Physical Sciences and Mathematics Commons^™

A Kinetic Study Pertaining To The Enzymatic Reduction Of Pyruvate By Lactate Dehydrogenase, Ronald Floyd Tienhaara

All Master's Theses

The reduction of pyruvate as catalyzed by lactate dehydrogenase has been studied by monitoring the oxidation of DPNH (diphosphopyridine nucleotide) at 340 nm. The kinetic investigation was carried out in phosphate buffer at 25° C. Since pyruvate exists in equilibrium with the enolized and hydrated forms, a kinetic analysis was devised to determine the nature of the true substrate for the enzymatic process. Also, a comparative study was made involving two isozymes of lactate dehydrogenase. The catalytic effectiveness of these isozymes was studied as a function of pH and substrate concentration.

Synthesis And Characterization Of Some Dioxane Complexes, James Donald Satterlee

All Master's Theses

The boron trifluoride, tin (IV) fluoride and titanium (IV) fluoride complexes with 1,4-dioxane have been prepared and studied using infrared techniques. The compositions of the complexes have been determined and correspond to the 1:1 adducts in the case of the tin and titanium compounds. The boron trifluoride adduct is a 2:1 (acid:base) adduct. Infrared spectra in the region 1300 cm.-1 to 550 cm.-1 are given and the effects of coordination upon the ligand vibrations are described.

A Kinetic Analysis Of The Iodination Of Pyruvic Acid And Pyruvate Esters, Mohamed M. Fahmi Hegazi

All Master's Theses

In the present work the relative rates of iodination of pyruvic acid and ethyl and methyl pyruvate have been compared at 25.0° C. These studies allow the determination of the respective free energies of activation for these processes. Measurements have also been carried out at various temperatures to determine the enthalpies and entropies of activation for the spontaneous and base catalysed iodinations of these pyruvate systems. The present thesis also includes a comparison of the solvent deuterium isotope effects associated with the spontaneous rate of iodination of pyruvic acid and the alkyl pyruvates. Also, the catalytic rate coefficients associated with …

Palladium(Ii) And Platinum(Ii) Complexes Of 2-Cyanophenyl Diphenylphosphine, David Harrison Payne

University of the Pacific Theses and Dissertations

There has been no successful chelation of any phosphorus-acetylenic or phosphorous-nitrile ligand. The objectives of this study were to synthesize such ligands and to study their chemistry and chelating tendencies. The best choice appeared to be the preparation of ortho-substituted triphenylphosphines similar to the chelating ligand allylphenyldiphenylphosphine. The Grignard reactions utilized in the preparation of the olefinic compound could not be used in most cases due to the high reactivity of the acetylene and nitrile function Bromobenzene and iodobenzene have been reacted with alkali metal phosphines under conditions that were probably too drastic for the acetylenic and nitrile functions. There …

Fluorescence Lifetimes And Quantum Efficiencies Of Some 1,10-Phenanthrolines, Paul John Tabakian

University of the Pacific Theses and Dissertations

Fluorescence properties of fourteen substituted 1,10-Phenanthrolines were determined using two different solvent systems. Hour different sets of experiments were performed in order to measure fluorescence lifetimes, quantum efficiencies, absorption and corrected fluorescence spectra, oscillator strengths, and Stoke’s shifts. Two of the phenanthrolines, the 5-Nitro-1,10-Phenanthroline and the 1,10-Phenanthroline-5,6-Dione, did not fluoresce.

A new equation was developed in order to extract the true fluorescence lifetimes τ from the observed fluorescence output f(t), and the lamp decay function I(t). This equation was [see PDF] where “A” was the weight factor or the normalization constant for the appropriate functions. A test of this equation …