Physical Sciences and Mathematics Commons^™

Can Desert Mosses Hide From Climate Change? The Ecophysiological Importance Of Habitat Buffering & Water Relations To A Keystone Biocrust Moss In The Mojave Desert, Theresa Ann Clark

UNLV Theses, Dissertations, Professional Papers, and Capstones

Empirical and observational studies suggest a keystone biocrust moss, Syntrichia caninervis, may be sensitive to future climate change in the American Southwest due to its uniquely sensitive water relations that appear particularly challenged during summer hydration-desiccation cycles. However, the potential mitigating roles of habitat buffering, acclimatization, and winter recovery on the vulnerability of this species remain largely unexplored. I investigated potential abiotic and biotic resiliency factors driving summer stress resistance and recovery in S. caninervis along present-day aridity gradients in the Mojave Desert to strengthen the climate change vulnerability assessment for this species common to biocrusts of North America, northern …

Single-Cell Growth Rates In Photoautotrophic Populations Measured By Stable Isotope Probing And Resonance Raman Microspectrometry, Gordon T. Taylor, Elizabeth A. Suter, Zhuo Q. Li, Stephanie Chow, Dallyce Stinton, Tatiana Zaliznyak, Steven R. Beaupre

Faculty Works: Biology, Chemistry, and Environmental Studies

A newmethod tomeasure growth rates of individual photoautotrophic cells by combining stable isotope probing (SIP) and single-cell resonance Raman microspectrometry is introduced. This report explores optimal experimental design and the theoretical underpinnings for quantitative responses of Raman spectra to cellular isotopic composition. Resonance Raman spectra of isogenic cultures of the cyanobacterium, Synechococcus sp., grown in 13C-bicarbonate revealed linear covariance between wavenumber (cm−1) shifts in dominant carotenoid Raman peaks and a broad range of cellular 13C fractional isotopic abundance. Single-cell growth rates were calculated from spectra-derived isotopic content and empirical relationships. Growth rates among any 25 cells in a sample varied …

Experimental Impacts Of Climate Warming And Ocean Carbonation On Eelgrass Zostera Marina, Richard C. Zimmerman, Victoria J. Hill, Malee Jinuntuya, Billur Celebi, David Ruble, Miranda Smith, Tiffany Cedeno, W. Mark Swingle

OES Faculty Publications

CO₂ is a critical and potentially limiting substrate for photosynthesis of both terrestrial and aquatic ecosystems. In addition to being a climate-warming greenhouse gas, increasing concentrations of CO₂ will dissolve in the oceans, eliciting both negative and positive responses among organisms in a process commonly known as ocean acidification. The dissolution of CO₂ into ocean surface waters, however, also increases its availability for photosynthesis, to which the highly successful, and ecologically important, seagrasses respond positively. Thus, the process might be more accurately characterized as ocean carbonation. This experiment demonstrated that CO₂ stimulation of primary production enhances …

Evolutionary Responses Of Invasive Grass Species To Variation In Precipitation And Soil Nitrogen, Monica A. Nguyen, Amy E. Ortega, Quoc L. Nguyen, Sarah Kimball, Michael L. Goulden, Jennifer L. Funk

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

1.Global climate models suggest that many ecosystems will experience reduced precipitation over the next century and the consequences for invasive plant performance are largely unknown. Annual invasive species may be able to quickly evolve traits associated with drought escape or tolerance through rapid genetic changes.

2.We investigated the influence of five years of water and nitrogen manipulations on trait values in a southern California grassland system. Seeds from two annual grass species (Avena barbata, Bromus madritensis) were collected from experimental plots and grown in a common environment over two generations. We measured 14 physiological, morphological, phenological, and …

Phosphorus Cycling In The Sargasso Sea: Investigation Using The Oxygen Isotopic Composition Of Phosphate, Enzyme-Labeled Fluorescence, And Turnover Times, Karen Mclaughlin, Jill A. Sohm, Gregory A. Cutter, Michael W. Lomas, Adina Paytan

OES Faculty Publications

Dissolved inorganic phosphorus (DIP) concentrations in surface water of vast areas of the ocean are extremely low (<10 nM) and phosphorus (P) availability could limit primary productivity in these regions. We explore the use of oxygen isotopic signature of dissolved phosphate (δ¹⁸O_PO4) to investigate biogeochemical cycling of P in the Sargasso Sea, Atlantic Ocean. Additional techniques for studying P dynamics including ³³P-based DIP turnover time estimates and percent of cells expressing alkaline phosphatase (AP) activity as measured by enzyme-labeling fluorescence are also used. In surface waters, δ¹⁸O_PO4 values were lower than equilibrium by 3–6%, indicative of dissolved organic phosphorous (DOP) remineralization by extracellular enzymes. An isotope mass balance model using a variety of possible combinations of …

Ecophysiology Of Two Native Invasive Woody Species And Two Dominant Warm-Season Grasses In The Semiarid Grasslands Of The Nebraska Sandhills, Kathleen D. Eggemeyer, Tala Awada, David A. Wedin, F. Edwin Harvey, Xinhua Zhou

