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Full-Text Articles in Physical Sciences and Mathematics
Capturing Variation In Lens (Fabaceae): Development And Utility Of An Exome Capture Array For Lentil, Ezgi Ogutcen, Larissa Ramsay, Eric Bishop Von Wettberg, Kirstin E. Bett
Capturing Variation In Lens (Fabaceae): Development And Utility Of An Exome Capture Array For Lentil, Ezgi Ogutcen, Larissa Ramsay, Eric Bishop Von Wettberg, Kirstin E. Bett
College of Agriculture and Life Sciences Faculty Publications
Premise of the Study: Lentil is an important legume crop with reduced genetic diversity caused by domestication bottlenecks. Due to its large and complex genome, tools for reduced representation sequencing are needed. We developed an exome capture array for use in various genetic diversity studies. Methods: Based on the CDC Redberry draft genome, we developed an exome capture array using multiple sources of transcript resources. The probes were designed to target not only the cultivated lentil, but also wild species. We assessed the utility of the developed method by applying the generated data set to population structure and phylogenetic analyses. …
The Impact Of Genetic Changes During Crop Domestication, Petr Smýkal, Matthew N. Nelson, Jens D. Berger, Eric J.B. Von Wettberg
The Impact Of Genetic Changes During Crop Domestication, Petr Smýkal, Matthew N. Nelson, Jens D. Berger, Eric J.B. Von Wettberg
College of Agriculture and Life Sciences Faculty Publications
Humans have domesticated hundreds of plant and animal species as sources of food, fiber, forage, and tools over the past 12,000 years, with manifold effects on both human society and the genetic structure of the domesticated species. The outcomes of crop domestication were shaped by selection driven by human preferences, cultivation practices, and agricultural environments, as well as other population genetic processes flowing from the ensuing reduction in effective population size. It is obvious that any selection imposes a reduction of diversity, favoring preferred genotypes, such as nonshattering seeds or increased palatability. Furthermore, agricultural practices greatly reduced effective population sizes …