Social and Behavioral Sciences Commons^™

Coomassie Brilliant Blue Dye As A Method For Analyzing Fracture Markings In Bone, Abigail Hoffmeister, David Harutunyan, Matthew Aizawa, Everett Baker, Brandon Mendoza, Chase Freeman, Siran Iskanian

Seaver College Research And Scholarly Achievement Symposium

Coomassie Brilliant Blue Dye is a dye commonly used to stain proteins. Because of its ability to adhere to proteins, this research has focused on perfecting a method of dyeing a fractured flat bone in order to most accurately observe and analyze fracture markings within the trabecular layer. Stereoscopic microscopy was the chosen technique of analysis for this research because of its proven effectiveness in glass and ceramic fractography to observe varying depths. In order to most effectively apply stereoscopic microscopy to this research, the following variables were manipulated to maximize color contrast in the trabecular layer in order to …

Extraction Of Challenging Forensic Samples Using The Microgem Dna Extraction Kit, Falyn R. Vega

Student Theses

In forensic science, DNA extraction can be a tedious and resource-intensive process. Extraction with Proteinase K is an industry standard but has its drawbacks, such as requiring multiple ionic detergents and washing steps. MicroGEM has developed a new enzyme called forensicGEM that is temperature-dependent and compatible with mesophilic enzymes, offering complete DNA extraction in about 20 minutes in a single tube, limiting contamination, loss of sample, and working time. ForensicGEM can extract DNA from highly degraded samples, potentially leading to more complete STR profiles. Highly degraded tissue and bone samples were collected and extracted with the forensicGEM …

Reply To Response By Fbi Laboratory Filed In Illinois V. Winfield And Affidavit By Biederman Et Al. (2022) Filed In Us V. Kaevon Sutton (2018 Cf1 009709), Susan Vanderplas, Kori Khan, Heike Hofmann, Alicia Carriquiry

Department of Statistics: Faculty Publications

1 Preliminaries

1.1 Scope

The aim of this document is to respond to issues raised in Federal Bureau of Investigation¹ and Alex Biedermann, Bruce Budowle & Christophe Champod.²

1.2 Conflict of Interest

We are statisticians employed at public institutions of higher education (Iowa State University and University of Nebraska, Lincoln) and have not been paid for our time or expertise when preparing either this response or the original affidavit.³ We provide this information as a public service and as scientists and researchers in this area.

1.3 Organization

The rest of the document precedes as follows: we begin …

Firearms And Toolmark Error Rates, Susan Vanderplas, Kori Khan, Heike Hofmann, Alicia L. Carriquiry

Department of Statistics: Faculty Publications

We have outlined several problems with the state of error rate studies on firearm and toolmark examination. Fundamentally, we do not know what the error rate is for these types of comparisons. This is a failure of the scientific study of toolmarks, rather than the examiners themselves, but until this is corrected with multiple studies that meet the criteria described in Section 3, we cannot support the use of this evidence in criminal proceedings.

Anatomical Diagrams And Dolls: Guidelines For Their Usage In Forensic Interviews And Courts Of Law, Victor Vieth

Mitchell Hamline Law Review

No abstract provided.

Developing A Maceration Method For Isolation Of Dna From Cancellous Bones, Bryan Bernal

Student Theses

Prior to isolating DNA, it is necessary to remove any biological materials that may be present on commingled remains. Manual maceration of the outer surface is time-consuming. This study will address this issue by developing a simple method for processing bone samples prior to DNA isolation. A liquid-based technique was applied to macerate bone fragments by incubating bone fragments in an enzyme solution, removing potential contaminants, followed by a bone tissue disruption. Swine (Sus scrofa) bones were used to simulate human bones. Microscopic analysis suggested this method was effective for surface material removal. The methods effect on the …

Semi-Quantitative Detection Of Signature Peptides In Body Fluids By Liquid Chromatography Tandem Mass Spectrometry (Lc-Ms/Ms), Tebah N. Browne

