Physical Sciences and Mathematics Commons^™

Pilot Project For Future Fast And Accurate Detection Of The Human Fecal Marker Hf183 For Presence Of Human Pollution In The New River, Angie Louis

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One type of pollution that has a negative impact on the environmental waters originates from fecal contamination. Identifying the source of pollution is an important step in effective resource management, mitigation, and reducing risk to human health. Microbial source tracking (MST) can be used to identify fecal pollution and to identify specific microbes in the environment and the quantitative polymerase chain reaction (qPCR) can be utilized in this analysis. In this pilot study the New River waterway, running through the City of Fort Lauderdale, was tested for a human specific bacteria Bacteroids, using the TaqMan HF183 qPCR assay (HF183/BFDrev). …