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Full-Text Articles in Social and Behavioral Sciences
Biosorption Of Lac Dye By The Red Marine Alga Gracilaria Tenuistipitata: Biosorption Kinetics, Isotherms, And Thermodynamic Parameters, Montra Chairat, John B. Bremner
Biosorption Of Lac Dye By The Red Marine Alga Gracilaria Tenuistipitata: Biosorption Kinetics, Isotherms, And Thermodynamic Parameters, Montra Chairat, John B. Bremner
Faculty of Science, Medicine and Health - Papers: part A
The hypothesis that the dried, ground biomass of the red marine alga Gracilaria tenuistipitata could be used for the efficient removal of lac dye from aqueous solution was assessed in this work. The effects of parameters such as initial pH, biosorbent dosage, contact time, initial dye concentration, and temperature on the biosorption capacity of the dye were investigated. Equilibrium data were analysed using Langmuir, Freundlich, and Temkin isotherm models, and the Freundlich model provided the highest coefficient of determination values. Biosorption kinetic data were successfully described with a pseudo-second-order model at initial dye concentrations of 50, 80, 100, and 120 …
Single-Molecule Specific Mislocalization Of Red Fluorescent Proteins In Live Escherichia Coli, Harshad Ghodke, Victor E. A Caldas, Christiaan M. Punter, Antoine M. Van Oijen, Andrew Robinson
Single-Molecule Specific Mislocalization Of Red Fluorescent Proteins In Live Escherichia Coli, Harshad Ghodke, Victor E. A Caldas, Christiaan M. Punter, Antoine M. Van Oijen, Andrew Robinson
Faculty of Science, Medicine and Health - Papers: part A
Tagging of individual proteins with genetically encoded fluorescent proteins (FPs) has been used extensively to study localization and interactions in live cells. Recent developments in single-molecule localization microscopy have enabled the dynamic visualization of individual tagged proteins inside living cells. However, tagging proteins with FPs is not without problems: formation of insoluble aggregates and inhibition of native functions of the protein are well-known issues. Previously reported artifacts manifest themselves at all expression levels of the FP-tagged proteins, making the design of control experiments relatively straightforward. Here, we describe a previously uncharacterized mislocalization artifact of Entacmaea quadricolor red fluorescent protein variants …