Digital Commons Network^™

Increasing Expression Of Hepatitis B Surface Antigen In Maize Through Breeding, Erin Suzanne Miller

Master's Theses

The hepatitis B virus (HBV) is a common virus, with two billion people infected worldwide. It causes approximately 600,000 deaths each year, despite the availability of an effective vaccine since 1982. Maize as a platform for oral vaccination can supply a heat stable vaccine, which does not require syringes or trained personnel to administer. The Hepatitis B Surface antigen was transformed into maize and this seed was used to evaluate expression levels through the breeding process. The transgene was transferred into two elite maize inbreds by backcrossing. Highest expressing ears were selected each generation until approximately 99% commercial parent was …

Methane Production By A Packed-Bed Anaerobic Digester Fed Dairy Barn Flush Water, Sean Richard Thomson

Master's Theses

Packed-bed digesters are an alternative to covered lagoon digesters for methane production and anaerobic treatment of dilute wastewaters such as dairy barn flush water. The physical media of packed-beds retain biofilms, often allowing increased treatment rates. Previous studies have evaluated several types of media for digestion of dilute wastewaters, but cost and media fouling have setback commercial development. A major operational cost has been effluent recirculation pumping.

In the present effort, a novel approach to anaerobic digestion of flush dairy water was developed at pilot-scale: broken walnut shells were used as a low-cost packed-bed medium and effluent recirculation was replaced …

Studies To Characterize Heavy Metal Content And Migration From Recycled Polyethyleneterephthalate, Michael John-Ross Whitt

Master's Theses

Packaging Materials account for 31% of the world’s municipal solid waste. Agencies like the Environmental Protection Agency (EPA) and the Agency for Toxic Substances and Disease Registry (ATSDR) are pushing for the increased use of recycled thermoplastic materials. Polyethylene terephthalate (PET) is a commonly recycled thermoplastic which is used to package ready-to-eat fruits and vegetables. Most recycled polyethylene terephthalate (RPET) packaging materials contain heavy metal catalysts, the most common being antimony. The recent increased use of recycled plastic materials has been suspected as the source of increased human heavy metal exposure. In this study, cadmium, chromium, nickel, lead and antimony …

Effects Of Chelating Agents On Texture Of Lowfat Cheddar Cheese, Mariela Fernanda Poveda

Master's Theses

Effects of two types of chelating agents on proteolysis and texture properties of low fat Cheddar cheese (LFC) were analyzed and compared to full fat Cheddar (FFC) control during ripening for 120 days at 8°C. We hypothesized that chelating agents would bind calcium ions from cheese matrix to give a softer curd due to a decrease of protein-protein interactions and simultaneously increasing moisture content. Cheese milk containing (0.59% fat) was divided into three lots (A, B & C). Sodium citrate (3Na) and disodium EDTA (EDTA) were added to A & B at the rate of (0.02% and 0.2% respectively. C …

Urban Agriculture Stormwater Management In California Cities, Rachel L. Cohen

Master's Theses

Cities within California are beginning to incorporate urban agriculture into their land use designations. Prompted by residents and local organizations, cities are hoping to capture the benefits that urban agriculture provides. Research has shown that urban agriculture renews and beautifies neighborhoods, provides healthy food choices, increases public health, has the potential to help with stormwater runoff, creates jobs, and fosters community. In the last few years, several California cities have made headlines as they have adopted new zoning codes that include urban agriculture.

