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Full-Text Articles in Physical Sciences and Mathematics
Synthesis Of Adenosine Derivatives As Transcription Initiators And Preparation Of 5 ' Fluorescein- And Biotin-Labeled Rna Through One-Step In Vitro Transcription, Faqing Huang, Guocan Wang, Tricia Coleman, Na Li
Synthesis Of Adenosine Derivatives As Transcription Initiators And Preparation Of 5 ' Fluorescein- And Biotin-Labeled Rna Through One-Step In Vitro Transcription, Faqing Huang, Guocan Wang, Tricia Coleman, Na Li
Faculty Publications
Expanding our previous finding of an adenosine-initiated transcription system, we now demonstrate that either the 5' site or the N6 site of adenosine nucleotides can be modified extensively without abolishing their ability to initiate transcription under the T7 phi2.5 promoter. Two series of amino derivatives of adenosine nucleotides were synthesized. Fluorescein and biotin groups were coupled to AMP derivatives through linkers of different sizes and hydrophobicities. Both fluorescein- and biotin-conjugated (at either the 5' or N6 site) adenosine nucleotides can act as efficient transcription initiators, producing fluorescein- and biotin-labeled RNA at the specific 5' end by a one-step transcription procedure, …
Markov Chains And Dynamic Geometry Of Polygons, Jiu Ding, L. Richard Hitt, Xin-Min Zhang
Markov Chains And Dynamic Geometry Of Polygons, Jiu Ding, L. Richard Hitt, Xin-Min Zhang
Faculty Publications
In this paper we construct sequences of polygons from a given n-sided cyclic polygon by iterated procedures and study the limiting behaviors of these sequences in terms of non-negative matrices and Markov chains. (C) 2003 Elsevier Science Inc. All rights reserved.
Efficient Incorporation Of Coa, Nad And Fad Into Rna By In Vitro Transcription, Faqing Huang
Efficient Incorporation Of Coa, Nad And Fad Into Rna By In Vitro Transcription, Faqing Huang
Faculty Publications
Protein enzymes frequently recruit small molecule coenzymes to perform a variety of biochemical reactions. While the catalytic activities of RNA have been expanding rapidly, a similar strategy for RNA to utilize coenzymes and to increase its functional capabilities has yet to be demonstrated. A general in vitro transcription procedure has been developed to efficiently prepare RNA with coenzymes CoA, NAD and FAD covalently attached to the 5' end. These adenosine-containing coenzymes initiate transcription under the T7 class II promoter by T7 RNA polymerase. In addition to the three coenzymes, other adenosine-containing molecules may be incorporated into the first nucleotide position …
Crystal Structure Of Boc-Trp(Cho)-Dphe-Ile-Dphe-Leu-Och3, C48h59n6o10, Vijayaraghavan Rangachari, J. Makker, P. Kumar, S. Dey, Tej P. Singh
Crystal Structure Of Boc-Trp(Cho)-Dphe-Ile-Dphe-Leu-Och3, C48h59n6o10, Vijayaraghavan Rangachari, J. Makker, P. Kumar, S. Dey, Tej P. Singh
Faculty Publications
No abstract provided.