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Oceanography and Atmospheric Sciences and Meteorology

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University of Nebraska - Lincoln

Microsatellites

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Articles 1 - 4 of 4

Full-Text Articles in Geology

Evidence For Recent Population Bottlenecks In Northern Spotted Owls (Strix Occidentalis Caurina), W. Chris Funk, Eric D. Forsman, Matthew Johnson, Thomas D. Mullins, Susan M. Haig Jan 2010

Evidence For Recent Population Bottlenecks In Northern Spotted Owls (Strix Occidentalis Caurina), W. Chris Funk, Eric D. Forsman, Matthew Johnson, Thomas D. Mullins, Susan M. Haig

United States Geological Survey: Staff Publications

The northern spotted owl (Strix occidentalis caurina) is one of the most controversial threatened subspecies ever listed under the US Endangered Species Act. Despite protection of its remaining forest habitat, recent field studies show continued declines of northern spotted owls. One potential threat to northern spotted owls which has not yet been shown is loss of genetic variation from population bottlenecks. Bottlenecks can increase the probability of mating among related individuals, potentially causing inbreeding depression, and can decrease adaptive potential. Here we report evidence for recent bottlenecks in northern spotted owls using a large genetic dataset (352 individuals …


Introgression And Dispersal Among Spotted Owl (Strix Occidentalis) Subspecies, W. Chris Funk, Eric D. Forsman, Thomas D. Mullins, Susan M. Haig Jan 2008

Introgression And Dispersal Among Spotted Owl (Strix Occidentalis) Subspecies, W. Chris Funk, Eric D. Forsman, Thomas D. Mullins, Susan M. Haig

United States Geological Survey: Staff Publications

Population genetics plays an increasingly important role in the conservation and management of declining species, particularly for defining taxonomic units. Subspecies are recognized by several conservation organizations and countries and receive legal protection under the US Endangered Species Act (ESA). Two subspecies of spotted owls, northern (Strix occidentalis caurina) and Mexican (S. o. lucida) spotted owls, are ESA-listed as threatened, but the California (S. o. occidentalis) spotted owl is not listed. Thus, determining the boundaries of these subspecies is critical for effective enforcement of the ESA. We tested the validity of previously recognized spotted …


Microsatellite Loci For Distinguishing Spotted Owls (Strix Occidentalis), Barred Owls (Strix Varia), And Their Hybrids, W. Chris Funk, Thomas D. Mullins, Eric D. Forsman, Susan M. Haig Jan 2007

Microsatellite Loci For Distinguishing Spotted Owls (Strix Occidentalis), Barred Owls (Strix Varia), And Their Hybrids, W. Chris Funk, Thomas D. Mullins, Eric D. Forsman, Susan M. Haig

United States Geological Survey: Staff Publications

We identified four diagnostic microsatellite loci that distinguish spotted owls (Strix occidentalis), barred owls (Strix varia), F1 hybrids and backcrosses. Thirty-four out of 52 loci tested (65.4%) successfully amplified, and four of these loci (11.8%) had allele sizes that did not overlap between spotted and barred owls. The probability of correctly identifying a backcross with these four loci is 0.875. Genotyping potential hybrid owls with these markers revealed that field identifications were often wrong. Given the difficulty of identifying hybrids in the field, these markers will be useful for hybrid identification, law enforcement and spotted …


Conservation Genetics Of Snowy Plovers (Charadrius Alexandrinus) In The Western Hemisphere: Population Genetic Structure And Delineation Of Subspecies, W. Chris Funk, Thomas D. Mullins, Susan M. Haig Jan 2007

Conservation Genetics Of Snowy Plovers (Charadrius Alexandrinus) In The Western Hemisphere: Population Genetic Structure And Delineation Of Subspecies, W. Chris Funk, Thomas D. Mullins, Susan M. Haig

United States Geological Survey: Staff Publications

We examined the genetic structure of snowy plovers (Charadrius alexandrinus) in North America, the Caribbean, and the west coast of South America to quantify variation within and among breeding areas and to test the validity of three previously recognized subspecies. Sequences (676 bp) from domains I and II of the mitochondrial control region were analyzed for 166 snowy plovers from 20 breeding areas. Variation was also examined at 10 microsatellite loci for 144 snowy plovers from 14 breeding areas. The mtDNA and microsatellite data provided strong evidence that the Puerto Rican breeding group is genetically divergent from sites …