Student Theses

This study covers a modified semi-quantitative approach for liquid chromatography mass spectrometry (LC-MS/MS) signature peptide detection for body fluid identification. Peptide concentrations were measured based on synthetic peptide standards. Samples were processed with varying trypsin digestion and purification protocols, including a three-hour trypsin digestion and Microcon membrane filtration. The Microcon filtration method generates polymerase chain reaction (PCR) compatible DNA and peptide fractions that can be analyzed without any further purification. Preliminary validation tests covered stains on different substrates, semen/ saliva mixtures, minimum sample volume, and repeatability. All signature peptides in the multiplex were present at different concentrations and varied amongst …

Map My Murder: A Digital Forensic Study Of Mobile Health And Fitness Applications, Courtney Hassenfeldt, Shabana Baig, Ibrahim Baggili, Xiaolu Zhang

Electrical & Computer Engineering and Computer Science Faculty Publications

The ongoing popularity of health and fitness applications catalyzes

the need for exploring forensic artifacts produced by them. Sensitive

Personal Identifiable Information (PII) is requested by the applications

during account creation. Augmenting that with ongoing

user activities, such as the user’s walking paths, could potentially

create exculpatory or inculpatory digital evidence. We conducted

extensive manual analysis and explored forensic artifacts produced

by (n = 13) popular Android mobile health and fitness applications.

We also developed and implemented a tool that aided in the timely

acquisition and identification of artifacts from the examined applications.

Additionally, our work explored the type of …

Optical Tweezers As An Effective Tool For Spermatozoa Isolation From Mixed Forensic Samples, Nicole Auka, Michael Valle, Bobby D. Cox, Peter D. Wilkerson, Tracey Dawson Cruz, Joseph E. Reiner, Sarah J. Seashols-Williams

Forensic Science Publications

A single focus optical tweezer is formed when a laser beam is launched through a high numerical aperture immersion objective. This objective focuses the beam down to a diffraction-limited spot, which creates an optical trap where cells suspended in aqueous solutions can be held fixed. Spermatozoa, an often probative cell type in forensic investigations, can be captured inside this optical trap and dragged one by one across millimeter-length distances in order to create a cluster of cells which can be subsequently drawn up into a capillary for collection. Sperm cells are then ejected onto a sterile cover slip, counted, and …

Compound-Specific Isotope Analysis Of Amino Acids In Biological Tissues: Applications In Forensic Entomology, Food Authentication And Soft-Biometrics In Humans, Mayara Patricia Viana De Matos

Graduate Theses, Dissertations, and Problem Reports

In this work we demonstrate the power of compound-specific isotope analysis (CSIA) to analyze proteinaceous biological materials in three distinct forensic applications, including: 1) linking necrophagous blow flies in different life stages to their primary carrion diet; 2) identifying the harvesting area of oysters for food authentication purposes; and 3) the ability to predict biometric traits about humans from their hair.

In the first application, we measured the amino-acid-level fractionation that occurs at each major life stage of Calliphora vicina (Robineau-Desvoidy) (Diptera: Calliphoridae) blow flies. Adult blow flies oviposited on raw pork muscle, beef muscle, or chicken liver. Larvae, pupae …

Furthering Understanding Of Forensic Units: A Detailed Examination Of Knoxville Police Department's Crime Scene Unit., Cassandra Christina Rausch

Electronic Theses and Dissertations

Throughout the past three decades, the criminal justice system has decidedly employed new technologies for the purposes of establishing guilt or innocence. Forensic science, with its synthesis of scientific methodology and investigative considerations of law enforcement, ushered in the development of DNA profiling and forever changed the process of crime scene investigation.This shift in focus also led to a shift in the individuals involved in investigation, producing the widespread formation of stand-alone crime scene units. Utilizing both civilian and sworn employees of a law enforcement agency, these units became responsible for the documentation, collection, and preservation of evidence that would …

Digital Forensics In The Next Five Years, Laoise Luciano, Ibrahim Baggili, Mateusz Topor, Peter Casey, Frank Breitinger

