Environmental Health and Protection Commons^™

Development And Evaluation Of A Quantitative Pcr Assay Targeting Sandhill Crane (Grus Canadensis) Fecal Pollution, Hodon Ryu, Jingrang Lu, Jason Vogel, Michael Elk, Felipe Chávez-Ramírez, Nicholas Ashbolt, Jorge Santo Domingo

United States Environmental Protection Agency: Staff Publications

While the microbial water quality in the Platte River is seasonally impacted by excreta from migrating cranes, there are no methods available to study crane fecal contamination. Here we characterized microbial populations in crane feces using phylogenetic analysis of 16S rRNA gene fecal clone libraries. Using these sequences, a novel crane quantitative PCR (Crane1) assay was developed, and its applicability as a microbial source tracking (MST) assay was evaluated by determining its host specificity and detection ability in environmental waters. Bacteria from crane excreta were dominated by bacilli and proteobacteria, with a notable paucity of sequences homologous to Bacteroidetes and …