Social and Behavioral Sciences Commons^™

Field Testing Of Collection Cards For Cannabis Sativa Samples With A Single Hexanucleotide Dna Marker, Lindsey Allgeier, John Hemenway, Nicholas Shirley, Tommy Lanier, Heather Miller Coyle

Forensic Science Publications

Abstract:  The validity and feasibility of using DNA collection cards in the field for preservation and analysis of Cannabis sativa genotypes were investigated using a highly specific hexanucleotide marker. Collection cards were submitted to the National Marijuana Initiative, which selectively trained and managed the collection of specific types of samples from a variety of participating agencies. Samples collected at seizure sites included fresh marijuana leaf samples, dried “dispensary” samples, U.S. border seizures, and hashish. Using a standardized PCR kit with custom-labeled oligonucleotide primers specific to marijuana, collection cards produced eight genotypes and 13 different alleles, extremely low baselines, and no …