Electrical & Computer Engineering and Computer Science Faculty Publications

Cyber forensics has encountered major obstacles over the last decade and is at a crossroads. This paper presents data that was obtained during the National Workshop on Redefining Cyber Forensics (NWRCF) on May 23-24, 2017 supported by the National Science Foundation and organized by the University of New Haven. Qualitative and quantitative data were analyzed from twenty-four cyber forensics expert panel members. This work identified important themes that need to be addressed by the community, focusing on (1) where the domain currently is; (2) where it needs to go and; (3) steps needed to improve it. Furthermore, based on the …

Forensic Analysis Of Immersive Virtual Reality Social Applications: A Primary Account, Ananya Yarramreddy, Peter Gromkowski, Ibrahim Baggili

Electrical & Computer Engineering and Computer Science Faculty Publications

Our work presents the primary account for exploring the forensics of immersive Virtual Reality (VR) systems and their social applications. The Social VR applications studied in this work include Bigscreen, Altspace VR, Rec Room and Facebook Spaces. We explored the two most widely adopted consumer VR systems: the HTC Vive and the Oculus Rift. Our tests examined the efficacy of reconstructing evidence from network traffic as well as the systems themselves. The results showed that a significant amount of forensically relevant data such as user names, user profile pictures, events, and system details may be recovered. We anticipate that this …

Experience Constructing The Artifact Genome Project (Agp): Managing The Domain's Knowledge One Artifact At A Time, Cinthya Grajeda, Laura Sanchez, Ibrahim Baggili, Devon R. Clark, Frank Breitinger

Electrical & Computer Engineering and Computer Science Faculty Publications

While various tools have been created to assist the digital forensics community with acquiring, processing, and organizing evidence and indicating the existence of artifacts, very few attempts have been made to establish a centralized system for archiving artifacts. The Artifact Genome Project (AGP) has aimed to create the largest vetted and freely available digital forensics repository for Curated Forensic Artifacts (CuFAs). This paper details the experience of building, implementing, and maintaining such a system by sharing design decisions, lessons learned, and future work. We also discuss the impact of AGP in both the professional and academic realms of digital forensics. …

Exploring Various Techniques To Process And Identify Latent Friction Ridge Details From Fingers On Semi-Porous Surfaces, Emily Campo

Honors Theses

Numerous terrorist groups in the Middle East have been recently making detonators for IEDs (Improvised Explosive Devices) using items as basic as flip flops. Because of the semi-porous nature of flip flops, military forensic response teams have been unable to find an effective method through which they can identify, process, and lift latent fingerprints from the surface of a flip flop. The present study aims to use three fingerprint principles to formulate a reliable method that can be used for such purposes. This study specifically investigated the potential use of powders and cyanoacrylate fuming as processing techniques. The case study …

Forensic Markers Of Lampricide Toxicity In Oncorhynchsus Mykiss, Chris White

Theses and Dissertations (Comprehensive)

The lampricide 3-trifluoromethyl-4-nitrophenol (TFM) has been used for decades to control invasive sea lamprey (Petromyzon marinus) populations in the Great Lakes, normally with little harm to other fish populations. However, due to the nature of lampricide treatments and the chemical properties of TFM, adverse effects to non-target fishes and non-target mortality occasionally occur. However, investigations of non-target mortality can be complex, particularly if fish deaths are not noticed immediately, due to decomposition of the carcasses. The objectives of this thesis were to expose rainbow trout (Oncorhynchsus mykiss) to TFM in order to: (i) identify forensic markers …

Forensic Linguist Tej Bhatia On The Hunt For The Unabomber, Tej K. Bhatia

Languages, Literatures, and Linguistics - All Scholarship

From article: [Editor’s note: Discovery Channel’s new 8-part series MANHUNT: UNABOMBER is about the FBI investigator Jim Fitzgerald who identified the Unabomber as Ted Kaczynski based on his writings–pioneering the use of what is now called forensic linguistics. The series stars Sam Worthington and Paul Bettany, and also features Chris Noth, Elizabeth Reaser, and Brian F. O’Byrne. Science & Film commissioned Syracuse University linguistics professor Dr. Tej Bhatia to write about the case. The series is available via Netflix and Amazon.]