School of Natural Resources: Faculty Publications

Populations of Pinus ponderosa and Juniperus virginiana are expanding into semiarid Sandhills grasslands in Nebraska. To evaluate the physiological basis of their success, we measured the seasonal course of leaf gas exchange, plant water status, and carbon isotope discrimination in these two native trees and two native C₄ grasses (Schizachyrium scoparium and Panicum virgatum). Compared to the trees, grasses had higher net photosynthetic rates (A_net) and water use efficiency (WUE) and more negative predawn and midday water potentials (Ψ) in June and July. While leaf Ψ and rates of leaf gas exchange declined for all …

Relationships Between Needle Nitrogen Concentration And Photosynthetic Responses Of Douglas-Fir Seedlings To Elevated Co2 And Temperature, James D. Lewis, Melissa S. Lucash, David M. Olszyk, David T. Tingey

Environmental Science and Management Faculty Publications and Presentations

• Here we examined correlations between needle nitrogen concentration ([N]) and photosynthetic responses of Douglas-fir (Pseudotsuga menziesii) seedlings to growth in elevated temperatures and atmospheric carbon dioxide concentrations ([CO₂]).
• Seedlings were grown in sunlit, climate-controlled chambers at ambient or ambient+3.5^° C and ambient or ambient +180 μmol mol^-1 CO₂ in a full factorial design. Photosynthetic parameters and needle [N] were measured six times over a 21-month period.
• Needle [N] varied seasonally, and accounted for 30–50% of the variation in photosynthetic parameters. Across measurement periods, elevated temperature increased needle [N] by 26% and light-saturated …

Stomatal Responses Of Douglas-Fir Seedlings To Elevated Carbon Dioxide And Temperature During The Third And Fourth Years Of Exposure, James D. Lewis, Melissa S. Lucash, David M. Olszyk, David T. Tingey

Environmental Science and Management Faculty Publications and Presentations

Two major components of climate change, increasing atmospheric [CO₂] and increasing temperature, may substantially alter the effects of water availability to plants through effects on the rate of water loss from leaves. We examined the interactive effects of elevated [CO₂] and temperature on seasonal patterns of stomatal conductance (g_s), transpiration (E) and instantaneous transpiration efficiency (ITE) in Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) seedlings. Seedlings were grown in sunlit chambers at either ambient CO₂ (AC) or ambient + 180 µmol mol^-1 CO₂ (EC), and at ambient temperature (AT) or ambient + 3.5° …

Effect Of Light/Dark Transition On Carbon Translocation In Eelgrass Zostera Marina Seedlings, Richard C. Zimmerman, Randall S. Alberte

OES Faculty Publications

Carbon translocation in the marine macrophyte Zostera marina L. (eelgrass) was investigated to elucidate the impact of light/dark transitions on sucrose partitioning between roots and shoots. After exposure of leaves to C-14-bicarbonate, the level of C-14-labelled photoassimilates increased monotonically in both leaves and fully aerobic roots of plants maintained in the light. Accumulation of C-14 in roots and leaves ceased abruptly when plants were transferred to darkness that induced root anaerobiosis even though C-14 levels remained high in the dark-exposed leaves. Thus, translocation of C-14 photoassimilates from shoots to roots was inhibited when roots became anoxic. Anoxia induced by light …

The Photosynthetic Action Spectra Of The Phytoplankton And Their Role In Governing Spatial And Temporal Distribution: A Numerical Modeling Approach, William Morrow Willis

OES Theses and Dissertations

In the present study a numerical modeling approach is employed to examine the role of division spedific differences in photosynthetic action spectra in governing the relative size of diatom and dinoflagellate carbon synthesis along various marine light regime gradients. A radiative transfer model taking into account both Rayleigh and Mie atmospheric optical properties is employed to define the light regime incident on the sea surface. The hydrospheric light regime is defined by an exponential decay model with a correction for diffuse back scatter. Taken together, the atmospheric and hydrospheric models define the spectral composition and intensity of light in the …

Seasonal And Spatial Variation In Primary Productivity In Boulder Basin, Lake Mead, Clark County, Nevada, Isamu Aoki

Publications (WR)

The ¹⁴C light and dark bottle technique for measurement of primary production was utilized as a means of assessing the amount of inorganic carbon being converted Into organic form by the photosynthesis of phytoplankton populations In the Boulder Basin of Lake Mead.

Spatial and time series changes of productivity levels observed at eight sampling locations within Boulder Basin Indicate that the Influence of treated municipal arts' industrial effluent flowing into Les Vegas Bay is contributing high levels of available nutrients at Las Vegas Wash Inlet to cause productivity to approximate those levels associated with polluted waters.

Productivity levels at …

Field Measurements Of Photosynthesis And Leaf Growth Rates Of Three Alpine Plant Species, Douglas A. Johnson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Leaf photosynthetic measurements using a portable ¹⁴Co₂ field system were carried out and correlative leaf relative growth rates, RGR, were determined at different leaf positions of three alpine plant species throughout the growing season. Initially there was a period of high leaf RGR associated with a period of increasing photosynthetic activity. Following this stage was a long period of no net change in length of the living leaf. During this period, photosynthetic activity generally increased to a maximum level and then decreased steadily. The final ontogenetic stage was a period of negative leaf RGR denoting leaf senescence which …