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Full-Text Articles in Social and Behavioral Sciences
Decreased Heat Stability And Increased Chaperone Requirement Of Modified Human Βb1-Crystallins, Kirsten J Lampi, Yung H. Kim, Hans Peter Bachinger, Bruce A. Boswell, Robyn A. Lindner, John A. Carver, Thomas R Shearer, Larry L. David, Deborah M. Kapfer
Decreased Heat Stability And Increased Chaperone Requirement Of Modified Human Βb1-Crystallins, Kirsten J Lampi, Yung H. Kim, Hans Peter Bachinger, Bruce A. Boswell, Robyn A. Lindner, John A. Carver, Thomas R Shearer, Larry L. David, Deborah M. Kapfer
Faculty of Science, Medicine and Health - Papers: part A
Purpose: To determine how deamidation and partial loss of the N- and C-terminal extensions alter the heat stability of βB1-crystallin.
Methods: Human lens βB1, a deamidated βB1, Q204E, and αA-crystallins were expressed. Truncated βB1 was generated by proteolytic removal of part of its terminal extensions. The aggregation and precipitation of these proteins due to heating was monitored by circular dichroism and light scattering. The effect of heat on the stability of both monomers and oligomers was investigated. The flexibility of the extensions in wild type and deamidated βB1 was assessed by 1H NMR spectroscopy.
Results: With heat, deamidated βB1 …