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Full-Text Articles in Physical Sciences and Mathematics
Synthesis And Characterization Of A Water-Soluble Metallopeptide That Resembles A Parallel Beta-Pleated Sheet, Michael Y. Ogawa, Alexei B. Gretchikhine, Sunil-Datta Soni, Steven M. Davis
Synthesis And Characterization Of A Water-Soluble Metallopeptide That Resembles A Parallel Beta-Pleated Sheet, Michael Y. Ogawa, Alexei B. Gretchikhine, Sunil-Datta Soni, Steven M. Davis
Chemistry Faculty Publications
The title metallopeptide consists of two divalinyl peptides attached to the carboxylate groups of the ruthenium polypyridyl complex. [Ru(bpy)(2)L](2+): bpy = 2,2'-bipyridine; L = 3,5-dicarboxy-2,2'-bipyridine. The charged ruthenium complex is water soluble and is shown by variable temperature, and 2-D NMR to consist a parallel arrangement of interacting, extended peptide strands that ir reminiscent of the beta-sheet structure. Circular dichroism results support this assignment.
Relative Stabilities Of Dna 3-Way, 4-Way And 5-Way Junctions (Multi-Helix Junction Loops) - Unpaired Nucleotides Can Be Stabilizing Or Destabilizing, Neocles B. Leontis, Julie L. Kardmas, Amy J. Ravin
Relative Stabilities Of Dna 3-Way, 4-Way And 5-Way Junctions (Multi-Helix Junction Loops) - Unpaired Nucleotides Can Be Stabilizing Or Destabilizing, Neocles B. Leontis, Julie L. Kardmas, Amy J. Ravin
Chemistry Faculty Publications
Competition binding and UV melting studies of a DNA model system consisting of three, four or five mutually complementary oligonucleotides demonstrate that unpaired bases at the branch point stabilize three- and five-way junction loops but destabilize four-way junctions. The inclusion of unpaired nucleotides permits the assembly of five-way DNA junction complexes (5WJ) having as few as seven basepairs per arm from five mutually complementary oligonucleotides. Previous work showed that 5WJ, having eight basepairs per arm but lacking unpaired bases, could not be assembled I[Wang,Y.L., Mueller,J.E., Kemper,B. and Seeman,N.C. (1991) Biochemistry, 30, 5667-5674]. Competition binding experiments demonstrate that four-way junctions (4WJ) …
Helical Stacking In Dna 3-Way Junctions Containing 2 Unpaired Pyrimidines - Proton Nmr-Studies, Neocles B. Leontis, Michael T. Hills, Martial Piotto, Igor V. Ouporov, Arun Malhotra, David G. Gorenstein
Helical Stacking In Dna 3-Way Junctions Containing 2 Unpaired Pyrimidines - Proton Nmr-Studies, Neocles B. Leontis, Michael T. Hills, Martial Piotto, Igor V. Ouporov, Arun Malhotra, David G. Gorenstein
Chemistry Faculty Publications
The proton NMR spectra of DNA three-way junction complexes (TWJ) having unpaired pyrimidines, 5'-TT- and 5'-TC- on one strand at the junction site were assigned from 2D NOESY spectra acquired in H2O and D2O solvents and homonuclear 3D NOESY-TOCSY and 3D NOESY-NOESY in D2O solvent. TWJ are the simplest branched structures found in biologically active nucleic acids. Unpaired nucleotides are common features of such structures and have been shown to stabilize junction formation. The NMR data confirm that the component oligonucleotides assemble to form conformationally homogeneous TWJ complexes having three double-helical, B-form arms. Two of the helical arms stack upon …
Refinement Of The Solution Structure Of A Branched Dna Three-Way Junction, Neocles B. Leontis, Igor V. Ouporov
Refinement Of The Solution Structure Of A Branched Dna Three-Way Junction, Neocles B. Leontis, Igor V. Ouporov
Chemistry Faculty Publications
We have refined the structure of the DNA Three-Way Junction complex, TWJ- TC, described in the companion paper by quantitative analysis of two 2D NOESY spectra (mixing times 60 and 200 ms) obtained in D2O solution. NOESY crosspeak intensities extracted from these spectra were used in two kinds of refinement procedure: 1) distance-restrained energy minimization (EM) and molecular dynamics (MD) and 2) full relaxation matrix back calculation refinement. The global geometry of the refined model is very similar to that of a published, preliminary model (Leontis, 1993). Two of the helical arms of the junction are stacked. These are Helix …