Physical Sciences and Mathematics Commons^™

Protein-Nanoparticle Co-Engineering: Self-Assembly, Intracellular Protein Delivery, And Crispr/Cas9-Based Gene Editing, Rubul Mout

Doctoral Dissertations

Direct cytoplasmic delivery of gene editing nucleases such CRISPR/Cas9 systems and therapeutic proteins provides enormous opportunities in curing human genetic diseases, and assist research in basic cell biology. One approach to attain such a goal is through engineering nanotechnological tools to mimic naturally existing intra- and extracellular protein delivery/transport systems. Nature builds transport systems for proteins and other biomolecules through evolution-derived sophisticated molecular engineering. Inspired by such natural assemblies, I employed molecular engineering approaches to fabricate self-assembled nanostructures to use as intracellular protein delivery tools. Briefly, proteins and gold nanoparticles were co-engineered to carry complementary electrostatic recognition elements. When these …

Influence Of Biomimetic Chelating Packaging On Natural Antimicrobial Efficacy, Paul Castrale

Masters Theses

The iron chelating molecule, ethylenediaminetetraacetic acid (EDTA) is used in food applications for the preservation of oxidation prone ingredients. Research has suggested that EDTA is also capable of enhancing the antimicrobial effectiveness of various compounds including naturally-derived antimicrobials. With consumer demand for cleaner food labels, there remains an opportunity to introduce new chelating technology to replace synthetically-derived EDTA. Through photographting and chemical conversion, hydroxamic acid ligands were covalently bound to polypropylene films resulting in polypropylene-graft-poly(hydroxamic acid) (PP-g-PHA). The resulting films demonstrated an ability to chelate 64 nmol/cm² from an iron saturated environment or 163 nmol/cm …

Modelling Bird Migration With Motus Data And Bayesian State-Space Models, Justin Baldwin

Masters Theses

Bird migration is a poorly-known yet important phenomenon, as understanding movement patterns of birds can inform conservation strategies and public health policy for animal-borne diseases. Recent advances in wildlife tracking technology, in particular the Motus system, have allowed researchers to track even small flying birds and insects with radio transmitters that weigh fractions of a gram. This system relies on a community-based distributed sensor network that detects tagged animals as they move through the detection nodes on journeys that range from small local movements to intercontinental migrations. The quantity of data generated by the Motus system is unprecedented, is on …

Multiple Testing Correction With Repeated Correlated Outcomes: Applications To Epigenetics, Katie Leap

Masters Theses

Epigenetic changes (specifically DNA methylation) have been associated with adverse health outcomes; however, unlike genetic markers that are fixed over the lifetime of an individual, methylation can change. Given that there are a large number of methylation sites, measuring them repeatedly introduces multiple testing problems beyond those that exist in a static genetic context. Using simulations of epigenetic data, we considered different methods of controlling the false discovery rate. We considered several underlying associations between an exposure and methylation over time.

We found that testing each site with a linear mixed effects model and then controlling the false discovery rate …

Tracking 19th Century Late Blight From Archival Documents Using Text Analytics And Geoparsing, Laura Tateosian, Rachael Guenter, Yi-Peng Yang, Jean Ristaino

Free and Open Source Software for Geospatial (FOSS4G) Conference Proceedings

In 1845, Ireland's potato crop was struck by a devastating potato disease that killed Ireland’s crop caused devastation for seven years and led to mass starvation and emigration from the country. The cause of the potato destruction was a fungus-like plant pathogen. There are several theories about the origin of the disease and the source of the 19th century outbreaks. We use historical documents contemporary to that time to investigate spatial information that might inform these mysteries. We present methodologies for automatically extracting information from these voluminous data sources. We identify and map geographic locations that are proximate in the …

Processing Conservation Indicators With Open Source Tools: Lessons Learned From The Digital Observatory For Protected Areas, Lucy Bastin, Andrea Mandrici, Luca Battistella, Grégoire Dubois

Free and Open Source Software for Geospatial (FOSS4G) Conference Proceedings

The European Commission has a commitment to open data and the support of open source software and standards. We present lessons learnt while populating and supporting the web and map services that underly the Joint Research Centre's Digital Observatory for Protected Areas. Challenges include: large datasets with highly complex geometries; topological inconsistencies, compounded by reprojection for equal-area calculations; multiple different representations of the same geographical entities, for example coastlines; licensing requirement to continuously update indicators to respond to monthly changes in the authoritative data. In order to compute and publish an array of indicators, we used a range of open …

Botswana’S Elephant-Back Safari Industry – Stress-Response In Working African Elephants And Analysis Of Their Post-Release Movements, Tanya Lama

Masters Theses

Understanding how African elephants (Loxodonta africana) respond to human interactions in ecotourism operations is critical to safeguarding animal and human welfare and sustaining wildlife ecotourism activities. We investigated the stress response of elephants to a variety of tourist activities over a 15-month period at Abu Camp in northern Botswana. We compared fecal glucocorticoid metabolite (FGM) concentrations across three elephant groups, including: eight elephants in an elephant-tourism operation (Abu herd), three elephants previously reintroduced back into the wild from the Abu herd, and wild elephants. There were no differences in FGM concentrations between the three groups of elephants. The highest observed …

Mechanistic Studies Of Peptide-Mediated Protein Transport Across Droplet-Interface Bilayers, Jing Huang

Doctoral Dissertations

Pep-1 is a promising peptide tool that delivers proteins and peptides into cells with conserved bioactivity. Pep-1 has great potential because of the high efficiency and lack of toxicity. The mechanism of Pep-1-mediated transport is not fully understood. In my thesis, droplet-interface bilayer (DIB) has been used for the mechanistic studies of Pep-1. Here, DIB is developed for different functions such as quantitation of protein translocation, solution exchange to a formed bilayer and simultaneous observation of multiple membranes. Research work on Pep-1 with DIB reveals that the negative charge of the inner membrane leaflet plays a significant role in promoting …