In reviewing these new zoning codes and exploring the topic of urban agriculture, it became evident that …

Effect Of Ph And Temperature On The Binding Interactions Of Caffeine And Chlorogenic Acid With Sodium Caseinate, Emily S. Kong

Master's Theses

Coffee is a popular and well-loved beverage consumed worldwide by millions of people every day. While most patrons of coffee do so because of its unique and satisfying taste, consumers may be unaware of the potential beneficial health effects it also imparts. The antioxidants found in green coffee beans collectively known as chlorogenic acids (CGA) and caffeine are two of the most abundant bioactive compounds present in coffee. Both these bioactive compounds have been implicated in many studies to impart a wide range of health benefits, from reducing the risk of Type 2 diabetes, to their use as aides in …

Effect Of Post Manufacture Thermal Dip Treatment On Proteolysis Of Commercial String Cheese During Storage, Melissa Karen Hsu

Master's Theses

String cheese, a Mozzarella cheese, has the unique ability to string in fibrous strands when pulled apart. Graders judge string cheese by its stringy texture; samples with copious amounts of string are awarded high ratings. But just as the texture of natural cheeses softens with time, the stringy texture of string cheese can diminish with age too.

Age related softening in cheese is due primarily to an important biochemical event known as proteolysis, which is attributed to inherent milk proteinases, residual coagulant activity, and enzymes from the lysis of starter culture microorganisms. It is hypothesized that a post manufacture heat …

Effects Of Silvicultural Management On Coast Redwood Forest Composition, Density And Structure In Santa Cruz And San Mateo Counties, Michael J. Papa

Master's Theses

The multiple-objective exploratory study investigates effects of various silvicultural management regimes commonly applied to coast redwood (Sequoia sempervirens [D. Don] Endl.) forests in Santa Cruz and San Mateo Counties, California, USA. A temporary forest inventory was installed in 24 harvest origin stands and 4 natural origin stands throughout the study area (sample area = 1189 acres). Data from the systematic sample of 233 one-quarter acre nested cluster plots (sample intensity = 4.9%) rendered overall forest descriptions in terms of species composition, density, and structure. The common forestry measures of trees per acre (TPA), basal area per acre (BA), and …

Mortality Assessment Of Redwood And Mixed Conifer Forest Types In Santa Cruz County Following Wildfire, Steve R. Auten

Master's Theses

On August 12, 2009, the Lockheed Fire ignited the west slope of the Santa Cruz Mountains burning approximately 7,819 acres. Foresters and other land managers were left with challenging decisions on how to evaluate tree mortality. Big Creek Lumber Company, California Polytechnic State University, San Luis Obispo (Cal Poly)’s Swanton Pacific Ranch (SPR), and other resource professionals familiar with this region teamed up to develop a method for evaluating damage and thereby mortality for redwood, California nutmeg, live oak, tanoak, California bay, Pacific madrone, big leaf maple, Douglas-fir, Monterey pine, and knobcone pine. Quantitative damage criteria were used to design …

Effects Of Compost, Legume Cover Cropping And Vermicompost Extract Foliar Applications On Nutrition And Yield Of Washington Navel Oranges, William Payton Carling

Master's Theses

ABSTRACT

Effects of Compost, Legume Cover Cropping and Vermicompost Extract Foliar Applications on Nutrition and Yield of Washington Navel Oranges

William Payton Carling

An experiment was conducted to test the effects of four treatments on Washington navel orange (Citrus sinensis) trees in regards to nutrient content of the leaves and fruit, soil nutrient content and properties, and fruit yield. The four treatments included: compost (C) and vermicompost extract foliar (VEF) applications, legume cover cropping (LCC) and VEF applications, VEF applications, and a control. The treatments were implemented from February 2010 to April 2011 and sampling occurred in May …

Evaluation Of Viability Of Lactobacillus Acidophilus La-5 During Simulated Digestion Process Using A Dynamic In Vitro Model, Jenifer Tharani

Master's Theses

In recent years, there has been an upsurge in medical research assessing the therapeutic benefits of probiotic bacteria and growing commercial interest in food fortification with these bacteria. Probiotic bacteria such as L. acidophilus are known to be predominant Lactobacilli species in the intestinal tract of healthy humans and suggested to provide clinical health benefits such as enhancement of immunity against intestinal infections, prevention of diarrhea and hypercholesterolaemia and improvement in lactose utilization. Many studies have demonstrated the possibility of incorporating probiotic bacteria in an ice cream matrix and shown its viability can be maintained throughout the shelf life of …

