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Full-Text Articles in Social and Behavioral Sciences
Bovine Non-O157 Shiga Toxin 2-Containing Escherichia Coli Isolates Commonly Possess Stx2-Edl933 And/Or Stx2vhb Subtypes, Kim Brett, Michael A Hornitzky, Karl A Bettelheim, Mark J. Walker, Steven Djordjevic
Bovine Non-O157 Shiga Toxin 2-Containing Escherichia Coli Isolates Commonly Possess Stx2-Edl933 And/Or Stx2vhb Subtypes, Kim Brett, Michael A Hornitzky, Karl A Bettelheim, Mark J. Walker, Steven Djordjevic
Faculty of Science, Medicine and Health - Papers: part A
stx2 genes from 138 Shiga toxin-producing Escherichia coli (STEC) isolates, of which 127 were of bovine origin (58 serotypes) and 11 of human origin (one serotype; O113:H21), were subtyped. The bovine STEC isolates from Australian cattle carried ehxA and/or eaeA and predominantly possessed stx2-EDL933 (103 of 127; 81.1%) either in combination with stx2vhb (32 of 127; 25.2%) or on its own (52 of 127; 40.4%). Of 22 (90.9%) bovine isolates of serotype O113:H21, a serotype increasingly recovered from patients with hemolytic uremic syndrome (HUS) or hemorrhagic colitis, 20 contained both stx2-EDL933 and stx2vhb; 2 …
Distribution Of Intimin Subtypes Among Escherichia Coli Isolates From Ruminant And Human Sources, Vidiya Ramachandran, Kim Brett, Michael A Hornitzky, Mark Dowton, Karl A Bettelheim, Mark J. Walker, Steven P. Djordjevic
Distribution Of Intimin Subtypes Among Escherichia Coli Isolates From Ruminant And Human Sources, Vidiya Ramachandran, Kim Brett, Michael A Hornitzky, Mark Dowton, Karl A Bettelheim, Mark J. Walker, Steven P. Djordjevic
Faculty of Science, Medicine and Health - Papers: part A
The intimin gene eae, located within the locus of enterocyte effacement pathogenicity island, distinguishes enteropathogenic Escherichia coli (EPEC) and some Shiga toxin-producing E. coli (STEC) strains from all other pathotypes of diarrheagenic E. coli. EPEC is a leading cause of infantile diarrhea in developing countries, and intimin-positive STEC isolates are typically associated with life-threatening diseases such as hemolytic-uremic syndrome and hemorrhagic colitis. Here we describe the development of a PCR-restriction fragment length polymorphism (RFLP) assay that reliably differentiates all 11 known intimin types (α1, α2, β, γ, κ, ɛ, η, ι, λ, θ, and ζ) and three new …