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Full-Text Articles in Physical Sciences and Mathematics
An Integrated Systems Approach To Deconstructing Glycosylation, L. K. Mahal, John F. Rakus, Kanoelani Pilobello, P. Agrawal
An Integrated Systems Approach To Deconstructing Glycosylation, L. K. Mahal, John F. Rakus, Kanoelani Pilobello, P. Agrawal
Chemistry Faculty Research
Glycosylation involves the post-translational addition of carbohydrates to protein molecules and is an intricate and indispensable biochemical process. Study of this complicated network of interactions is hindered by the lack of a coding template analogous to the genetic code, and by the vast structural complexity inherent to carbohydrate polymers. We use lectins (non-enzymatic carbohydrate-binding proteins of non-immunological origin) as microarray probes to identify carbohydrate features expressed on cellular surfaces. Specifically, we utilized lectin microarray technology to investigate the differences in carbohydrates expressed by the cell lines of the Nation Cancer Institute’s NCI-60 panel. Our investigation identified tissue-specific expression differences in …
A Systems Approach To Understanding The Role Of Glycans In Cancer, John F. Rakus, Kaneolani T. Pilobello, Laura K. Mahal
A Systems Approach To Understanding The Role Of Glycans In Cancer, John F. Rakus, Kaneolani T. Pilobello, Laura K. Mahal
Chemistry Faculty Research
Glycosylation involves the post-translational addition of carbohydrates to protein molecules and is an intricate and indispensable biochemical process. Study of this complicated network of interactions is hindered by the lack of a coding template analogous to the genetic code, and by the vast structural complexity inherent to carbohydrate polymers. We use lectins (non-enzymatic carbohydrate-binding proteins of non-immunological origin) as microarray probes to identify carbohydrate features expressed on cellular surfaces. Specifically, we utilized lectin microarray technology to investigate the differences in carbohydrates expressed by the cell lines of the Nation Cancer Institute’s NCI-60 panel. Our investigation identified tissue-specific expression differences in …