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Full-Text Articles in Physical Sciences and Mathematics
Inhibition Of Cellular Respiration By Doxorubicin, Zhimin Tao, Henry G. Withers, Harvey S. Penefsky, Jerry Goodisman, Abdul Kader Souid
Inhibition Of Cellular Respiration By Doxorubicin, Zhimin Tao, Henry G. Withers, Harvey S. Penefsky, Jerry Goodisman, Abdul Kader Souid
Chemistry - All Scholarship
Doxorubicin executes apoptosis, a process known to produce leakage of cytochrome c and opening of the mitochondrial permeability transition pores. To define the loss of mitochondrial function by apoptosis, we monitored cellular respiration during continuous exposure to doxorubicin. A phosphorescence analyzer capable of stable measurements over at least 5 h was used to measure [O(2)]. In solutions containing glucose and cells, [O(2)] declined linearly with time, showing that the kinetics of oxygen consumption was zero order. Complete inhibition of oxygen consumption by cyanide indicated that oxidations occurred in the respiratory chain. A decline in the rate of respiration was evident …
Activation Of Carboplatin By Carbonate, Anthony J. Di Pasqua, Jerry Goodisman, Deborah J. Kerwood, Bonnie B. Toms, James C. Dabrowiak
Activation Of Carboplatin By Carbonate, Anthony J. Di Pasqua, Jerry Goodisman, Deborah J. Kerwood, Bonnie B. Toms, James C. Dabrowiak
Chemistry - All Scholarship
Carboplatin, [Pt(NH3)2(CBDCA-O,O')], 1, where CBDCA is cyclobutane-1,1-dicarboxylate, is in wide clinical use for the treatment of ovarian, lung, and other types of cancer. Because carboplatin is relatively unreactive toward nucleophiles, an important question concerning the drug is the mechanism by which it is activated in vivo. Using [1H,15N] heteronuclear single quantum coherance spectroscopy (HSQC) NMR and 15N-labeled carboplatin, we show that carboplatin reacts with carbonate ion in carbonate buffer to produce ring-opened products, the nature of which depends on the pH of the medium. The assignment of HSQC NMR resonances was facilitated by studying the reaction of carboplatin in strong …
Cisplatin Carbonato Complexes. Implications For Uptake, Antitumor Properties, And Toxicity, Corey R. Centerwall, Jerry Goodisman, Deborah J. Kerwood, James C. Dabrowiak
Cisplatin Carbonato Complexes. Implications For Uptake, Antitumor Properties, And Toxicity, Corey R. Centerwall, Jerry Goodisman, Deborah J. Kerwood, James C. Dabrowiak
Chemistry - All Scholarship
The reaction of aquated cisplatin with carbonate which is present in culture media and blood is described. The first formed complex is a monochloro monocarbonato species, which upon continued exposure to carbonate slowly forms a biscarbonato complex. The formation of carbonato species under conditions that simulate therapy may have important implications for uptake, antitumor properties, and toxicity of cisplatin.