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Performance Of A Dna And Protein Co-Extraction With Trypsin In Comparison To Two Proteinase K Based Dna Extraction Methods, Dinura A. Gunatilake
Performance Of A Dna And Protein Co-Extraction With Trypsin In Comparison To Two Proteinase K Based Dna Extraction Methods, Dinura A. Gunatilake
Student Theses
Effective DNA extraction methods are important for forensic applications. The main goal of this experiment was to determine if a newly developed trypsin based protein/DNA co-extraction method applied to contact traces would yield comparable results to a commercial Proteinase K method (QIAamp DNA Investigator Kit) and a Chelex extraction method with Tween 20 used in forensic laboratories. This was tested on 20 sets of sebaceous fingerprints on glass slides.
The results of this study demonstrate the trypsin co-extraction method yielded the highest amount of DNA. In the first comparison, the mean total DNA yields for the trypsin-co extraction method and …