Open Access. Powered by Scholars. Published by Universities.®
Physical Sciences and Mathematics Commons™
Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- 2-oxoglutarate (1)
- 2OG (1)
- 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (1)
- Apoptosis (1)
- Arsenic (1)
-
- Beta-lactam (1)
- C-terminal transactivation domain (1)
- CODD (1)
- Chiral (1)
- Clustering (1)
- Deamidation (1)
- Dipole (1)
- Emission (1)
- Field Analysis (1)
- Fluorescence (1)
- Fluorescent (1)
- GFP (1)
- GST (1)
- Glutathione S-transferase (1)
- HEPES (1)
- Hydrogen bonding (1)
- Imidazole (1)
- Ion molecule reactions (1)
- Ion trap (1)
- Mass Spectrometry (1)
- Mass spectrometry (1)
- Metal binding (1)
- Miniaturization (1)
- Multiplexed MS/MS (1)
- N-terminal transactivation domain (1)
Articles 1 - 9 of 9
Full-Text Articles in Physical Sciences and Mathematics
Clustering, Reorientation Dynamics, And Proton Transfer In Glassy Oligomeric Solids, Jacob Allen Harvey
Clustering, Reorientation Dynamics, And Proton Transfer In Glassy Oligomeric Solids, Jacob Allen Harvey
Open Access Dissertations
We have modelled structures and dynamics of hydrogen bond networks that form from imidazoles tethered to oligomeric aliphatic backbones in crystalline and glassy phases. We have studied the behavior of oligomers containing 5 or 10 imidazole groups. These systems have been simulated over the range 100-900 K with constantpressure molecular dynamics using the AMBER 94 force field, which was found to show good agreement with ab initio calculations on hydrogen bond strengths and imidazole rotational barriers. Hypothetical crystalline solids formed from packed 5-mers and 10-mers melt above 600 K, then form glassy solids upon cooling. Viewing hydrogen bond networks as …
Reactive Heterocycles For Examining Polyketide Biosynthesis, Gitanjeli Prasad
Reactive Heterocycles For Examining Polyketide Biosynthesis, Gitanjeli Prasad
Open Access Dissertations
Polyketides are a class of natural products that exhibit remarkable structural and functionally diversity and are highly sought after due to their medicinally important activities. For many decades now, polyketide synthases (PKSs), the mega-enzymes responsible for biosynthesis of polyketides have been the focus of extensive investigation to make new polyketides by polyketide engineering strategies. While there are many established methods to investigate polyketide enzymes and biosynthesis mechanisms, they have substantial shortcomings that have limited the extent of success with polyketide engineering efforts.
This thesis focuses on developing simple, flexible yet powerful tools for examining polyketide biosynthesis by overcoming some deficiencies …
O2 Activation And Allosteric Zn(Ii) Binding On Hif-Prolyl Hydroxylase-2 (Phd2), Serap Pektas
O2 Activation And Allosteric Zn(Ii) Binding On Hif-Prolyl Hydroxylase-2 (Phd2), Serap Pektas
Open Access Dissertations
Oxygen homeostasis is essential to the life of aerobes, which is regulated in humans by Hypoxia Inducible Factor-1α (HIF-1α). Under hypoxic conditions, HIF-1α transactivates over a hundred genes related angiogenesis, erythropoiesis, etc. HIF-1α level and function is regulated by four HIF hydroxylase enzymes: three isoforms of prolyl hydroxylase domain (PHD1, PHD2 and PHD3) and factor inhibiting HIF-1α (FIH). PHD2 is the focus of this research. PHD2 is a non-heme Fe(II) 2-oxoglutarate dependent dioxygenase, which controls HIF-1α levels by hydroxylating two proline residues within the ODD domain of HIF-1α, then the hydroxylated prolines are recognized by pVHL, which targets HIF-1α for …
Development Of Mass Spectrometry-Based Methods For Quantitation And Characterization Of Protein Drugs: Transferrin As A Model Drug Delivery Vehicle, Shunhai Wang
Open Access Dissertations
In the last two decades, protein drugs have enjoyed a rapid growth and achieved a tremendous success in treating human diseases. However, the presence of physiological barriers greatly impedes the efficient delivery of such unconventional large molecule drugs, and therefore limits their clinical utility. An elegant way to address this challenge takes advantage of certain endogenous transporter proteins, such as human transferrin (Tf), whose ability to traverse physiological barriers has been extensively exploited. However, methods to investigate Tf-based drug delivery remained insufficient and unsatisfactory until recent development of quantitative mass spectrometry (MS). Hereby, MS-based methods have been developed and validated …
