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A Non-Invasive Neurotoxicity Assay Using Larval Medaka, Steven P. Bradbury, Dave Featherstone, Charles D. Drewes, Joel R. Coats
A Non-Invasive Neurotoxicity Assay Using Larval Medaka, Steven P. Bradbury, Dave Featherstone, Charles D. Drewes, Joel R. Coats
Steven P. Bradbury
We present a method for non-invasive electrophysiological analysis of rapid escape responses in intact, freely behaving larval medaka (Oryzias latipes) before and after short-term exposure to environmental toxicants. Recordings are obtained as a larval medaka swims in a small chamber of water above pairs of recording electrodes etched onto a printed circuit board. When the chamber is briefly vibrated by a sinusoidal pulse from a speaker attached to the grid, a stereotyped Mauthner cell-initiated escape response is evoked. The following parameters were quantified from recordings: 1) Mauthner axon conduction velocity, 2) delay between Mauthner axon spike and motoneuron spike in …