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Demonstration Of Tritrichomonas Foetus In The External Genitalia And Of Specific Antibodies In Preputial Secretions Of Naturally Infected Bulls, J. C. Rhyan, K. L. Wilson, B. Wagner, M. L. Anderson, R. H. Bondurant, D. E. Burgess, G. K. Mutwiri, L. B. Corbeil

USDA Wildlife Services: Staff Publications

Portions of penis and prepuce were collected from 24 bulls with current or recent Tritrichomonas foetus infection. Epididymides were collected from seven of the bulls, and seminal vesicles and prostate were collected from four. Following immunohistochemical staining with two monoclonal antibodies (34.7C4.4 and TF1.15) prepared against T. foetus surface antigens, trichomonads were identified in sections from 15 of the bulls. Organisms were most often located in penile crypts in the midshaft and caudal regions and less often in preputial crypts. Trichomonads were not observed in sections from other genitalia or in subepithelial tissue. T. foetus antigen, however, was present in …