Physical Sciences and Mathematics Commons^™

Effects Of Precipitation Increase On Soil Respiration: A Three-Year Field Experiment In Subtropical Forests In China, Qi Deng, Dafeng Hui, Deqiang Zhang, Guoyi Zhou, Juxiu Liu, Shizhong Liu, Guowei Chu, Jiong Li

Biology Faculty Research

Background

The aim of this study was to determine response patterns and mechanisms of soil respiration to precipitation increases in subtropical regions.

Methodology/Principal Findings

Field plots in three typical forests [i.e. pine forest (PF), broadleaf forest (BF), and pine and broadleaf mixed forest (MF)] in subtropical China were exposed under either Double Precipitation (DP) treatment or Ambient Precipitation (AP). Soil respiration, soil temperature, soil moisture, soil microbial biomass and fine root biomass were measured over three years. We tested whether precipitation treatments influenced the relationship of soil respiration rate (R) with soil temperature (T) and soil …

Lipid Peroxides And Glutathione Status In Human Progenitor Mononuclear (U937) Cells Following Exposure To Low Doses Of Nickel And Copper, William Y. Boadi, Shalandus Harris, Justin B. Anderson, Samuel E. Adunyah

Chemistry Faculty Research

Effects of Cu2+, Ni2+ or Cu2+ + Ni2+ on lipid peroxide and glutathione (GSH) levels in U937 cells were investigated. Cells were treated with 0, 5, 10, and 20 µM of Cu2+ and/or Ni2+ and H2O2 (0.01 mM) and incubated for 24 hours at 37°C. Lipid peroxides were measured by the thiobarbituric acid assay (TBA). GSH intracellular levels were assayed by the GSH assay kit from EMD/Calbiochem (San Diego, California, USA). Cu2+ or Ni2+ significantly (P < 0.01) increased lipid peroxides in a dose-dependent manner, compared to controls. The effect was more pronounced for Cu2+, compared to the Ni2+-treated samples. Cu2+ + Ni2+ increased lipid peroxides in a significant (P < 0.001), dose-dependent manner, compared to Cu2+ or Ni2+ alone (i.e., ratio of 2.5:1-fold for combined versus single treatments, respectively). Cu2+ or Ni2+ significantly decreased GSH levels in U937 cells, with the effect being pronounced for Cu2+. Cu2+ + Ni2+ metal ions significantly (P < 0.001) depleted cells of GSH in a dose-dependent manner. Ethylene diamine tetraacetic acid (EDTA) at 50 or 100 µM moderately reduced the Cu2+- or Ni2+-induced effects on GSH levels. Interestingly, GSH levels generally decreased to half (except for the combined metal dose of 20 µM at 100 µM EDTA) of its level at the highest metal concentration tested for both the single or combined treatments. In conclusion, multiple exposures of cells to metal ions may be lethal to cells, compared to their single treatments.

Copyright Page

The Journal of Tennessee State University

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Table Of Contents

The Journal of Tennessee State University

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Forward

The Journal of Tennessee State University

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The Economic Impact Of Tennessee State University On The State Of Tennessee 2010-2011, Soumendra Ghosh, Anis Mnif, Sabeen Fahim

The Journal of Tennessee State University

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Editorial Board Page

The Journal of Tennessee State University

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Some Notable Writers, Editors, And Publishers Affiliated With Tsu Over Its First 100 Years

The Journal of Tennessee State University

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Living Our Lives As Elders: A Convocation Address, Celeste C. Williams

The Journal of Tennessee State University

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Dr. Thomas E. Poag: The Life And Works Of A Tsu Legend, Helen R. Houston

The Journal of Tennessee State University

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Service-Learning And Civic Engagement: A Return To Our Roots, Deena Sue Fuller

The Journal of Tennessee State University

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Coach Ed Temple And The Tigerbelles, Yildiz B. Binkley, Reavis Mitchell, Tara Mitchell Mielnik

The Journal of Tennessee State University

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Past To Present: School Of Allied Health Professions To College Of Health Sciences And Contributions To Health Improvement In The Local Community And Beyond, Mohamed Kanu, Ronald Barredo, Elizabeth Williams, Revlon Briggs

The Journal of Tennessee State University

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A Royal Band, The Chosen Few: The Birth Of Tennessee State University, Crystal A. Degregory

The Journal of Tennessee State University

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Tennessee State University: A Synopsis Of “A Touch Of Greatness,” 1912-2012, Bobby L. Lovett

The Journal of Tennessee State University

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