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Social and Behavioral Sciences Commons™
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Full-Text Articles in Social and Behavioral Sciences
Analysis Of Forensic Relevant Cell Mixtures Using Cellular Autofluorescence And Fluorescence Activated Cell Sorting (Facs), Shayla Smith
Analysis Of Forensic Relevant Cell Mixtures Using Cellular Autofluorescence And Fluorescence Activated Cell Sorting (Facs), Shayla Smith
Master of Science in Forensic Science Directed Research Projects
Biological mixtures containing DNA from more than one contributor can make DNA profile interpretation challenging, especially for ‘touch’ or trace biological evidence. One approach to improve this is by differentiating cell populations from separate individuals prior to DNA profiling by utilizing intrinsic features of cells such as size, shape, and biochemical profiles. The goal of this study was to develop a new method for detecting and separating contributor populations in biological mixture samples using flow cytometry, a rapid and non-destructive approach with an unexplored potential for forensic casework. To accomplish this, two different mixture systems were investigated (1) mixtures containing …
Comparison Of Cell Counts To Dna Yield In Mock Biological Evidence Samples, Jacob Sewell
Comparison Of Cell Counts To Dna Yield In Mock Biological Evidence Samples, Jacob Sewell
Master of Science in Forensic Science Directed Research Projects
The implementation of rapid DNA analysis systems is of interest to forensic DNA casework, as this technology exhibits a sample-to-profile time of approximately two hours with limited handling of samples. This decreases the likelihood of contamination, and can potentially reduce DNA backlogs; however, current rapid DNA analysis systems do not execute one crucial step of the forensic DNA analysis workflow: human DNA quantitation. This step is necessary for DNA profiles to be compliant with the FBI’s Quality Assurance Standard 9.4, allowing DNA profiles to be uploaded and searched within the CODIS database. Coupling traditional qPCR methods with rapid DNA analysis …