Social and Behavioral Sciences Commons^™

Insights From Characterizing Extinct Human Gut Microbiomes, Raul Y. Tito, Dan Knights, Jessica Metcalf, Alexandra J. Obregon-Tito, Lauren M. Cleeland, Fares Najar, Bruce Roe, Karl Reinhard, Kristin D. Sobolik, Samuel L. Belknap, Morris Foster, Paul Spicer, Rob Knight, Cecil M. Lewis Jr.

Sociology & Anthropology Faculty Publications

In an effort to better understand the ancestral state of the human distal gut microbiome, we examine feces retrieved from archaeological contexts (coprolites). To accomplish this, we pyrosequenced the 16S rDNA V3 region from duplicate coprolite samples recovered from three archaeological sites, each representing a different depositional environment: Hinds Cave (~8000 years B.P.) in the southern United States, Caserones (1600 years B.P.) in northern Chile, and Rio Zape in northern Mexico (1400 years B.P.). Clustering algorithms grouped samples from the same site. Phyletic representation was more similar within sites than between them. A Bayesian approach to source-tracking was used to …