Physical Sciences and Mathematics Commons^™

In Vitro Cytotoxicity Study Of Glutaraldehyde And No-React Treated Bioprosthetic Heart Valves And The Aortic Wall, Roger Ongosi

Theses

Valvular failure due to calcification and leaflet disruption of artificial bioprostheses is still a major concern in valve replacement surgery. Previous studies have shown that Glutaraldehyde, a chemical used in the treatment of artificial valves promotes calcification. In this investigation glutaraldehyde and No-React® treated tissue samples of pericardium, cusp and the aortic wall were tested for cytocompatibility using live mouse fibroblast cultures. The samples were cut into 3 X 3 mm2 washed in phosphate buffered saline solution, transferred into cell culture flask containing cells that had been cultured for 24 hours and incubated at 37 "C in 5 % CO2. …