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Microbial Synthesis And Characterization Of Silver Nanoparticles Using Alternaria Alternata, Sarafadeen O. Kareem, Oluwagbenga T. Familola, Adejare R. Oloyede, Enock O. Dare

Applied Environmental Research

In this study, extracellular biosynthesis of silver nanoparticles (AgNPs) was carried out using Alternaria alternata. AgNPs were synthesized using vegetative and cell-free filtrate methods. Silver nitrate (AgNO3) solution was reduced with fungal mycelial mass and cell-free filtrate at a ratio of 1:4 (v/v). Further, the effects of biosynthesis parameters (reaction time, reaction temperature, light and dark incubation, and static and agitated conditions) were determined. The structural integrity of the synthesized AgNPs was investigated through UV-visible spectrophotometry, X-ray diffraction (XRD) analysis, and transmission electron microscopy (TEM). Parameter optimization revealed that cell-free filtrate AgNP synthesis at 40 °C with constant agitation and …