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Ozone Induced Dissociation: Elucidation Of Double Bond Position Within Mass-Selected Lipid Ions, Michael C. Thomas, Todd W. Mitchell, David G. Harman, Jane M. Deeley, Jessica R. Nealon, Stephen J. Blanksby
Ozone Induced Dissociation: Elucidation Of Double Bond Position Within Mass-Selected Lipid Ions, Michael C. Thomas, Todd W. Mitchell, David G. Harman, Jane M. Deeley, Jessica R. Nealon, Stephen J. Blanksby
Stephen Blanksby
Ions formed from lipids during electrospray ionization of crude lipid extracts have been mass-selected within a quadrupole linear ion trap mass spectrometer and allowed to react with ozone vapor. Gas-phase ion−molecule reactions between unsaturated lipid ions and ozone are found to yield two primary product ions for each carbon−carbon double bond within the molecule. The mass-to-charge ratios of these chemically induced fragments are diagnostic of the position of unsaturation within the precursor ion. This novel analytical technique, dubbed ozone-induced dissociation (OzID), can be applied both in series and in parallel with conventional collision-induced dissociation (CID) to provide near-complete structural assignment …
Online Ozonolysis Methods For The Determination Of Double Bond Position In Unsaturated Lipids, Michael Thomas, Todd W. Mitchell, Stephen J. Blanksby
Online Ozonolysis Methods For The Determination Of Double Bond Position In Unsaturated Lipids, Michael Thomas, Todd W. Mitchell, Stephen J. Blanksby
Stephen Blanksby
Modern lipidomics relies heavily on mass spectrometry for the structural characterization and quantification of lipids of biological origins. Structural information is gained by tandem mass spectrometry (MS/MS) whereby lipid ions are fragmented to elucidate lipid class, fatty acid chain length, and degree of unsaturation. Unfortunately, however, in most cases double bond position cannot be assigned based on MS/MS data alone and thus significant structural diversity is hidden from such analyses. For this reason, we have developed two online methods for determining double bond position within unsaturated lipids; ozone electrospray ionization mass spectrometry (OzESI-MS) and ozone-induced dissociation (OzID). Both techniques utilize …