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Social and Behavioral Sciences Commons™
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Full-Text Articles in Social and Behavioral Sciences
Effects Of Microwave Radiation On The Digestion Of Proteins Involved In Body Fluid Identification, Aaron J. Fox
Effects Of Microwave Radiation On The Digestion Of Proteins Involved In Body Fluid Identification, Aaron J. Fox
Student Theses
Body fluid identification plays an important role in understanding how the events during a crime may have taken place. The presence of a body fluid may help identify an individual who committed a crime, while the type of body fluid present may help investigators determine how a crime occurred. Current body fluid identification techniques are not always conclusive and may only suggest the presence of a body fluid type. A protocol developed by the New York City Office of the Chief Medical Examiner (OCME) established a method that confirms body fluid type through mass spectrometry. To identify marker proteins in …
Semi-Quantitative Detection Of Signature Peptides In Body Fluids By Liquid Chromatography Tandem Mass Spectrometry (Lc-Ms/Ms), Tebah N. Browne
Semi-Quantitative Detection Of Signature Peptides In Body Fluids By Liquid Chromatography Tandem Mass Spectrometry (Lc-Ms/Ms), Tebah N. Browne
Student Theses
This study covers a modified semi-quantitative approach for liquid chromatography mass spectrometry (LC-MS/MS) signature peptide detection for body fluid identification. Peptide concentrations were measured based on synthetic peptide standards. Samples were processed with varying trypsin digestion and purification protocols, including a three-hour trypsin digestion and Microcon membrane filtration. The Microcon filtration method generates polymerase chain reaction (PCR) compatible DNA and peptide fractions that can be analyzed without any further purification. Preliminary validation tests covered stains on different substrates, semen/ saliva mixtures, minimum sample volume, and repeatability. All signature peptides in the multiplex were present at different concentrations and varied amongst …