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Social and Behavioral Sciences Commons

Open Access. Powered by Scholars. Published by Universities.®

Legal Studies

City University of New York (CUNY)

Student Theses

2020

Animal bones

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The Application Of A Simple Tissue-Disruption Method To Dna Extraction For Species Identification, Jordana R. Fox Aug 2020

The Application Of A Simple Tissue-Disruption Method To Dna Extraction For Species Identification, Jordana R. Fox

Student Theses

The TissueLyser II was used to disrupt animal bones and plant tissue without dry ice or liquid nitrogen for species identification. Bone fragments of fresh swine (Sus scrofa) ribs were used. A 521bp fragment of the cyt b locus was analyzed, and the BLASTn results confirmed that the origin of all the samples was swine (E-value = 0.0). Bone fragments of bovine, chicken, duck, fish, sheep, and swine were also used to test the procedure. An approximately 600bp fragment of the COI locus was analyzed, and the BLASTn results confirmed the origin of all the samples (E-value = …