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Differentiation Of Bone Marrow Stem Cells On Inkjet Printed Silk Lines, Skander Limem, Paul Calvert, Hyeon Joo Kim, David L. Kaplan

Faculty of Science, Medicine and Health - Papers: part A

Water based silk solutions were successfully inkjet printed for the first time into patterns of parallel lines onto vinyl plastic substrates. Human bone marrow stromal cells (hMSCs) were seeded on the silk printed patterns and cultured in the presence of 100 ng/ml of bone morphogenic protein (BMP-2). After one week of culture cell growth and attachment showed site specificity on the silk printed lines. Both alkaline phosphatase activity and cell morphology indicated hBMSCs differentiation into osteogenic cells along the silk printed lines. After 4 week of culture, the cellular bridging of adjacent silk printed lines took place for all interline …