti̇n, Gülnur Toker
Evaluation Of The Antioxidant And Acetylcholinesterase Inhibitory Activities Of Arnebia Densiflora Ledeb., İlkay Orhan, F. Sezer Şenol, Ufuk Koca, Tuğba Erçeti̇n, Gülnur Toker
Turkish Journal of Biology
Arnebia densiflora Ledeb. (Boraginaceae), a medicinal plant growing in Turkey, has been reported to contain a well-known red pigment, shikonin, a naphthoquinone derivative. In the current study, the acetylcholinesterase (AChE) inhibitory and antioxidant activities of the chloroform, ethyl acetate, methanol, and water extracts of the root, stem, and flowers of the plant, as well as shikonin, were investigated. AChE inhibition was tested using an ELISA microplate reader at 250, 500, 1000, and 2000 µg mL^{-1}. Antioxidant activity was determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging test and the Fe+2-ferrozine test system for metal chelating power. The results indicated that the …