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Experimental Population Dynamics Of Amyloodiniumocellatum In The Spotted Seatrout, Cynoscion Nebulosus, And The Red Snapper, Lutjanus Campechanus, Ignacio Masson

Dissertations

Amyloodinium ocellatum is a parasitic dinoflagellate that infects warm water marine bony fishes and causes high mortalities in aquaculture settings. It has three life history stages: the feeding trophont, the reproductive tomont, and the infective dinospore. This dissertation describes the characteristics of A. ocellatum infections in juvenile spotted seatrout, Cynoscion nebulosus, and red snapper, Lutjanus campechanus, studies the survival and fecundities of the stages in the life cycle of the parasite and presents a population model for A. ocellatum.

At 25°C and 33 ppt, the peak of trophont detachment occurred on day 2 postinfection in spotted seatrout and day 3 …

Measuring The Impact Of Varied Instructional Approaches In An Introductory Animal Science Course, Brian Bolt

All Dissertations

The objectives of this project were to: evaluate the impact of demographic descriptors (gender, class rank and final grade) on student's self perceived level of engagement in classroom activities; measure the impact of varying teaching styles on a student's likelihood of correctly answering a knowledge based question and: assessing the relationship of knowledge acquisition with their level of engagement. Data were collected on students in the AVS 150, introductory animal science class (n=155) at Clemson University during the fall of 2008. Ten to fifteen minutes of class time were classified as conforming exclusively to one of three types of material …

Investigating The Maintenance Of The Lyme Disease Pathogen, Borrelia Burgdorferi, And Its Vector, Ixodes Scapularis, In Tennessee, Michelle Erin Rosen

Masters Theses

Lyme disease (LD), caused by the bacterium Borrelia burgdorferi and transmitted by blacklegged ticks (Ixodes scapularis), is the most commonly reported vector-borne disease in the United States. Lyme disease is endemic in northeastern states, whereas southern states report far fewer cases. This research evaluated the potential LD health risk to humans associated with blacklegged ticks in Tennessee.

I surveyed 1,018 hunter-harvested deer from 71 counties in fall 2007 and fall 2008. Of these, 160 (15.7%) from 35 counties were infested with I. scapularis — 30 of the counties were new distributional records for this species.

I also evaluated …

Population Ecology And Reproductive Biology Of The Diamondback Watersnake, Nerodia Rhombifer (Serpentes: Colubridae), In Southernmost Texas, Ruben D. Zamora

Theses and Dissertations - UTB/UTPA

Nerodia rhombifer is a polytypic, semi-aquatic snake with a broad geographical distribution ranging from the American Midwest southward to Chiapas, Mexico. Although relatively abundant throughout much of its range, few ecological studies of the species have been conducted. This study provides basic population ecology information in a subtropical habitat. Population data were obtained in a mark-recapture study at Santa Ana National Wildlife Refuge, Hidalgo County, Texas, from August 1995 to December 1998. Specimens taken elsewhere in Hidalgo County provided information on the reproductive biology. This study provides the first absolute density estimates from anywhere within the species’ range. Quantitative information …

Predictive Modeling Of Sulfur Flower Buckwheat (Erigonum Umbellatum Torrey) Using Non-Parametric Multiplicative Regression Analysis, David B. Davis

Theses and Dissertations

Impacts of humans on ecosystems in western United States have necessitated ecological restoration, which includes the development of native seed that can be used for revegetation efforts. Development of such seed sources are costly and time consuming. This study describes the use of non-parametric multiplicative regression analysis (NPMR) to develop a predictive model for occurrence of sulfur-flower buckwheat (Eriogonum umbellatum Torrey) population seed collection. This perennial forb species is of interest for seed source development in the western United States. Presence and absence data for E. umbellatum was taken from the Utah Division of Wildlife Resources Big Game Range …

