Physical Sciences and Mathematics Commons^™

White Blood Cell Dna Methylation And Risk Of Breast Cancer In The Prostate, Lung, Colorectal, And Ovarian Cancer Screening Trial (Plco), Susan R. Sturgeon, J. Richard Pilsner, Kathleen F. Arcaro, Kaoru Ikuma, Haotian Wu, Soon-Mi Kim, Nayha Chopra-Tandon, Adam R. Karpf, Regina G. Ziegler, Catherine Schairer, Raji Balasubramanian, David A. Reckhow

Biostatistics and Epidemiology Faculty Publications Series

Background

Several studies have suggested that global DNA methylation in circulating white blood cells (WBC) is associated with breast cancer risk.

Methods

To address conflicting results and concerns that the findings for WBC DNA methylation in some prior studies may reflect disease effects, we evaluated the relationship between global levels of WBC DNA methylation in white blood cells and breast cancer risk in a case-control study nested within the Prostate, Lung, Colorectal and Ovarian Cancer Screening Trial (PLCO) cohort. A total of 428 invasive breast cancer cases and 419 controls, frequency matched on age at entry (55–59, 60–64, 65–69, ≥70 …

Comparative Study Of Multicellular Tumor Spheroid Formation Methods And Implications For Drug Screening, Maria F. Gencoglu, Lauren E. Barney, Christopher L. Hall, Elizabeth A. Brooks, Alyssa D. Schwartz, Daniel C. Corbett, Kelly R. Stevens, Shelly Peyton

Chemical Engineering Faculty Publication Series

Improved in vitro models are needed to better understand cancer progression and bridge the gap between in vitro proof-of-concept studies, in vivo validation, and clinical application. Multicellular tumor spheroids (MCTS) are a popular method for three-dimensional (3D) cell culture, because they capture some aspects of the dimensionality, cell–cell contact, and cell–matrix interactions seen in vivo. Many approaches exist to create MCTS from cell lines, and they have been used to study tumor cell invasion, growth, and how cells respond to drugs in physiologically relevant 3D microenvironments. However, there are several discrepancies in the observations made of cell behaviors when comparing …