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Full-Text Articles in Social and Behavioral Sciences
Comparison Of Two Methods For Detection Of Cryptosporidium & Giardia In Water, Eva C. Nieminski, Frank W. Schaefer Iii, Jerry E. Ongerth
Comparison Of Two Methods For Detection Of Cryptosporidium & Giardia In Water, Eva C. Nieminski, Frank W. Schaefer Iii, Jerry E. Ongerth
Faculty of Engineering and Information Sciences - Papers: Part A
The steps of two immunofluorescent-antibody-based detection methods were evaluated for their efficiencies in detecting Giardia cysts and Cryptosporidium oocysts. The two methods evaluated were the American Society for Testing and Materials proposed test method for Giardia cysts and Cryptosporidium oocysts in low-turbidity water and a procedure employing sampling by membrane filtration, Percoll-Percoll step gradient, and immunofluorescent staining. The membrane filter sampling method was characterized by higher recovery rates in all three types of waters tested: raw surface water, partially treated water from a flocculation basin, and filtered water. Cyst and oocyst recovery efficiencies decreased with increasing water turbidity regardless of …
Identification Of Giardia Lamblia-Specific Antigens In Infected Human And Gerbil Feces By Western Immunoblotting, Henry H. Stibbs, Mansour Samadpour, Jerry E. Ongerth
Identification Of Giardia Lamblia-Specific Antigens In Infected Human And Gerbil Feces By Western Immunoblotting, Henry H. Stibbs, Mansour Samadpour, Jerry E. Ongerth
Faculty of Engineering and Information Sciences - Papers: Part A
Western immunoblot analysis of aqueous extracts of feces obtained from five giardiasis patients and from experimentally infected gerbils (Meriones unguiculatus) with rabbit antiserum to Giardia lamblia cysts has revealed antigens of three molecular weight groups. A stepladderlike, evenly-spaced set of strongly reactive antigens (darkest at a molecular weight [m.w.] of 55,000 to 70,000) appeared in the gerbil feces from day 4 (first experiment) or day 2 (second experiment) and lasted to about day 7 but disappeared completely by day 8 and did not reappear later. These antigenic bands were seen in gerbils infected with two isolates of G. lamblia. These …