Breaking Into The Vault: Privacy, Security And Forensic Analysis Of Android Vault Applications, Xiaolu Zhang, Ibrahim Baggili, Frank Breitinger

Electrical & Computer Engineering and Computer Science Faculty Publications

In this work we share the first account for the forensic analysis, security and privacy of Android vault applications. Vaults are designed to be privacy enhancing as they allow users to hide personal data but may also be misused to hide incriminating files. Our work has already helped law enforcement in the state of Connecticut to reconstruct 66 incriminating images and 18 videos in a single criminal case. We present case studies and results from analyzing 18 Android vault applications (accounting for nearly 220 million downloads from the Google Play store) by reverse engineering them and examining the forensic artifacts …

The Optimization Of Pressure Cycling Technology (Pct) For Differential Extraction Of Sexual Assault Casework, Vanessa Martinez

FIU Electronic Theses and Dissertations

A two-step protocol has been devised as a rapid and selective alternative to conventional differential extraction techniques with an increased recovery of DNA. The protocol involves pressure cycling with the Barocycler^® NEP 2320 from Pressure Biosciences. Inc. in alkaline conditions for epithelial cell lysis and removal. This step is followed by alkaline lysis at 95^º C for extraction of sperm cell DNA. At 1:1 or 2:1 female to male cell ratios, high selectivity and complete separation can be achieved. But at higher ratios, male allelic dropout is observed. This protocol has been modified to generate a clean male …

Fors 401: Forensic Biochemistry—A Peer Review Of Teaching Project Inquiry Portfolio, Ashley Hall

UNL Faculty Course Portfolios

Students who participate in active and experiential learning activities develop a better understanding of basic scientific principles and are also more likely to be retained in a STEM discipline. Developing such activities, however, can be difficult. In the present study, we sought to develop classroom activities to engage university students and increase understanding of the theory and practice of forensic science. One common accessory amongst the target audience was the ever-present media, both social and popular (entertainment). In fact, a majority of students report watching at least one of the many popular forensic science shows on TV. Therefore, we hypothesized …

A Historical Review And Resource Guide To The Scholarship Of Teaching And Training In Psychology And Law And Forensic Psychology, Cynthia J. Najdowski, Bette L. Bottoms, Margaret C. Stevenson, Jennifer C. Veilleux

Psychology Faculty Scholarship

The field of psychology and law, including forensic psychology, is an exciting concentration of research activity and student training and has grown rapidly, but to what extent have teaching and training efforts in the field been systematically catalogued and evaluated? We conducted a historical review and content analysis of the American and Canadian literature on the scholarship of teaching and training in the field. This review catalogs (a) information related to the development of training and teaching, (b) descriptions of programs and courses at various levels, and (c) articles on teaching resources or techniques in this field. We hope it …

Identification Of Characteristic Volatile Organic Compounds Released During The Decomposition Process Of Human Remains And Analogues, Norma Iris Caraballo

FIU Electronic Theses and Dissertations

The manner in which remains decompose has been and is currently being researched around the world, yet little is still known about the generated scent of death. In fact, it was not until the Casey Anthony trial that research on the odor released from decomposing remains, and the compounds that it is comprised of, was brought to light. The Anthony trial marked the first admission of human decomposition odor as forensic evidence into the court of law; however, it was not “ready for prime time” as the scientific research on the scent of death is still in its infancy.

This …

Evidentiary Power And Propriety Of Digital Identifiers And The Impact On Privacy Rights In The United States, Michael Losavio, Deborah Keeling

Journal of Digital Forensics, Security and Law

Media and network systems capture and store data about electronic activity in new, sometimes unprecedented ways; computational systems make for new means of analysis and knowledge development. These new forms offer new, powerful tactical tools for investigations of electronic malfeasance under traditional legal regulation of state power, particular that of Fourth Amendment limitations on police searches and seizures under the U.S. Constitution. But autonomy, identity and authenticity concerns with electronic data raise issues of public policy, privacy and proper police oversight of civil society. We examine those issues and their implications for digital and computational forensics

"It's Not About You": Exploring The Liminal Experiences Of Graduate Forensic Coaches, Christopher Paul Outzen

