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Full-Text Articles in Social and Behavioral Sciences
The Structure And Properties Of Methylenetetrahydrofolate Reductase From Escherichia Coli Suggest How Folate Ameliorates Human Hyperhomocysteinemia, Brian D. Guenther, Christal A. Sheppard, Pamela Tran, Rima Rozen, Rowena G. Matthews, Martha L. Ludwig
The Structure And Properties Of Methylenetetrahydrofolate Reductase From Escherichia Coli Suggest How Folate Ameliorates Human Hyperhomocysteinemia, Brian D. Guenther, Christal A. Sheppard, Pamela Tran, Rima Rozen, Rowena G. Matthews, Martha L. Ludwig
Nebraska College of Law: Faculty Publications
Elevated plasma homocysteine levels are associated with increased risk for cardiovascular disease and neural tube defects in humans. Folate treatment decreases homocysteine levels and dramatically reduces the incidence of neural tube defects. The flavoprotein methylenetetrahydrofolate reductase (MTHFR) is a likely target for these actions of folate. The most common genetic cause of mildly elevated plasma homocysteine in humans is the MTHFR polymorphism A222V (base change C677→T). The X-ray analysis of E. coli MTHFR, reported here, provides a model for the catalytic domain that is shared by all MTHFRs. This domain is a β8α8 barrel that binds FAD …
Purification And Properties Of Nadh-Dependent 5,10-Methylenetetrahydrofolate Reductase (Metf) From Escherichia Coli, Christal A. Sheppard, Elizabeth E. Trimmer, Rowena G. Matthews
Purification And Properties Of Nadh-Dependent 5,10-Methylenetetrahydrofolate Reductase (Metf) From Escherichia Coli, Christal A. Sheppard, Elizabeth E. Trimmer, Rowena G. Matthews
Nebraska College of Law: Faculty Publications
A K-12 strain of Escherichia coli that overproduces methylenetetrahydrofolate reductase (MetF) has been constructed, and the enzyme has been purified to apparent homogeneity. A plasmid specifying MetF with six histidine residues added to the C terminus has been used to purify histidine-tagged MetF to homogeneity in a single step by affinity chromatography on nickel-agarose, yielding a preparation with specific activity comparable to that of the unmodified enzyme. The native protein comprises four identical 33-kDa subunits, each of which contains a molecule of noncovalently bound flavin adenine dinucleotide (FAD). No additional cofactors or metals have been detected. The purified enzyme catalyzes …
Random Questions About Law School And The Law: The World's First Socratic Law Review Article, C. Steven Bradford
Random Questions About Law School And The Law: The World's First Socratic Law Review Article, C. Steven Bradford
Nebraska College of Law: Faculty Publications
Many law professors utilize the Socratic method in their teaching, presumably because wisdom is to be found in the questions them selves. Yet, no law professors use the Socratic method in their writing. Why? I believe it is a wise marketing decision by law professors. Anyone off the street can read a law review article, and receive its wisdom for free. If law professors disclosed their full Socratic wisdom in law reviews, no one would come to law school, and law professors would be unemployed. By withholding their Socratic questions from law reviews, law professors have forced students to pay …
Expanding The Investment Company Act: The Sec's Manipulation Of The Definition Of Security, C. Steven Bradford
Expanding The Investment Company Act: The Sec's Manipulation Of The Definition Of Security, C. Steven Bradford
Nebraska College of Law: Faculty Publications
The Securities and Exchange Commission (SEC) is sometimes accused of using the administrative process "to graft new, substantive standards and obligations onto existing statutes or SEC rules." Through the use of the no-action letter process, the SEC staff sometimes creates substantive securities law which is inconsistent with the relevant federal securities statutes and case law. Many of these staff positions "go far beyond reasonable and fair explanations of existing statutes or SEC rules." However, in substantive areas lacking definitive case law or rules, these no-action letters assume an extraordinary importance to securities lawyers and regulated entities. For all practical purposes, …