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- Keyword
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- Buffer; catalase; gluconic acid; glucose; hydrogen peroxide; oxygen; glucose oxidase; reducing agent; article; catalysis; cell respiration; chemical reaction; decomposition; enzyme mechanism; glucose oxidation; kinetics; mitochondrial respiration; nonhuman; oxidation kinetics; oxidation reduction reaction; oxygen concentration; oxygen saturation; phosphorescence; proton nuclear magnetic resonance; respiratory chain; respiratory chain (1)
- Catalytic effects; Distilled water; Dithionite; Fast oxidation; Large surface area; Mesoporosity; Mesoporous SBA-15; Mesoporous Silica; Mesoporous silica nanoparticles; Nano-materials; Neutral solution; Oxidative process; Oxygen radical; PBS solution; PH-dependent; Phosphate buffers; Phosphate-buffered salines; Postsynthesis; Silica nanoparticles; Silica Sphere; Solid silica; Surface area; Templating (1)
- Chemistry (1)
- Mcm 41; microsphere; nanomaterial; SBA 15; silica microsphere; silicon dioxide; unclassified drug; article; cell damage; cell death; cell viability; controlled study; cytotoxicity; endocytosis; human; human cell; incubation time; mesoporosity; physical parameters; porosity; transmission electron microscopy (1)
- Oxidative phosphorylation and cellular energetics; Aspergillus niger; biocatalysis; enzymology; metabolism (1)
Articles 1 - 5 of 5
Full-Text Articles in Physical Sciences and Mathematics
Mesoporosity And Functional Group Dependent Endocytosis And Cytotoxicity Of Silica Nanomaterials, Zhimin Tao, Bonnie B. Toms, Jerry Goodisman, Tewodros Asefa
Mesoporosity And Functional Group Dependent Endocytosis And Cytotoxicity Of Silica Nanomaterials, Zhimin Tao, Bonnie B. Toms, Jerry Goodisman, Tewodros Asefa
Chemistry - All Scholarship
We report different mesoporosity-dependent and functional group-dependent cytotoxicity and endocytosis of various silica nanomaterials on suspended and adherent cells. This dependency further varied with incubation time and particle dosage, and appeared to be associated with the particles' endocytotic efficiency and their chemical and physical properties. We studied two common mesoporous nanomaterials (MSNs), MCM-41 and SBA-15, and one type of solid-cored silica microsphere, paralleled by their quaternary amine functionalized counterparts. Compared to SBA-15, MCM-41 has a larger surface area but smaller pore size, whereas SMS exhibits low surface area and poor porosity. In Jurkat cells, SBA-15 and MCM-41 exhibited different cytotoxicity …
Mesoporosity And Functional Group Dependent Endocytosis And Cytotoxicity Of Silica Nanomaterials, Zhimin Tao, Jerry Goodisman, Tewodros Asefa, Bonnie B. Toms
Mesoporosity And Functional Group Dependent Endocytosis And Cytotoxicity Of Silica Nanomaterials, Zhimin Tao, Jerry Goodisman, Tewodros Asefa, Bonnie B. Toms
Chemistry - All Scholarship
We report different mesoporosity-dependent and functional group-dependent cytotoxicity and endocytosis of various silica nanomaterials on suspended and adherent cells. This dependency further varied with incubation time and particle dosage, and appeared to be associated with the particles’ endocytotic efficiency and their chemical and physical properties. We studied two common mesoporous nanomaterials (MSNs), MCM-41 and SBA-15, and one type of solid-cored silica microsphere, paralleled by their quaternary amine functionalized counterparts. Compared to SBA-15, MCM-41 has a larger surface area but smaller pore size, whereas SMS exhibits low surface area and poor porosity. In Jurkat cells, SBA-15 and MCM- 41 exhibited different …
Simultaneous, Noninvasive Observation Of Elastic Scattering, Fluorescence And Inelastic Scattering As A Monitor Of Blood Flow And Hematocrit In Human Fingertip Capillary Beds, Joseph Chaiken, Jerry Goodisman, Bin Deng, Rebecca J. Bussjager, George Shaheen
Simultaneous, Noninvasive Observation Of Elastic Scattering, Fluorescence And Inelastic Scattering As A Monitor Of Blood Flow And Hematocrit In Human Fingertip Capillary Beds, Joseph Chaiken, Jerry Goodisman, Bin Deng, Rebecca J. Bussjager, George Shaheen
Chemistry - All Scholarship
We report simultaneous observation of elastic scattering, fluorescence, and inelastic scattering from in vivo near-infrared probing of human skin. Careful control of the mechanical force needed to obtain reliable registration of in vivo tissue with an appropriate optical system allows reproducible observation of blood flow in capillary beds of human volar side fingertips. The time dependence of the elastically scattered light is highly correlated with that of the combined fluorescence and Raman scattered light. We interpret this in terms of turbidity (the impeding effect of red blood cells on optical propagation to and from the scattering centers) and the changes …
Accelerated Oxidation Of Epinephrine By Silica Nanoparticles, Zhimin Tao, Gang Wang, Jerry Goodisman, Tewodros Asefa
Accelerated Oxidation Of Epinephrine By Silica Nanoparticles, Zhimin Tao, Gang Wang, Jerry Goodisman, Tewodros Asefa
Chemistry - All Scholarship
We have measured the influence of mesoporous silica (MCM-41 and SBA-15) nanoparticles and dense silica nanoparticles on epinephrine oxidation, a pH-dependent reaction, whose rate is small in acidic or neutral solutions but much greater at higher pH. The reaction was measured by monitoring adrenochrome at 480 nm, the product of epinephrine oxidation. In distilled water (dH 2O) with no particles present, the oxidation of epinephrine occurs slowly but more rapidly at higher pH. The presence of MCM-41 or silica spheres does not accelerate the oxidation, but SBA-15 does, showing that the difference in the structures of nanomaterials leads to …
Kinetic Studies On Enzyme-Catalyzed Reactions: Oxidation Of Glucose, Decomposition Of Hydrogen Peroxide And Their Combination, Zhimin Tao, Ryan A. Raffel, Abdul-Kader Souid, Jerry Goodisman
Kinetic Studies On Enzyme-Catalyzed Reactions: Oxidation Of Glucose, Decomposition Of Hydrogen Peroxide And Their Combination, Zhimin Tao, Ryan A. Raffel, Abdul-Kader Souid, Jerry Goodisman
Chemistry - All Scholarship
The kinetics of the glucose oxidase-catalyzed reaction of glucose with O2, which produces gluconic acid and hydrogen peroxide, and the catalase-assisted breakdown of hydrogen peroxide to generate oxygen, have been measured via the rate of O2 depletion or production. The O2 concentrations in air-saturated phosphate-buffered salt solutions were monitored by measuring the decay of phosphorescence from a Pd phosphor in solution; the decay rate was obtained by fitting the tail of the phosphorescence intensity profile to an exponential. For glucose oxidation in the presence of glucose oxidase, the rate constant determined for the rate-limiting step was k = (3.0 ± …