All Graduate Theses, Dissertations, and Other Capstone Projects

The following document is a capstone thesis project focusing on the unique experiences of graduate forensic coaches through lens of liminality, a performance theory used to describe a sense of being between social identities when going through a rite of passage. The author contends that this liminal experience has unique characteristics which are important to consider in the context of identity and forensic culture. In order to gather data, the author utilized qualitative, semi-structured interviews with current graduate forensic coaches. The resulting interviews were interpreted using a process of open coding to determine key themes of the experience. The author …

Exploring Forensic Implications Of The Fusion Drive, Shruti Gupta, Marcus Rogers

Journal of Digital Forensics, Security and Law

This paper explores the forensic implications of Apple’s Fusion Drive. The Fusion Drive is an example of auto-tiered storage. It uses a combination of a flash drive and a magnetic drive. Data is moved between the drives automatically to maximize system performance. This is different from traditional caches because data is moved and not simply copied. The research included understanding the drive structure, populating the drive, and then accessing data in a controlled setting to observe data migration strategies. It was observed that all the data is first written to the flash drive with 4 GB of free space always …

Relating Admissibility Standards For Digital Evidence To Attack Scenario Reconstruction, Changwei Liu, Anoop Singhal, Duminda Wijesekera

Journal of Digital Forensics, Security and Law

Attackers tend to use complex techniques such as combining multi-step, multi-stage attack with anti-forensic tools to make it difficult to find incriminating evidence and reconstruct attack scenarios that can stand up to the expected level of evidence admissibility in a court of law. As a solution, we propose to integrate the legal aspects of evidence correlation into a Prolog based reasoner to address the admissibility requirements by creating most probable attack scenarios that satisfy admissibility standards for substantiating evidence. Using a prototype implementation, we show how evidence extracted by using forensic tools can be integrated with legal reasoning to reconstruct …

Effects Of The Factory Reset On Mobile Devices, Riqui Schwamm, Neil C. Rowe

Journal of Digital Forensics, Security and Law

Mobile devices usually provide a “factory-reset” tool to erase user-specific data from the main secondary storage. 9 Apple iPhones, 10 Android devices, and 2 BlackBerry devices were tested in the first systematic evaluation of the effectiveness of factory resets. Tests used the Cellebrite UME-36 Pro with the UFED Physical Analyzer, the Bulk Extractor open-source tool, and our own programs for extracting metadata, classifying file paths, and comparing them between images. Two phones were subjected to more detailed analysis. Results showed that many kinds of data were removed by the resets, but much user-specific configuration data was left. Android devices did …

Forensic Science Forum, San Jose State University

Forensic Science Forum (Justice Studies)

No abstract provided.

Development Of Micro Volume Dna And Rna Profiling Assays To Identify The Donor And Tissue Source Of Origin Of Trace Forensic Biological Evidence, Brittany Morgan

Electronic Theses and Dissertations

In forensic casework analysis it is necessary to obtain genetic profiles from increasingly smaller amounts of biological material left behind by perpetrators of crime. The ability to obtain profiles from trace biological evidence is demonstrated with so-called ‘touch DNA evidence’ which is perceived to be the result of DNA obtained from shed skin cells transferred from donor to an object or person during physical contact. However, the current method of recovery of trace DNA involves cotton swabs or adhesive tape to sample an area of interest. This "blindswabbing" approach may result in the recovery of biological material from different individuals …

Seeing Is Believing: The Csi Effect Among Jurors In Malicious Wounding Cases, Corey Call, Amy K. Cook, John D. Reitzel, Robyn D. Mcdougle

Journal of Social, Behavioral, and Health Sciences

With the popularization of television crime shows that focus heavily on forensic science, such as CSI and its spin-offs, concerns about a new threat to jury trials have emerged in recent years. Dubbed the “CSI effect,” this phenomenon has reportedly come to influence the way jurors perceive forensic evidence at trials based on the way forensic evidence is presented on television. While the CSI effect has been the topic of much discussion throughout the popular press, the CSI effect has seldom been empirically tested. In this study, we present a selection of media accounts as well as criminological and …