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Full-Text Articles in Social and Behavioral Sciences
Evaluation Of A Novel Dna Collection Matrix Designed To Improve Recovery Of Touch Dna From Non-Porous Surfaces, Eric Ramirez
Evaluation Of A Novel Dna Collection Matrix Designed To Improve Recovery Of Touch Dna From Non-Porous Surfaces, Eric Ramirez
Student Theses
The collection of touch DNA from evidence has been a challenge in the field of Forensic Biology for decades. There are numerous collection protocols, substrates, and solutions that have been used to varying levels of success. Currently, no single method or substrate stands out as the single most effective collection protocol. The Gene Link Omni Matrix™ K105 solution is a novel collection method that can be sprayed onto the surface of non-porous evidence. The matrix dries into a film, capturing cells and cell-free DNA which can be scraped off and collected into a tube. During extraction, the matrix dissolves into …
An Exploration Of Protein And Dna Components In Fingerprint Residue, Ashley Borrego
An Exploration Of Protein And Dna Components In Fingerprint Residue, Ashley Borrego
Student Theses
The main focus of this project was to investigate the protein and DNA components in both sebaceous and eccrine fingerprints. This study investigated the relative content of DNA and proteins in eccrine fingerprints to sebaceous fingerprints. All volunteers were instructed to wash and dry their hands prior to depositing parallel thumbprints. Twenty volunteers were instructed to touch their face to produce sebaceous prints, and 5 volunteers were instructed to wear gloves over a heat source to produce sweaty or eccrine prints. Microscopy was used to score the cellular debris of the right fingerprint on a scale of 1-4 based on …
Optimizing A Method For Simultaneous Recovery Of Proteins And Dna From Fingerprints, Steven Kranes
Optimizing A Method For Simultaneous Recovery Of Proteins And Dna From Fingerprints, Steven Kranes
Student Theses
DNA testing on touched objects is a valuable tool in forensic investigations, but DNA is usually present in low amounts, causing poor STR typing results. For touch DNA evidence, there is a clear need for additional individualization, especially for highly probative samples. This could be achieved by testing genetically variable proteins. The goal of this project was to develop a DNA/protein co-extraction method to facilitate DNA and protein testing on the same evidence item. Existing DNA extraction methods were carefully adjusted to allow for downstream mass spectrometry analysis. Initial experiments on saliva and fingerprints placed on glass suggested that trypsin …