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2017

College of Agriculture and Life Sciences Faculty Publications

High-variation regions

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The Complete Chloroplast Genome Of Primulina And Two Novel Strategies For Development Of High Polymorphic Loci For Population Genetic And Phylogenetic Studies, Chao Feng, Meizhen Xu, Chen Feng, Eric J.B. Von Wettberg, Ming Kang Nov 2017

The Complete Chloroplast Genome Of Primulina And Two Novel Strategies For Development Of High Polymorphic Loci For Population Genetic And Phylogenetic Studies, Chao Feng, Meizhen Xu, Chen Feng, Eric J.B. Von Wettberg, Ming Kang

College of Agriculture and Life Sciences Faculty Publications

Background: Primulina Hance is an emerging model for studying evolutionary divergence, adaptation and speciation of the karst flora. However, phylogenetic relationships within the genus have not been resolved due to low variation detected in the cpDNA regions. Chloroplast genomes can provide important information for phylogenetic and population genetic studies. Recent advances in next-generation sequencing (NGS) techniques greatly facilitate sequencing whole chloroplast genomes for multiple individuals. Consequently, novel strategies for development of highly polymorphic loci for population genetic and phylogenetic studies based on NGS data are needed. Methods: For development of high polymorphic loci for population genetic and phylogenetic studies, two …