Strawberry Growth, Yield, Fruit Nutrition, And Control Of Verticillium Wilt With Pre-Plant Soil Fumigants, Ozone, And Biological Control, Justin J. Scurich

Master's Theses

Verticillium wilt is a widespread soilborne disease of strawberry historically controlled by soil fumigation with methyl bromide (MB). MB was banned by the United Nations in 1995 and will be completely phased out by 2015. Research has concentrated on alternative methods of disease control without finding a single alternative able to replace MB in widespread disease control and yield increase. For the current study, strawberries were greenhouse grown in container pots filled with soil from both infested and non-infested areas of a commercial strawberry field in Watsonville, CA. Treatments included pre-plant soil fumigation with commercially available formulations of methyl bromide, …

Investigation Of Bacillus Subtilis As A Biopesticide Against Botrytis Cinerea, Kenneth K. Ng

Master's Theses

The objective of this thesis was to investigate BiOWiSH^TM-Aqua, a commercial dry solid formulation containing a consortium of bacteria and yeast, as a biopesticide for treatment of Botrytis cinerea, a gray mold that affects strawberries. BiOWiSH^TM-Aqua was compared with another commercial product specifically used as a fungicide and bacteriocide, Serenade® Garden Disease Control Spray (concentrated Bacillus subtilis strain QST 713). Both laboratory tests as well as in vivo lab tests were conducted. BiOWiSH^TM-Aqua results varied widely from plate to plate, regardless of experimental conditions. In some of these plates, inhibition zones were observed …

The Influence Of Chilling And Heat Accumulation On Bloom Timing, Bloom Length And Crop Yield In Almonds (Prunus Dulcis (Mill.)), Melanie M. Covert

Master's Theses

Almonds are one of the first commercial nut trees to bloom in early spring and thus are susceptible to temperature patterns prior to and during bloom which affect bloom timing, bloom length, pollination and nut set. Data used in this project include yearly dates of 90% bloom from 1996-2006, bloom length in days and final crop yields in pounds per tree for Nonpareil and Mission varieties. Data were collected from the University of California Cooperative Extension reports on the 1993-2006 Regional Almond Variety Trials in Butte, San Joaquin and Kern Counties. Temperature pattern models in the form of Chill Hours …

Influence Of Vineyard Floor Management Practices On Soil Aggregate Stability, Total Soil Carbon And Grapevine Yield, Kimberly Ellen Adams

Master's Theses

Soil aggregates provide pore spaces of various sizes supplying water, gases and nutrients to plant roots and microorganisms, and facilitate moisture retention and availability. Soil aggregate stability is indicative of soil biological and structural health, and is increased by soil carbon derived from plant roots and the soil microbial biomass. Aggregate stability and soil carbon can be enhanced by increasing organic matter through compost additions or by planting cover crops. Additionally, aggregate stability is enhanced by arbuscular mycorrhizal fungi (AMF) that form a symbiotic association with plant roots, and consolidate soil particles into aggregates through hyphal networks and through the …

Evaluation Of Vacuum Packaging On The Physical Properties, Solubility, And Storage Space Of Dairy Powders, Hadi Eshpari

Master's Theses

As many of the dairy powders manufactured have to travel long distances to reach their customers, both domestically and internationally, there is considerable interest among dairy powder manufacturers to maintain the quality of their products for relatively long storage periods. Dairy powders can have a long shelf life if packaged and stored properly. Vacuum packaging can be an attractive packaging strategy to maintain the quality of dairy powders and provide added value by improving the efficiency of using the storage space; because of the inherent compactness of these products. Vacuum packaged dry dairy ingredients may also have added ease of …

Predicting Soil Erosion In The Santa Rosa Creek Watershed Using Rusle2 And Geographic Information Systems, Stacey Carol Smith

