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Social and Behavioral Sciences Commons™
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Full-Text Articles in Social and Behavioral Sciences
Reproducibility Of Individual Dna Deposits Detected Through Cellular Fluorescence, Natalee Small-Davidson
Reproducibility Of Individual Dna Deposits Detected Through Cellular Fluorescence, Natalee Small-Davidson
Student Theses
Contact traces are an important part of DNA casework, but the probative value of any identified associations depends on the possibility of passive transfer. There is known individual variation in DNA left behind during contact, this DNA shedding propensity has an effect on whose DNA is detected. This study evaluated this variability using a cell staining approach. Volunteers were asked to deposit a fingerprint on a clean glass slide, then wash their hands and deposit a second fingerprint after a 30-minute wait without touching anything. Three sets of samples were collected over three consecutive weeks. Fingerprints were stained with a …
Dna Extraction And Genotyping From Burned Skeletal Remains, Kayla M. Rooney
Dna Extraction And Genotyping From Burned Skeletal Remains, Kayla M. Rooney
Student Theses
In this research, bovine leg bones were burned, both with and without meat intact, in order to determine if it was possible to extract and amplify DNA to obtain profiles. The meat was burned over an open flame and the DNA was extracted using the Qiagen DNeasy Blood & Tissue Mini Kit, quantified with the NanoDrop, and amplified using the Bovine Genotypes Panel 3.1 Kit, and separated using the 3500 Genetic Analyzer. Profiles were analyzed using the Thermo Fisher Cloud Microsatellite Analysis software. The samples burned with the meat intact produced lower quantities of DNA than the bones burned with …
Correlation Between Tooth Root Translucency, Crystallinity, And Dna Preservation In Teeth For Unidentified Human Remains (Uhr) Investigations, Paul Yount
Master's Theses
Endogenous genetic material protected by the rigid, mineralized hard tissues of bones and teeth is often targeted for forensic DNA testing in missing persons and unidentified human remains (UHR) investigations. Although standard approaches have narrowed sample selection to a few optimal skeletal elements (i.e., weight-bearing long bones, teeth), subsampling and decisions beyond this can be challenging due to the non-uniform, heterogeneous pattern of postmortem diagenesis. One particular component of skeletal microstructure — the inorganic mineral matrix [hydroxyapatite (HAp), Ca10(PO4)6(OH)2] — is purported to play a major role in DNA preservation. The potential correlation between molecular changes to skeletal microstructure and …