Impact Of Dietary Arginine On Immunity In Broiler Chicks, Jennifer Lynn D'Amato

Master's Theses

Arginine (ARG) is an immunologic modulator due in part to its role as a substrate for leukocytes. Therefore, the objective of these studies was to evaluate the impact of dietary ARG on ARG utilization in peripheral blood mononuclear cells (PBMC) and thymocytes in broiler chicks as they provide a model that is not confounded by de novo ARG synthesis. Several experiments were performed to measure: 1) ARG transporters as markers of ARG utilization by leukocytes; 2) thymocyte proliferation; 3) PBMC phenotype, and 4) ARG’s impact on the acute phase response (APR). In experiment 1, chicks were fed diets containing 1.20% …

Seasonal Home Range Sizes, Transboundary Movements And Conservation Of Elephants In Northern Tanzania, Alfred P. Kikoti

Open Access Dissertations

Although the unprotected lands of northern Tanzania support large numbers of elephants, and provide critical linkages for wildlife movements across the region, there is little information on the dispersal patterns of elephants in these unprotected lands. Our home range measures (100% MCP) of 21 elephants with satellite collars in four study regions were highly variable (191 to 3,698 km2). Home range sizes (95% fixed kernel) of bulls were typically larger than those of females, and wet season ranges were typically larger than dry season ranges. There were large differences in average home range sizes reflected varying strategies for obtaining food …

Impact Of Weed Management Practices On Grapevine Growth, Yield Components, Plant And Arthropod Abundance, And Carabid Seed Predation In Paso Robles Vineyard, Paolo Sanguankeo

Master's Theses

In the Central Coast of California, USA, wine grape growers are making efforts to identify weed control practices that promote biodiversity in their vineyards while maintaining yields. A field study was conducted in Paso Robles, CA in 2006 and 2007 evaluating the effect on Zinfandel grape-vine growth and production, groundcover plant, and ground dwelling arthropod communities of five weed control practices: 1) flumioxazin, 2) simazine, 3) cultivation, 4) cover crop, and 5) untreated control.

The herbicide treatments had the lowest weed biomass followed by the cultivation, being approximately 10 and 2 times lower than the weed biomass of either the …

Identification And Manipulation Of Resistance To Tomato Spotted Wilt Virus Derived From Solanum Peruvianum, Luis F. Gordillo Jr.

Theses and Dissertations

The domesticated tomato Solanum lycopersicum (L.), formerly known as Lycopersicon esculentum is a genetically well-studied crop species with high-density linkage and molecular maps based on crosses done between cultivated tomato and its distant related wild species. Wild tomato species harbor a wealth of resistance to many pathogens that have been introgressed into domesticated tomato for genetic control of diseases and pests and for improvement of many agronomic traits. The wild tomato S. peruvianum (L.) is the source of the Sw-5 gene, characterized and mapped to chromosome 9 of the tomato genome and introgressed into elite tomato germplasm, providing resistance to …

Mapping The Dorsal Skin Pigmentation Patterns Of Two Sympatric Populations Of Ambystomatid Salamanders, Ambystoma Opacum And A. Maculatum From Northeast Tennessee., Lok Raj Pokhrel

Electronic Theses and Dissertations

Because of growing concern of habitat fragmentation and its adverse effects on salamander communities in Appalachian region, sympatric populations of ambystomatid salamanders A. opacum and A. maculatum were studied in Northeast Tennessee to address a number of questions: i) the extent of sexual size dimorphism (SSD) in both species, ii) what traits influence the dorsal skin pigmentation and how, iii) whether gender differences in developmental stability occur, and iv) the extent of phenotypic variation within each species. The findings of this study revealed SSD in both species of salamanders. The most parsimonious statistical model was developed that explained the influence …

Stable Carbon Isotope Evidence Of Ancient Maya Agriculture At Tikal, Guatemala, Richard Lee Burnett