Enhanced Detection Strategies Accomplished Through Metal Binding And Miniature Mass Spectrometry, Adam Graichen
Enhanced Detection Strategies Accomplished Through Metal Binding And Miniature Mass Spectrometry, Adam Graichen
Open Access Dissertations
A multiplexed method for performing MS/MS on multiple ions simultaneously in a miniature rectilinear ion trap (RIT) mass spectrometer has been developed. This method uses an ion encoding procedure that relies on the mass bias that exists when ions are externally injected into an RIT operated with only a single phase RF applied to one pair of electrodes. The ion injection profile under such conditions ions is Gaussian-like over a wide range of RF amplitudes, or low mass cutoff (LMCO) values, during ion accumulation. We show that this distribution is related to ion m/z and is likely caused by ions …
Investigation Of The Orientation Dependence On Chiroptical Properties Of Single Molecules, Austin Joseph Cyphersmith
Investigation Of The Orientation Dependence On Chiroptical Properties Of Single Molecules, Austin Joseph Cyphersmith
Open Access Dissertations
Optical activity is the defining property of chiral materials that is essential for characterization in biology, chemistry, and physics. While a substantial body of research has provided a strong theoretical framework of the origin of optical activity, we still know very little by way of experiment about an individual molecule’s contribution to the bulk optical activity. The chiroptical response of a single molecule can depend on molecular orientation and local molecular environment, information which is lost in ensemble averaging.
This thesis focuses on establishing methods for a priori determination of chiral molecule orientations and refining measurements to probe the chiroptical …
Determination Of Arsenic In Water By Potentially Portable Methodology, Chengbei Li
Determination Of Arsenic In Water By Potentially Portable Methodology, Chengbei Li
Open Access Dissertations
Arsenic contamination in groundwater is a worldwide problem. The existing portable field test kits can not provide accurate results when the arsenic concentration is around 10 μg L-1 or lower. This research first was focused on the development and validation of methods in which portable instrumentation, such as electrochemistry instruments or quartz crystal microbalances, could be used to accurately determine arsenic concentrations in water even when the concentration is below 10 μg L-1. A modified anodic stripping voltammetry (ASV) and cathodic stripping voltammetry (CSV) method with measurement at a microarray electrode manufactured by TraceDetect Inc. was developed. When the ASV …
Assembly Of Surface Engineered Nanoparticles For Functional Materials, Xi Yu
Assembly Of Surface Engineered Nanoparticles For Functional Materials, Xi Yu
Open Access Dissertations
Nanoparticles are regarded as exciting new building blocks for functional materials due to their fascinating physical properties because of the nano-confinement. Organizing nanoparticles into ordered hierarchical structures are highly desired for constructing novel optical and electrical artificial materials that are different from their isolated state or thermodynamics random ensembles. My research integrates the surface chemistry of nanoparticles, interfacial assembly and lithography techniques to construct nanoparticle based functional structures. We designed and synthesized tailor-made ligands for gold, semiconductor and magnetic nanoparticle, to modulate the assembly process and collective properties of the assembled structures, by controlling the key parameters such as particle-interface …
Development And Characterization Of Caspase Activatable Gfp And A Family Of Fluorescent Reporters, Samantha Elizabeth Bernard Nicholls
Development And Characterization Of Caspase Activatable Gfp And A Family Of Fluorescent Reporters, Samantha Elizabeth Bernard Nicholls
Open Access Dissertations
The cellular process of programmed cell death, or apoptosis, is critical in homeostasis and development. In addition it's misfunction is implicated in an array of disease states from cancer to neurodegeration, making it an attractive pathway for drug targeting. A family of proteases, known as caspases, plays a central role in the apoptotic cascade resulting in the ultimate destruction of the cell. We report a genetically encoded dark-to-bright reporter of caspase activity used in E.coli, mammalian cells, and whole organisms which can be used to monitor apoptosis. This reporter, caspase activatable green fluorescent protein (CA-GFP) consists of GFP fused through …