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Applied Environmental Research
Decolorization kinetics; Melanoidin; Laccase; Degradation; White rot fungi; Megaspororia sp.
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Full-Text Articles in Social and Behavioral Sciences
Potential Degradation And Kinetics Of Melanoidin By Using Laccase From White Rot Fungus, Wittawat Toomsan, Pinthita Mungkarndee, Sophon Boonlue, Nguyen Thanh Giao, Sumana Siripattanakul-Ratpukdi
Potential Degradation And Kinetics Of Melanoidin By Using Laccase From White Rot Fungus, Wittawat Toomsan, Pinthita Mungkarndee, Sophon Boonlue, Nguyen Thanh Giao, Sumana Siripattanakul-Ratpukdi
Applied Environmental Research
This study was attempted to use laccase extracted from white rot fungus to removemelanoidin in the ethanol production wastewater. The isolated fungus producing the highest laccase was identified as Megaspororiasp. The highest degradation efficiencies of the purified and crude laccases were 48.00% and 44.60%, respectively. Both degradation kinetics well fit Michaelis-Menten model. The Michaelis constant (Km) and maximum rate of reaction (Vmax) were 0.82% melanoidin and 0.0045% melanoidin h-1 for the degradation by the purified laccase and 0.71% melanoidin and 0.0037% melanoidin h-1 for the degradation by the crude laccase. Turnover number (Kcat) of purified and crude laccases were 0.00023 …