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Full-Text Articles in Social and Behavioral Sciences
Single Nucleotide Primer Extension (Snupe) Analysis Of The G6pd Gene In Somatic Cells And Oocytes Of A Kangaroo (Macropus Robustus), Debbie Watson, Anita S. Jacombs, David A. Loebel, Edward S. Robinson, Peter G. Johnston
Single Nucleotide Primer Extension (Snupe) Analysis Of The G6pd Gene In Somatic Cells And Oocytes Of A Kangaroo (Macropus Robustus), Debbie Watson, Anita S. Jacombs, David A. Loebel, Edward S. Robinson, Peter G. Johnston
Faculty of Science, Medicine and Health - Papers: part A
cDNA sequence analysis of the X-linked glucose-6-phosphate dehydrogenase (G6PD) gene has shown a base difference between two subspecies of the kangaroo, Macropus robustus robustus (wallaroo) and M. r. erubescens (euro). A thymine residue in the wallaroo at position 358 in exon 5 has been replaced by a cytosine residue in the euro, which accounts for the previously reported electrophoretic difference between the two subspecies. This base difference allowed use of the Single Nucleotide Primer Extension (SNuPE) technique to study allele-specific expression of G6PD at the transcriptional level. We began by examining G6PD expression in somatic cells and observed complete paternal …
Role Of Phosphoglucomutase Of Bordetella Bronchiseptica In Lipopolysaccharide Biosynthesis And Virulence, Nicholas P. West, Heidrun Jungnitz, John Fitter, Jason D. Mcarthur, Carlos Guzman, Mark J. Walker
Role Of Phosphoglucomutase Of Bordetella Bronchiseptica In Lipopolysaccharide Biosynthesis And Virulence, Nicholas P. West, Heidrun Jungnitz, John Fitter, Jason D. Mcarthur, Carlos Guzman, Mark J. Walker
Faculty of Science, Medicine and Health - Papers: part A
The phosphoglucomutase (PGM)-encoding gene of Bordetella bronchiseptica is required for lipopolysaccharide (LPS) biosynthesis. An insertion mutant of the wild-type B. bronchiseptica strain BB7865 which disrupted LPS biosynthesis was created and characterized (BB7865pgm). Genetic analysis of the mutated gene showed it shares high identity with PGM genes of various bacterial species and forms part of an operon which also encompasses the gene encoding phosphoglucose isomerase. Functional assays for PGM revealed that enzyme activity is expressed in bothbvg-positive and bvg-negative strains ofB. bronchiseptica and is substantially reduced in BB7865pgm. Complementation of the mutated PGM …
Clusterin Protein Diversity In The Primate Eye, Paul Wong, Bruce A. Pfeffer, Steven L. Bernstein, Michelle L. Chambers, Gerald J. Chader, Zahra F. Zakeri, Yan-Q Wu, Mark Wilson, S Patricia Becerra
Clusterin Protein Diversity In The Primate Eye, Paul Wong, Bruce A. Pfeffer, Steven L. Bernstein, Michelle L. Chambers, Gerald J. Chader, Zahra F. Zakeri, Yan-Q Wu, Mark Wilson, S Patricia Becerra
Faculty of Science, Medicine and Health - Papers: part A
Purpose: The clusterin gene encodes a multi-functional protein that has been identified in different tissues, including a number of different eye tissues, primarily in the mouse and to a much lesser extent in humans. Clusterin has been implicated in a number of cellular processes such as lipid transport, membrane integrity, apoptosis, and neurodegeneration, all of which could be important to the biology of the eye. In the current communication, we provide data that confirms the expression of clusterin in a number of different human eye tissues and establishes the expression profile of this gene in monkey derived eye tissues. The …