Theses and Dissertations

Stable carbon isotope analyses of the humin fraction of the soil organic matter were conducted on more than 160 soil profiles from Tikal, Guatemala. The profiles were collected from near areas associated with the earthworks of Tikal; an ancient ditch and parapet construction hypothesized to have formed ancient boundaries of the polity. In addition to the isotope analyses, the physical and chemical characteristics of the horizons were determined. Maize, a C4 plant, formed an integral part of the ancient Maya diet and is the only known C4 plant cultivated by the Maya. Prior to and subsequent to the ancient Maya …

Deer Forage Available Following Silvicultural Treatments In Upland Hardwood Forests And Warm-Season Plantings, Marcus Alan Lashley

Masters Theses

Thinning, herbicide release, and prescribed fire have been used to increase forage availability in pine forests for white-tailed deer, rivaling that available in warm-season food plots. Related data are lacking for hardwood forests. I measured forage availability following 7 silvicultural treatments, including controls (C), forest regeneration methods, and Timber Stand Improvement practices in 4 upland mixed hardwood stands, July–September 2007 and 2008. I also measured forage availability in 4 paired warm-season food plots, including soybeans, lablab, and iron-andclay cowpeas, July–September 2007, and three varieties of soybeans, July–October 2008. I compared nutritional carrying capacity (NCC) of selected species and species from …

Effects Of Syndyphalin-33 On Appetite, Endocrine, And Immune Parameters In The Recently Weaned Pig, Sarah Jo Jenkins

Masters Theses

This thesis discusses the background information regarding the physiological effects the pig encounters during weaning as well as a potential factor that can be used to assist the pig during this time. Specifically, the research focus is to assess the ability of the tri-peptide opioid agonist, Syndyphalin-33 (SD-33), to increase feed intake and body weight and modulate immune responses during the post-weaning period. The results of this research have demonstrated that SD-33 increases feed intake, transiently increases growth hormone and cortisol levels, and increases total white blood cell counts while selectively increasing monocyte numbers in healthy weaned pigs. This research …

Effects Of Weaning And Syndyphalin-33 On Appetite Regulators In Swine, Tabatha Anne Cooper

Masters Theses

The synthetic met-enkephalin syndyphalin-33 (SD-33) increases feed intak in sheep and recently-weaned pigs. An experiment focused on changes in hypothalamic levels of mRNA transcripts from the following genes: μ-opioid receptor (MOR), neuropeptide Y (NPY), orexin (hypocretin; HCRT), melanocortin 4 receptor (MC4R), and agouti-related protein (AGRP). There was no effect of treatment on BW or FI at any point in the study. There is a strong positive correlation (p <0.001) between MOR and MC4 that remains regardless of treatment or time. Weaning resulted in a numerical increase in hypothalamic MOR mRNA expression. There was a treatment effect of MOR expression levels 4 days postweaning. Hypothalamic NPY expression was unchanged through time or treatment. In …

Population And Genetic Impacts Of A 4-Lane Highway On Black Bears In Eastern North Carolina, Jeremy Michael Nicholson

Masters Theses

A 19.3-km section of U.S. Highway 64 in Washington County, North Carolina was rerouted to a 4-lane, divided highway with 3 wildlife underpasses during 2001–2005. I determined the short-term population and genetic impacts of the new highway on American black bears (Ursus americanus). I used DNA from hair samples collected during 7 weekly sampling periods within the project area of the new highway and a nearby control area during 2000 (pre-construction phase) and 2006 (post-construction phase; n = 70 sites for each study area). DNA from the hair samples was used to obtain genotypes of sampled bears using …

Muskrat-River Otter Interactions In And Adjacent To Mammoth Cave National Park, Kentucky, Ryan H. Williamson

Masters Theses

Anecdotal observations have suggested that muskrat (Ondatra zibethicus) populations were dramatically reduced in streams where the North American river otters (Lontra canadensis) were reintroduced. Muskrats predate upon freshwater mussels and it was speculated that river otter reintroduction could result in increased mussel numbers. My objectives were to evaluate the ecological relationship between otter, muskrat, and mussels on the Green and Nolin rivers in Mammoth Cave National Park (MCNP). Seventeen river otters were captured in or relocated to MCNP from January to May 2007. The augmentation was only marginally successful with 3 male river otters establishing home …