Master's Theses

The Santa Rosa Creek watershed is one of the most pristine watersheds on California’s Central Coast. Preserving this watershed is of great interest because it provides rich soils for agriculture, vast rangelands for cattle, and flowing streams for federally threatened species such as steelhead trout. Soil erosion could impact these resources. Using prediction tools, it is possible to study the erosion that could be occurring in a watershed and identify locations which could contribute the highest amounts of sediment. The objectives of this study were to use RUSLE2 and Geographic Information Systems (GIS) to predict soil erosion rates for each …

Isolation Of Antifungal Lactic Acid Bacteria From Food Sources And Their Use To Inhibit Mold Growth In Cheese, Dan Zhao

Master's Theses

A large amount of cheese is lost every year due to mold contamination. Biopreservation, which is the use of biological entities (microbes) and their metabolites to suppress microbial spoilage instead of chemical preservatives has lately gained increasing interest. Lactic acid bacteria (LAB) have the potential for use in biopreservation, because they are safe to consume and naturally exist in many foods. In this study, fifteen strains of lactobacilli isolated from dairy products, vegetables, and fermented pickles were tested by agar overlay assay for their anti-mold activity. Six strains grown on MRS agar showed strong inhibitory activity against a target mold …

Evaluation Of Drip Applications And Foliar Sprays Of The Biocontrol Product Actinovate On Powdery Mildew And Other Fungal Diseases Of Tomato, Therese Angelica Quintana-Jones

Master's Theses

The effectiveness of the biocontrol product Actinovate^® at enhancing tomato plant growth and yield, and reducing the presence of fungal pathogens was studied in greenhouse and field conditions. In the greenhouse, no differences were found among seed germination or plant survival rates, seedling heights, dry root weights, and dry shoot weights of tomato seedlings grown from seeds drenched with Actinovate^® or Rootshield^®. The effects of one initial Actinovate^® seed drench at sowing, repeated applications through the drip irrigation throughout the season, or repeated applications through the drip irrigation plus foliar applications throughout the season at …

The Effect Of Processing Parameters And Methylcellulose On Texture And Consumer Acceptability Of A Non-Melting Dairy Protein Gel, Joshua Goldman

Master's Theses

The overall goal was to understand the capabilities of a dairy based meat alternative. This was done in three phases: 1) the production of a dairy protein gel as a base for a dairy based meat alternative, 2) texturization of the dairy protein gel base to produce a nugget like texture, and 3) a consumer test to determine the acceptability of the dairy based nugget compared to meat and meat alternatives on the market.

For phase I, a dairy protein gel base was tested to understand the textural attributes. Milk to whey percentages and level of acidification were examined to …

Combining Conventional Tests And Terminal Restriction Fragment Analysis To Evaluate Microbial Quality Of Raw Milk, Haibin Guo

Master's Theses

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Abstract

Combining Conventional Tests and Terminal Restriction Fragment Analysis to Evaluate Microbial Quality of Raw Milk

Haibin Guo

The dairy industry is an important part in the domestic economy in the U.S. and the quality of dairy products hinges on raw milk quality. Microorganisms play a critical role in raw milk quality and they are currently tested and monitored by conventional microbiological tests. …

Effects Of Pruning Level And Canopy Management Practices On Berry Maturation Rate And Harvest Parameters Of Syrah Wine Grapes, Jeffery S. Landolt

Master's Theses

Syrah is an important wine grape in California but is potentially difficult to manage in the vineyard due to its excessive vigor. Vigorous grapevines require more labor for canopy management and tend to create excess shade, decreasing fruit quality. Winter pruning level, shoot thinning and leaf removal influence the overall density of the canopy and the subsequent degree of shade in the fruit zone. An experiment was conducted to assess the effects of two pruning levels with three degrees of labor-intensive canopy management techniques on berry maturation rate and harvest berry parameters for two growing seasons. In 2008, repeated measures …