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Genetic Dissection Of Triterpenoid Saponin Production In Chenopodium Quinoa Using Microarray Analysis, Derrick James Reynolds
Genetic Dissection Of Triterpenoid Saponin Production In Chenopodium Quinoa Using Microarray Analysis, Derrick James Reynolds
Theses and Dissertations
Quinoa (Chenopodium quinoa Willd.) is an important food crop for subsistence farmers in the Altiplano (high plains) of Peru, Bolivia, and Argentina. Saponins are part of a diverse family of secondary metabolites that are found in high concentrations in the pericarp of many varieties of quinoa. Due to their bitter taste and anti-nutritive properties, saponins must be removed before the quinoa grain is consumed. There are ‘sweet’ varieties of quinoa that have significantly reduced levels of saponin. Previous research suggests saponin production is controlled by a single locus. The major objective of this research was to elucidate the genetic …
Design And Evaluation Of Oligonucleotide Microarrays For The Detection Of Bovine Pathogens, Ryan Weldon Black
Design And Evaluation Of Oligonucleotide Microarrays For The Detection Of Bovine Pathogens, Ryan Weldon Black
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Two microarray designs were developed and produced to screen for multiple bovine pathogens commonly found in the cattle industry today. The first microarray was designed, built, and processed in-house using conventional material and equipment and targeted Pasteurella multocida, Manheimia haemolytica, Histophilus somni, and Arcanobacterium pyogenes. For each pathogen, 12 perfect-match oligonucleotide probes, which were also designed in-house, targeted different sections of the respective 16S ribosomal genes, and were coupled with 12 corresponding mismatched probes for background. These arrays were able to produce distinct hybridization patterns for each pathogen that were easily visible without the need for …