Physiological Assessment Of Chenopodium Quinoa To Salt Stress, Arturo Jason Morales

Theses and Dissertations

The physiological responses to salt stress were measured in Chenopodium quinoa. In a greenhouse experiment, salt water was applied to the quinoa varieties, Chipaya and KU-2, and to the model halophyte Thellungiella halophila to assess their relative responses to salt stress. Height and weight data from a seven-week time course demonstrated that both cultivars exhibited greater tolerance to salt than T. halophila. In a growth chamber experiment, three quinoa cultivars, Chipaya, Ollague, and CICA 17 were hydroponically grown and physiological responses were measured with four salt treatments. Tissues collected from the growth chamber treatments were used to obtain …

The Grass Seed Pathogen Pyrenophora Semeniperda As A Biocontrol Agent For Annual Brome Grasses, Thomas E. Stewart

Theses and Dissertations

Bromus tectorum and other annual brome grasses have invaded many ecosystems of the western United States, and because of an annual-grass influenced alteration of the natural fire cycle on arid western range lands near monocultures are created and conditions in which the native vegetation cannot compete are established. Each year thousands of hectares become near monocultures of annual brome grasses. Pyrenophora semeniperda, a generalist seed pathogen of annual grasses, shows major potential as a possible mycoherbicide that could help in reducing the monocultures created by annual grasses. The purpose of this research was to identify the requirements for isolating …

Fish Assemblages In Manistee River Tributaries: Longitudinal Distribution Analysis, Seasonal Variation, And Riparian Improvement Evaluation, Nicholas J. Gressick

Masters Theses

Sedimentation affects both stream physical and biological integrity. Improperly designed stream passage accompanied with sedimentation and altered hydrology can impede fish passage and reduce fish assemblage integrity. The purpose of this study was to: 1) quantify impacts of poorly constructed road stream crossings and eroding banks on fish assemblages, and 2) assess these sites as sediment sources and connectivity breaks on entire fish assemblages and individual fish species. Electrofishing was conducted during spring and fall 2004 and 2005. A total of 29 electrofishing reaches were sampled which included 5 road-stream and streambank restoration sites. Sickle Creek (1st order) had reduced …

Viability Of Alternative Genetic Improvement Strategies Using Whole Genome Selection On Commercial Dairy Operations, Levi W.M. Gassaway

Master's Theses

The objective of this thesis was to determine the viability of alternative genetic improvement strategies (GIS). Each alternative GIS combined the use of whole genome selection (WGS) with common reproductive methods (non-sexed semen artificial insemination (AI), sexed semen AI, embryo transfer utilizing non-sexed semen AI) that can be found on a commercial dairy operation. The viability of each GIS was determined using a discounted gene flow model, designed with parameters of a typical western dairy operation, to evaluate the following variables: reproductive method, selection intensity, accuracy of prediction and female age-class. Of the GIS investigated, a heifer-based strategy that used …

Analysis And Classification Of Sounds Produced By Asian Elephants (Elephas Maximus), Sharon Stuart Glaeser

Dissertations and Theses

Relatively little is known about the vocal repertoire of Asian elephants (Elephas maximus), and a categorization of basic call types and modifications of these call types by quantitative acoustic parameters is needed to examine acoustic variability within and among call types, to examine individuality, to determine communicative function of calls via playback, to compare species and populations, and to develop rigorous call recognition algorithms for monitoring populations.

