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Full-Text Articles in Social and Behavioral Sciences
Development Of A Loop-Mediated Isothermal Amplification (Lamp) Assay For Detection Of Mirnas, Alisha B. Whelan
Development Of A Loop-Mediated Isothermal Amplification (Lamp) Assay For Detection Of Mirnas, Alisha B. Whelan
Master of Science in Forensic Science Directed Research Projects
Body fluid identification serves a vital role in contextualizing biological evidence received in forensic laboratories. While several fluids can be presumptively detected using immunological or catalytic tests, these tests are susceptible to false results and have limited sensitivity. True confirmatory testing is still somewhat limited in forensic laboratories. With the advancement of DNA typing technologies, laboratories can develop DNA profiles from trace amounts of sample, increasing the demand for sensitive and accurate body fluid detection. This project proposes using loop-mediated isothermal amplification (LAMP) as a method to rapidly detect microRNAs (miRNAs) that are differentially expressed in various body fluids with …
Optimization And Comparison Of Methods For Separation Of Spermatozoa From Superabsorbent Polymer-Containing Forensic Evidence, Hannah Wells
Optimization And Comparison Of Methods For Separation Of Spermatozoa From Superabsorbent Polymer-Containing Forensic Evidence, Hannah Wells
Master of Science in Forensic Science Directed Research Projects
Sexual assault is currently one of the most prevalent crimes that affects victims of all ages. Forensic DNA analysts often confirm proof of contact in sexual assault cases through the identification of spermatozoa and subsequent STR profiling. Diapers and other feminine hygiene products, such as maxi pads and ultrathin pads, are types of superabsorbent polymer-containing evidence that complicate the process of DNA analysis due to the trapping of the spermatozoa in the gel-like matrix. In this study, a comparison of methods was performed to determine which extraction technique produces the highest sperm cell and DNA yield and generates a usable …
Impact Of 1,4-Dithiothreitol (Dtt) On Microbial Community Structures Associated With Human Semen Samples, Christian R. Blaise
Impact Of 1,4-Dithiothreitol (Dtt) On Microbial Community Structures Associated With Human Semen Samples, Christian R. Blaise
Master of Science in Forensic Science Directed Research Projects
Body fluid identification (BFID) is one of the most important steps in forensic science as it provides important context to how DNA analysis should be performed on biological samples found at the scene of a crime. Many scientific methods on BFID have been developed over the years with respective benefits and flaws. One emerging method in recent years is microbial signature based BFID. This method uses the same DNA extract that is utilized for human DNA profile generation for microbial analysis. Although DNA is extracted from all body fluid samples without any additional steps, extraction of human DNA from semen …