Statistical Methods For High Dimensional Data Arising From Large Epidemiological Studies, Hui Xu

Doctoral Dissertations

In this thesis, we propose statistical models for addressing commonly encountered data types and study designs in large epidemiologic investigations aimed at understanding the molecular basis of complex disorders. The motivating applications come from diverse disease areas in Women's Health, including the study of type II diabetes in the Women's Health Initiative (WHI), invasive breast cancer in the Nurses' Health Study and the study of the metabolomic underpinnings of cardiovascular disease in the WHI. We have also put significant effort into making the implementation of the proposed methods accessible through freely available, user-friendly software packages in R. The first chapter …

Relationship Between Structure And Function In Nickel Proteins And Enzymes, Carolyn Carr

Doctoral Dissertations

Nickel is a rarely used but biologically important metal that is utilized in all three domains of life. In nickel utilizing organisms there is a corresponding trafficking system specifically designed to capture nickel, deliver, and export excess nickel to prevent toxic effects. It is critical to understand the mechanisms by which organisms achieve metal selectivity to duplicate or disrupt this process for the benefit of human health and to further understanding of regulation mechanisms in biology. RcnR is a Ni(II) and Co(II) responsive transcriptional regulator in E. coli. The research reported in this dissertation focuses on the relationship between …

Vitreous Gel Physics, Svetlana Morozova

Doctoral Dissertations

The transparent vitreous, which fills the posterior cavity of the eye, is incredibly engineered. The charged polyelectrolyte hyaluronic acid (HA) network swells to maintain the pressure in the eye, while stiff collagen type II bundles help absorb any external mechanical shock. Our investigations have contributed to a few key developments related to the physical properties of the vitreous: (1) The stiff collagen network that supports the soft gel network is self-assembled from single triple-helix collagen proteins. Electrostatic interactions drive this assembly, such that the size and concentration are optimized at physiological salt concentrations. The width of the assemblies remarkably changes …

Mechanistic Studies Of Proton Gradient-Driven Protein Translocation By Droplet-Interface Bilayer Techniques, En-Hsin Lee

Doctoral Dissertations

Transmembrane proton gradient plays a fundamental role in protein translocation across cellular membranes, including the transport of secreted enzymes from bacterial pathogens into host cells. Much attention has been devoted to understanding the machinery of such delivery and how it functions. Over the past decade, translocation of anthrax toxin has been widely studied not only because of its central role in the deadly pathogenesis of Bacillus anthracis, but also because that it is one of the most tractable toxins and thus serves as an attractive model for studying the translocation machinery that is dependent on proton gradient across membrane. …

Thiol-Ene Chemistry As An Enabler Of New Polymer Structures And Architectures, Joel M. Sarapas

Doctoral Dissertations

This dissertation focuses on two distinct projects: the synthesis and design of novel cell penetrating peptides mimics (CPPMs), and the implementation of the thiol-ene click reaction to generate new polymer architectures and chemistries. Guanidinium-rich CPPMs were generated through both ROMP and RAFT polymerizations, allowing for a comparison to be made across polymer backbone chemistries with respect to both siRNA and protein cellular internalization. A particularly effective methacrylate derived block copolymer was able to deliver siRNA to nearly an entire Jurkat T cell population. The thiol-ene reaction was implemented initially within the context of improving material design for solid polymer electrolytes …

Investigating Patterns Using Analyzing Digital Images (Adi) Software, Jennifer Welborn, Wayne Kermenski

Patterns Around Us

The mean and variation of a population are easily seen by graphing the number of individuals that have a trait of a given value. In this experiment, we will investigate color variation found in a mealworm population using ADI.

In this investigation, we will use digital images and ADI to measure the increased surface area created by villi located in the small intestines.

Wrinkling Of A Floating Sheet, Narayanan Menon

Patterns Around Us

The objective of today’s work will be to generate wrinkle patterns on very thin polymer films using the forces generated by the surface tension of a water drop. You’ll do a few sizes of water drop and we’ll give you a couple of thicknesses of film. Digital images of the pattern will allow us to make observations of the number and size of the wrinkles generated. We will try to develop an understanding of the dependence of the pattern on the materials used and the forces applied

Branching In Nature, Jennifer Welborn, Wayne Kermenski

Patterns Around Us

No abstract provided.

Wrinkling In Nature, Jennifer Welborn, Wayne Kermenski

Patterns Around Us

This lab experience is separated into four investigative segments:

1. Human saliva and enzymes

2. Osmosis and Dialysis Tubing

3. Analyzing dry and imbibed weights of wrinkled and smooth pea seeds

4. Putting it All Together, What makes Wrinkled Peas?

Overview, Benjamin Davidovitch, Narayanan Menon, Jennifer Welborn, Wayne Kermenski

Patterns Around Us

No abstract provided.

Superhero Robotics, Frank Sup, Brian Umberger, Nick Sawyer

Science and Engineering Saturday Seminars

No abstract provided.

From Planning To Passing: The Amherst, Massachusetts Plastic Bag Ban, Kevin J. Hollerbach

Student Showcase

This case study will detail the background and influences for a plastic bag ban in the town of Amherst, outline the steps taken from planning to passage, and address roadblocks and missteps that may be avoided with the implementation of future bans. It is my hope that this document will not only serve as a guide, but also an inspiration for additional local action in Massachusetts and across the country. No matter what the national political climate or attitude towards environmental issues, local action is always possible, and change is usually easier than you think. Think globally, act locally!