This study defines an acoustic repertoire of Asian elephants based on acoustic parameters, compares repertoire usage among groups and individuals, and validates structural distinction among call types through comparison of manual and automated …

Spatial Ecology, Population Structure, And Conservation Of The Wood Turtle, Glyptemys Insculpta, In Central New England, Michael T. Jones

Open Access Dissertations

Abstract (Summary) Wood turtles ( Glyptemys insculpta ) are of conservation interest rangewide. Anecdotal accounts demonstrate that some populations have been decimated since 1850, and recent studies demonstrate that declines are still underway. From 2004-2008 I investigated the ecology of wood turtles in Massachusetts and New Hampshire. I obtained between one and five years of annual home range data for 150 turtles, and evaluated population structure at 31 sites in five major watersheds. Seasonal floods displaced 7% of wood turtles annually in one watershed, and accounted for elevated mortality. Twelve wood turtles were displaced < 16.8 km, and two were displaced over a 65-foot dam. Several turtles overwintered at their displacement site and two returned successfully, indicating that floods are a mechanism of population connectivity. Several homing turtles ended up in new areas. Turtles occupied stream segments with gradient < 1%, lower than generally available. Agricultural machinery accounted for most observed mortality, followed by automobiles and mammals. Female turtles exhibit smaller home ranges in agricultural areas. Older turtles move farther from the river than do young turtles, possibly reflecting their familiarity with a former landscape. Population density ranged from 0-40.4 turtles/river-kilometer. The highest densities occur in central New Hampshire and lower densities occur in the Housatonic watershed. Population density is negatively correlated with agriculture at both riparian and watershed scales, and responds unimodally to forest cover. Wood turtle populations in western Massachusetts are declining by 6.6-11.2% annually. I estimated ages of turtles by assessing shell-wear rates from photographs. Wood turtles regularly achieve ages over 80 years, and like related species, do not exhibit clear signs of senescence. Old wood turtles are reproductively dominant, and their survival rates are twice as high as young turtles. Carapace scutes appear to require 80 years to become worn. Population modeling indicates that wood turtle populations are declining in New England due to anthropogenic and natural factors. Conservation efforts must address the effects of agriculture on adult survival. Climate change may negatively affect northeastern wood turtles through increased flooding. Populations in mountainous areas may be likely candidates for conservation because they don't occupy prime agricultural land, but may be more susceptible to floods.

Yolk Androgens And Development In American Kestrel Nestlings, Melanie Dawn Berg

Boise State University Theses and Dissertations

Maternally derived yolk androgens affect many aspects of phenotypic development. To explore this phenomenon I injected clutches of American kestrels (Falco sparverius) with one of three different dosages of testosterone + androstenedione or with vehicle. Morphological measurements taken from nestlings 26 days after hatching showed no significant treatment effect. In 2007, nestlings with more yolk androgens had lower hatching mass and higher hematocrit levels 15 days after hatching than nestlings with less yolk androgens, but the effects disappeared by day 26. In 2008, there were no effects of androgens on mass or hematocrit. Because the results were not …

Metabolic Tracer Studies Of Linoleic And Linolenic Acids To Identify Biohydrogenation Intermediates Produced By Ruminal Microorganisms, Yong-Jae Lee

All Dissertations

Conjugated linoleic acids (CLA) have been shown to regulate many physiological functions affecting human health. These CLA can endogenously originate from trans fatty acids through the enzymatic activity of desaturase, or they are produced from dietary polyunsaturated fatty acids via the biohydrogenation process in the rumen. A minimum of seventeen CLA isomers have been identified in the intestinal contents of ruminants; however, the entire synthetic pathway for all isomers is not known. The objective of this study was to establish the number and identity of intermediates including CLA that originate from linoleic and linolenic acid biohydrogenation. To accomplish this objective, …

Maintaining Population Persistence In The Face Of An Extremely Altered Hydrograph: Implications For Three Sensitive Fishes In A Tributary Of The Green River, Utah, Jared L. Bottcher

