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Full-Text Articles in Social and Behavioral Sciences
Extraction Of Challenging Forensic Samples Using The Microgem Dna Extraction Kit, Falyn R. Vega
Extraction Of Challenging Forensic Samples Using The Microgem Dna Extraction Kit, Falyn R. Vega
Student Theses
In forensic science, DNA extraction can be a tedious and resource-intensive process. Extraction with Proteinase K is an industry standard but has its drawbacks, such as requiring multiple ionic detergents and washing steps. MicroGEM has developed a new enzyme called forensicGEM that is temperature-dependent and compatible with mesophilic enzymes, offering complete DNA extraction in about 20 minutes in a single tube, limiting contamination, loss of sample, and working time. ForensicGEM can extract DNA from highly degraded samples, potentially leading to more complete STR profiles. Highly degraded tissue and bone samples were collected and extracted with the forensicGEM …
Developing A Maceration Method For Isolation Of Dna From Cancellous Bones, Bryan Bernal
Developing A Maceration Method For Isolation Of Dna From Cancellous Bones, Bryan Bernal
Student Theses
Prior to isolating DNA, it is necessary to remove any biological materials that may be present on commingled remains. Manual maceration of the outer surface is time-consuming. This study will address this issue by developing a simple method for processing bone samples prior to DNA isolation. A liquid-based technique was applied to macerate bone fragments by incubating bone fragments in an enzyme solution, removing potential contaminants, followed by a bone tissue disruption. Swine (Sus scrofa) bones were used to simulate human bones. Microscopic analysis suggested this method was effective for surface material removal. The methods effect on the …
Semi-Quantitative Detection Of Signature Peptides In Body Fluids By Liquid Chromatography Tandem Mass Spectrometry (Lc-Ms/Ms), Tebah N. Browne
Semi-Quantitative Detection Of Signature Peptides In Body Fluids By Liquid Chromatography Tandem Mass Spectrometry (Lc-Ms/Ms), Tebah N. Browne
Student Theses
This study covers a modified semi-quantitative approach for liquid chromatography mass spectrometry (LC-MS/MS) signature peptide detection for body fluid identification. Peptide concentrations were measured based on synthetic peptide standards. Samples were processed with varying trypsin digestion and purification protocols, including a three-hour trypsin digestion and Microcon membrane filtration. The Microcon filtration method generates polymerase chain reaction (PCR) compatible DNA and peptide fractions that can be analyzed without any further purification. Preliminary validation tests covered stains on different substrates, semen/ saliva mixtures, minimum sample volume, and repeatability. All signature peptides in the multiplex were present at different concentrations and varied amongst …