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The ability of an organism to disperse to suitable habitats, especially in modified and fragmented systems, determines individual fitness and overall population viability. The bluehead sucker (Catostomus discobolus), flannelmouth sucker (Catostomus latipinnis), and roundtail chub (Gila robusta) are three species native to the upper Colorado River Basin that now occupy only 50% of their historic range. Despite these distributional declines, populations of all three species are present in the San Rafael River, a highly regulated tributary of the Green River, Utah, providing an opportunity for research. Our goal was to determine the timing and …

Design And Evaluation Of Oligonucleotide Microarrays For The Detection Of Bovine Pathogens, Ryan Weldon Black

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Two microarray designs were developed and produced to screen for multiple bovine pathogens commonly found in the cattle industry today. The first microarray was designed, built, and processed in-house using conventional material and equipment and targeted Pasteurella multocida, Manheimia haemolytica, Histophilus somni, and Arcanobacterium pyogenes. For each pathogen, 12 perfect-match oligonucleotide probes, which were also designed in-house, targeted different sections of the respective 16S ribosomal genes, and were coupled with 12 corresponding mismatched probes for background. These arrays were able to produce distinct hybridization patterns for each pathogen that were easily visible without the need for …

Effects Of Direct And Indirect Predator Cues On Heteromyid Seed Selection And Seed Fate, Kelly J. Sivy

Undergraduate Honors Capstone Projects

Many factors affect foraging behavior of rodents, including predation risk, which is thought to influence seed selection and seed handling by desert rodents in patchy environments. Understanding forces that drive seed selection and seed fate can aid understanding of rodents' impacts on vegetation structure and dynamics. In a feeding arena study, we tested how indirect and direct predation cues influence seed selection and handling behaviors (e.g., scatterhoarding and larderhoarding) of two heteromyid rodents, Dipodomys ordii (Ord's kangaroo rat) and Perognathus parvus (Great Basin pocket mouse), foraging on three seed species. The indirect cue was shrub cover: one half of the …

The Effects Of Wastewater Effluent On A Fish And Amphibian Species, Anthony Sowers

All Dissertations

Wastewater effluents have been shown to contain a variety of anthropogenic compounds, many of which have endocrine-disrupting properties. While multiple laboratory studies have shown the effects of such compounds on an individual basis at elevated concentrations, little research has attempted to characterize the effects of exposure to environmentally relevant mixtures of endocrine disruptors. The current study examined the effects of long-term exposure to graded concentrations of wastewater effluent on the fathead minnow, Pimephales promelas, and the northern leopard frog, Rana pipiens. Fathead minnows were exposed from the larval stage through sexual maturity, while northern leopard frogs were exposed as eggs …

The Effects Of Dolphin Education Programs On Visitors' Conservation-Related Knowledge, Attitude And Behavior, Lance Joseph Miller

Dissertations

Zoological institutions typically exhibit dolphins in educational programs such as dolphin shows and interaction programs. The goal of these programs is to entertain visitors while increasing their conservation-related knowledge, attitude and behavior towards dolphins and the marine environment. The purpose of the current study was to examine dolphin shows and interaction programs in terms of their effectiveness in meeting these goals. A multi-institutional study was conducted at six different facilities throughout the United States. A repeated measures design was used to examine the knowledge, attitude and behavior of visitors before, immediately after and three months following participation in dolphin shows …

Greater Sage-Grouse Ecology, Chick Survival, And Population Dynamics, Parker Mountain, Utah, David K. Dahlgren

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

We estimated survival of ~ 1-day-old chicks to 42 days based on radio-marked individuals for the Parker Mountain greater sage-grouse (Centrocercus urophasianus) population. Chick survival was relatively high (low estimate of 0.41 and high estimate of 0.50) compared to other studies. Brood-mixing occurred for 21 % of radio-marked chicks, and within 43 % of radio-marked broods. Our study showed that brood-mixing may be an important ecological strategy for sage-grouse, because chicks that brood-mixed experienced higher survival. Additionally, modeling of chick survival suggested that arthropod abundance is important during the early brood-rearing period (1 - 21 days). We also …